LOCUS       sequence-173029-       13961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic Sindbis virus plasmid for use in SYNseq PLA screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13961)
  AUTHORS   Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, 
            Correa IR Jr, Bressan D, Zador AM
  TITLE     Using high-throughput barcode sequencing to efficiently map 
            connectomes.
  JOURNAL   Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292.
  PUBMED    28449067
REFERENCE   2  (bases 1 to 13961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2017-07-7"; volume: "45(12):e115. doi"; pages: "
            10.1093/nar/gkx292"
COMMENT     Sequence Label: IDP425
FEATURES             Location/Qualifiers
     source          1..13961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        97..201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(202..270,271..1062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(420..439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1233..1821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1722..1741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1909..1927
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1909..1926
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      9579..9588
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9726..9752
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             9759..10304
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      10766..10775
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      12561..12570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12567..12632
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      13018..13027
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13024..13737
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(13169..13187)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     13437..13456
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ggggaattcc tcgataatta agcggccgct taattaatcg aggggaatta attcttgaag
       61 acgaaagggc caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      121 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      181 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      241 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      301 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      361 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      421 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
      481 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      541 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
      601 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
      661 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
      721 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
      781 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
      841 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      901 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
      961 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1021 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1081 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1141 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1201 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1261 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1321 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     1381 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1441 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1501 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1561 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1621 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1681 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1741 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1801 ggggggcgga gcctatggaa aaacgccagc aacgcgagct cgtatggaca tattgtcgtt
     1861 agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat ttaggtgaca
     1921 ctatagattg acggcgtagt acacactatt gaatcaaaca gccgaccaat tgcactacca
     1981 tcacaatgga gaagccagta gtaaacgtag acgtagaccc ccagagtccg tttgtcgtgc
     2041 aactgcaaaa aagcttcccg caatttgagg tagtagcaca gcaggtcact ccaaatgacc
     2101 atgctaatgc cagagcattt tcgcatctgg ccagtaaact aatcgagctg gaggttccta
     2161 ccacagcgac gatcttggac ataggcagcg caccggctcg tagaatgttt tccgagcacc
     2221 agtatcattg tgtctgcccc atgcgtagtc cagaagaccc ggaccgcatg atgaaatatg
     2281 ccagtaaact ggcggaaaaa gcgtgcaaga ttacaaacaa gaacttgcat gagaagatta
     2341 aggatctccg gaccgtactt gatacgccgg atgctgaaac accatcgctc tgctttcaca
     2401 acgatgttac ctgcaacatg cgtgccgaat attccgtcat gcaggacgtg tatatcaacg
     2461 ctcccggaac tatctatcat caggctatga aaggcgtgcg gaccctgtac tggattggct
     2521 tcgacaccac ccagttcatg ttctcggcta tggcaggttc gtaccctgcg tacaacacca
     2581 actgggccga cgagaaagtc cttgaagcgc gtaacatcgg actttgcagc acaaagctga
     2641 gtgaaggtag gacaggaaaa ttgtcgataa tgaggaagaa ggagttgaag cccgggtcgc
     2701 gggtttattt ctccgtagga tcgacacttt atccagaaca cagagccagc ttgcagagct
     2761 ggcatcttcc atcggtgttc cacttgaatg gaaagcagtc gtacacttgc cgctgtgata
     2821 cagtggtgag ttgcgaaggc tacgtagtga agaaaatcac catcagtccc gggatcacgg
     2881 gagaaaccgt gggatacgcg gttacacaca atagcgaggg cttcttgcta tgcaaagtta
     2941 ctgacacagt aaaaggagaa cgggtatcgt tccctgtgtg cacgtacatc ccggccacca
     3001 tatgcgatca gatgactggt ataatggcca cggatatatc acctgacgat gcacaaaaac
     3061 ttctggttgg gctcaaccag cgaattgtca ttaacggtag gactaacagg aacaccaaca
     3121 ccatgcaaaa ttaccttctg ccgatcatag cacaagggtt cagcaaatgg gctaaggagc
     3181 gcaaggatga tcttgataac gagaaaatgc tgggtactag agaacgcaag cttacgtatg
     3241 gctgcttgtg ggcgtttcgc actaagaaag tacattcgtt ttatcgccca cctggaacgc
     3301 agacctgcgt aaaagtccca gcctctttta gcgcttttcc catgtcgtcc gtatggacga
     3361 cctctttgcc catgtcgctg aggcagaaat tgaaactggc attgcaacca aagaaggagg
     3421 aaaaactgct gcaggtctcg gaggaattag tcatggaggc caaggctgct tttgaggatg
     3481 ctcaggagga agccagagcg gagaagctcc gagaagcact tccaccatta gtggcagaca
     3541 aaggcatcga ggcagccgca gaagttgtct gcgaagtgga ggggctccag gcggacatcg
     3601 gagcagcatt agttgaaacc ccgcgcggtc acgtaaggat aatacctcaa gcaaatgacc
     3661 gtatgatcgg acagtatatc gttgtctcgc caaactctgt gctgaagaat gccaaactcg
     3721 caccagcgca cccgctagca gatcaggtta agatcataac acactccgga agatcaggaa
     3781 ggtacgcggt cgaaccatac gacgctaaag tactgatgcc agcaggaggt gccgtaccat
     3841 ggccagaatt cctagcactg agtgagagcg ccacgttagt gtacaacgaa agagagtttg
     3901 tgaaccgcaa actataccac attgccatgc atggccccgc caagaataca gaagaggagc
     3961 agtacaaggt tacaaaggca gagcttgcag aaacagagta cgtgtttgac gtggacaaga
     4021 agcgttgcgt taagaaggaa gaagcctcag gtctggtcct ctcgggagaa ctgaccaacc
     4081 ctccctatca tgagctagct ctggagggac tgaagacccg acctgcggtc ccgtacaagg
     4141 tcgaaacaat aggagtgata ggcacaccgg ggtcgggcaa gtcagctatt atcaagtcaa
     4201 ctgtcacggc acgagatctt gttaccagcg gaaagaaaga aaattgtcgc gaaattgagg
     4261 ccgacgtgct aagactgagg ggtatgcaga ttacgtcgaa gacagtagat tcggttatgc
     4321 tcaacggatg ccacaaagcc gtagaagtgc tgtacgttga cgaagcgttc gcgtgccacg
     4381 caggagcact acttgccttg attgctatcg tcaggccccg caagaaggta gtactatgcg
     4441 gagaccccat gcaatgcgga ttcttcaaca tgatgcaact aaaggtacat ttcaatcacc
     4501 ctgaaaaaga catatgcacc aagacattct acaagtatat ctcccggcgt tgcacacagc
     4561 cagttacagc tattgtatcg acactgcatt acgatggaaa gatgaaaacc acgaacccgt
     4621 gcaagaagaa cattgaaatc gatattacag gggccacaaa gccgaagcca ggggatatca
     4681 tcctgacatg tttccgcggg tgggttaagc aattgcaaat cgactatccc ggacatgaag
     4741 taatgacagc cgcggcctca caagggctaa ccagaaaagg agtgtatgcc gtccggcaaa
     4801 aagtcaatga aaacccactg tacgcgatca catcagagca tgtgaacgtg ttgctcaccc
     4861 gcactgagga caggctagtg tggaaaacct tgcagggcga cccatggatt aagcagctca
     4921 ctaacatacc taaaggaaac tttcaggcta ctatagagga ctgggaagct gaacacaagg
     4981 gaataattgc tgcaataaac agccccactc cccgtgccaa tccgttcagc tgcaagacca
     5041 acgtttgctg ggcgaaagca ttggaaccga tactagccac ggccggtatc gtacttaccg
     5101 gttgccagtg gagcgaactg ttcccacagt ttgcggatga caaaccacat tcggccattt
     5161 acgccttaga cgtaatttgc attaagtttt tcggcatgga cttgacaagc ggactgtttt
     5221 ctaaacagag catcccacta acgtaccatc ccgccgattc agcgaggccg gtagctcatt
     5281 gggacaacag cccaggaacc cgcaagtatg ggtacgatca cgccattgcc gccgaactct
     5341 cccgtagatt tccggtgttc cagctagctg ggaagggcac acaacttgat ttgcagacgg
     5401 ggagaaccag agttatctct gcacagcata acctggtccc ggtgaaccgc aatcttcctc
     5461 acgccttagt ccccgagtac aaggagaagc aacccggccc ggtcgaaaaa ttcttgaacc
     5521 agttcaaaca ccactcagta cttgtggtat cagaggaaaa aattgaagct ccccgtaaga
     5581 gaatcgaatg gatcgccccg attggcatag ccggtgcaga taagaactac aacctggctt
     5641 tcgggtttcc gccgcaggca cggtacgacc tggtgttcat caacattgga actaaataca
     5701 gaaaccacca ctttcagcag tgcgaagacc atgcggcgac cttaaaaacc ctttcgcgtt
     5761 cggccctgaa ttgccttaac ccaggaggca ccctcgtggt gaagtcctat ggctacgccg
     5821 accgcaacag tgaggacgta gtcaccgctc ttgccagaaa gtttgtcagg gtgtctgcag
     5881 cgagaccaga ttgtgtctca agcaatacag aaatgtacct gattttccga caactagaca
     5941 acagccgtac acggcaattc accccgcacc atctgaattg cgtgatttcg tccgtgtatg
     6001 agggtacaag agatggagtt ggagccgcgc cgtcataccg caccaaaagg gagaatattg
     6061 ctgactgtca agaggaagca gttgtcaacg cagccaatcc gctgggtaga ccaggcgaag
     6121 gagtctgccg tgccatctat aaacgttggc cgaccagttt taccgattca gccacggaga
     6181 caggcaccgc aagaatgact gtgtgcctag gaaagaaagt gatccacgcg gtcggccctg
     6241 atttccggaa gcacccagaa gcagaagcct tgaaattgct acaaaacgcc taccatgcag
     6301 tggcagactt agtaaatgaa cataacatca agtctgtcgc cattccactg ctatctacag
     6361 gcatttacgc agccggaaaa gaccgccttg aagtatcact taactgcttg acaaccgcgc
     6421 tagacagaac tgacgcggac gtaaccatct attgcctgga taagaagtgg aaggaaagaa
     6481 tcgacgcggc actccaactt aaggagtctg taacagagct gaaggatgaa gatatggaga
     6541 tcgacgatga gttagtatgg attcatccag acagttgctt gaagggaaga aagggattca
     6601 gtactacaaa aggaaaattg tattcgtact tcgaaggcac caaattccat caagcagcaa
     6661 aagacatggc ggagataaag gtcctgttcc ctaatgacca ggaaagtaat gaacaactgt
     6721 gtgcctacat attgggtgag accatggaag caatccgcga aaagtgcccg gtcgaccata
     6781 acccgtcgtc tagcccgccc aaaacgttgc cgtgcctttg catgtatgcc atgacgccag
     6841 aaagggtcca cagacttaga agcaataacg tcaaagaagt tacagtatgc tcctccaccc
     6901 cccttcctaa gcacaaaatt aagaatgttc agaaggttca gtgcacgaaa gtagtcctgt
     6961 ttaatccgca cactcccgca ttcgttcccg cccgtaagta catagaagtg ccagaacagc
     7021 ctaccgctcc tcctgcacag gccgaggagg cccccgaagt tgtagcgaca ccgtcaccat
     7081 ctacagctga taacacctcg cttgatgtca cagacatctc actggatatg gatgacagta
     7141 gcgaaggctc acttttttcg agctttagcg gatcggacaa ctctattact agtatggaca
     7201 gttggtcgtc aggacctagt tcactagaga tagtagaccg aaggcaggtg gtggtggctg
     7261 acgttcatgc cgtccaagag cctgccccta ttccaccgcc aaggctaaag aagatggccc
     7321 gcctggcagc ggcaagaaaa gagcccactc caccggcaag caatagctct gagtccctcc
     7381 acctctcttt tggtggggta tccatgtccc tcggatcaat tttcgacgga gagacggccc
     7441 gccaggcagc ggtacaaccc ctggcaacag gccccacgga tgtgcctatg tctttcggat
     7501 cgttttccga cggagagatt gatgagctga gccgcagagt aactgagtcc gaacccgtcc
     7561 tgtttggatc atttgaaccg ggcgaagtga actcaattat atcgtcccga tcagccgtat
     7621 cttttcctct acgcaagcag agacgtagac gcaggagcag gaggactgaa tactgactaa
     7681 ccggggtagg tgggtacata ttttcgacgg acacaggccc tgggcacttg caaaagaagt
     7741 ccgttctgca gaaccagctt acagaaccga ccttggagcg caatgtcctg gaaagaattc
     7801 atgccccggt gctcgacacg tcgaaagagg aacaactcaa actcaggtac cagatgatgc
     7861 ccaccgaagc caacaaaagt aggtaccagt ctcgtaaagt agaaaatcag aaagccataa
     7921 ccactgagcg actactgtca ggactacgac tgtataactc tgccacagat cagccagaat
     7981 gctataagat cacctatccg aaaccattgt actccagtag cgtaccggcg aactactccg
     8041 atccacagtt cgctgtagct gtctgtaaca actatctgca tgagaactat ccgacagtag
     8101 catcttatca gattactgac gagtacgatg cttacttgga tatggtagac gggacagtcg
     8161 cctgcctgga tactgcaacc ttctgccccg ctaagcttag aagttacccg aaaaaacatg
     8221 agtatagagc cccgaatatc cgcagtgcgg ttccatcagc gatgcagaac acgctacaaa
     8281 atgtgctcat tgccgcaact aaaagaaatt gcaacgtcac gcagatgcgt gaactgccaa
     8341 cactggactc agcgacattc aatgtcgaat gctttcgaaa atatgcatgt aatgacgagt
     8401 attgggagga gttcgctcgg aagccaatta ggattaccac tgagtttgtc accgcatatg
     8461 tagctagact gaaaggccct aaggccgccg cactatttgc aaagacgtat aatttggtcc
     8521 cattgcaaga agtgcctatg gatagattcg tcatggacat gaaaagagac gtgaaagtta
     8581 caccaggcac gaaacacaca gaagaaagac cgaaagtaca agtgatacaa gccgcagaac
     8641 ccctggcgac tgcttactta tgcgggattc accgggaatt agtgcgtagg cttacggccg
     8701 tcttgcttcc aaacattcac acgctttttg acatgtcggc ggaggatttt gatgcaatca
     8761 tagcagaaca cttcaagcaa ggcgacccgg tactggagac ggatatcgca tcattcgaca
     8821 aaagccaaga cgacgctatg gcgttaaccg gtctgatgat cttggaggac ctgggtgtgg
     8881 atcaaccact actcgacttg atcgagtgcg cctttggaga aatatcatcc acccatctac
     8941 ctacgggtac tcgttttaaa ttcggggcga tgatgaaatc cggaatgttc ctcacacttt
     9001 ttgtcaacac agttttgaat gtcgttatcg ccagcagagt actagaagag cggcttaaaa
     9061 cgtccagatg tgcagcgttc attggcgacg acaacatcat acatggagta gtatctgaca
     9121 aagaaatggc tgagaggtgc gccacctggc tcaacatgga ggttaagatc atcgacgcag
     9181 tcatcggtga gagaccacct tacttctgcg gcggatttat cttgcaagat tcggttactt
     9241 ccacagcgtg ccgcgtggcg gatcccctga aaaggctgtt taagttgggt aaaccgctcc
     9301 cagccgacga cgagcaagac gaagacagaa gacgcgctct gctagatgaa acaaaggcgt
     9361 ggtttagagt aggtataaca ggcactttag cagtggccgt gacgacccgg tatgaggtag
     9421 acaatattac acctgtccta ctggcattga gaacttttgc ccagagcaaa agagcattcc
     9481 aagccatcag aggggaaata aagcatctct acggtggtcc taaatagtca gcatagtaca
     9541 tttcatctga ctaataccac aacaccacca cctctagagc caccatggca cttcccagat
     9601 gcatgtggcc aaattatgtt tggagagcta tgatggcatg tgtggtccac aggggatccg
     9661 gtgccccatt gactctctgc ttgttgggat gtttgctaca gacttttcac gtactctctc
     9721 aaaagtatcc atacgacgtt ccggactacg cagaattcat ggacaaagac tgcgaaatga
     9781 agcgcaccac cctggatagc cctctgggca agctggaact gtctgggtgc gaacagggcc
     9841 tgcaccgtat catcttcctg ggcaaaggaa catctgccgc cgacgccgtg gaagtgcctg
     9901 ccccagccgc cgtgctgggc ggaccagagc cactgatgca ggccaccgcc tggctcaacg
     9961 cctactttca ccagcctgag gccatcgagg agttccctgt gccagccctg caccacccag
    10021 tgttccagca ggagagcttt acccgccagg tgctgtggaa actgctgaaa gtggtgaagt
    10081 tcggagaggt catcagctac agccacctgg ccgccctggc cggcaatccc gccgccaccg
    10141 ccgccgtgaa aaccgccctg agcggaaatc ccgtgcccat tctgatcccc tgccaccggg
    10201 tggtgcaggg cgacctggac gtggggggct acgagggcgg gctcgccgtg aaagagtggc
    10261 tgctggccca cgagggccac agactgggca agcctgggct gggtgtcgac ttggatgatg
    10321 tagacccatt ggttactact aactttggca agattagggg aattaagaaa gaactcaata
    10381 atgaaatttt gggtcctgtc attcagtttc ttggggttcc atatgccgct ccaccaacag
    10441 gagaacatcg tttccagcct ccagaaccac catctccctg gtctgacatc cggaacgcca
    10501 ctcagtttgc tcctgtatgt ccccagaata tcattgatgg cagattgcct gaagttatgc
    10561 ttcctgtgtg gttcactaat aacttggatg tggtttcatc atacgtccaa gaccagagtg
    10621 aagactgtct atacttaaac atctatgtcc caactgaaga tggtcccctt acaaagaaac
    10681 acacagatga tttaggtgat aatgacggtg ctgaagatga agatattcgg gacagtgggg
    10741 gtcccaaacc agtgatggtg tacatccatg gcggctctta catggaaggt actggaaatc
    10801 tgtatgatgg gagtgtcttg gcaagctatg gcaatgtgat cgtcatcaca gtcaactatc
    10861 ggcttggggt acttggcttc ttgagcacag gggatcaggc tgccaaagga aactacgggc
    10921 tccttgacct catccaggcc ctaagatgga ccagcgagaa cattgggttc tttggtggtg
    10981 accccttgcg aatcactgtg tttggatcag gcgctggggg ttcatgtgtc aacctgctga
    11041 ctttatccca ttattctgaa ggtaaccgtt ggagcaattc aaccaaagga ctttttcaac
    11101 gagcaatagc tcagagtgga acagcccttt ccagctgggc tgttagtttc cagcctgcaa
    11161 aatacgctag aattctggcc acaaaagttg gctgcaatgt ttcagataca gtagagttag
    11221 tagaatgcct gcagaagaag ccttacaaag aacttgttga tcaagatgtt caaccagccc
    11281 gataccacat agcctttgga cctgtgatcg atggtgatgt aataccagat gaccctcaga
    11341 tactgatgga acaaggagag ttcctcaact atgatataat gttgggagtt aaccaagggg
    11401 aagggttgaa gtttgtcgaa aatatagtag atagtgatga tggtgtatca gccagtgatt
    11461 tcgactttgc tgtttctaat tttgttgata atttatatgg atatcctgaa ggcaaagatg
    11521 ttttgagaga aaccattaaa ttcatgtata ctgactgggc tgatcgccat aatcctgaaa
    11581 ctagaaggaa gacattgttg gctttgttta cggaccatca atgggtagca cctgctgtgg
    11641 ccacagcaga ccttcactcg aactttggct cacctacata cttctatgcc ttttatcatc
    11701 attgccaaac agatcaagtt ccagcttggg ctgatgcagc tcatggggat gaggttccct
    11761 atgtgttggg aatccccatg attggcccta cagagttatt tccttgcaat ttctccaaga
    11821 atgatgtgat gttgagtgca gtagtaatga catactggac gaattttgct aaaactggtg
    11881 acccaaatca accagttcct caagacacaa aattcatcca taccaaaccc aaccgctttg
    11941 aagaagtagc atggaccaga tattcccaga aagaccagct ttatctccat attggattaa
    12001 aaccgagagt taaagagcat tacagagcca ataaggtaaa tctctggctg gagctggtac
    12061 ctcatctgca taatctcaat gacatttctc agtatacctc gacaacaact aaagtgccat
    12121 ccacggacat cactctcaga cctacaagga agaattccac accagtcaca tcagcctttc
    12181 ccactgccaa acaggatgat cccaagcaac aaccaagccc cttctcggtg gatcagaggg
    12241 actactccac agagctaagt gtcactatcg cagtgggggc ctctctgctg tttctcaaca
    12301 tcttggcttt tgcagccctg tactacaaga aggataagag gagacatgat gtccaccgga
    12361 ggtgcagccc tcagcgcacg accaccaacg acctaaccca tgctccagaa gaggaaatta
    12421 tgtctctcca aatgaagcac actgacttgg atcacgagtg tgagtccatc catccacatg
    12481 aggtggttct tcggaccagc gctccaccgc tcgacggagc cggagctggc gctggagctg
    12541 gagccggagc tggcgggcta gccaccatgg acgcacaaac acgacgacgt gagcgtcgcg
    12601 ctgagaaaca agctcaatgg aaagctgcaa acctcgagcc accgctcgac ggagccggag
    12661 ctggcgctgg agctggagcc ggagctggcg gtctagccac cccaccgagc gctgcctgtc
    12721 ccccagatta tactctagct atgaggaggt cacctgatga tattccacta atgacaccta
    12781 acaccatcac aatgattccc aacactatac cagggattca gcccttacat acattcaaca
    12841 catttactgg aggacagaat aatacactgc cccatcccca cccacacccc cattcacatt
    12901 caacaaccag ggtataggca tgcaggcctt aaagcatctc tacggtggtc ctaaatagtc
    12961 agcatagtac atttcatctg actaatacca caacaccacc acctctagta gaggcgcgcc
    13021 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
    13081 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
    13141 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
    13201 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
    13261 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
    13321 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
    13381 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
    13441 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
    13501 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
    13561 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
    13621 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
    13681 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtcttagagg
    13741 ggcccaatga tccgaccagc aaaactcgat gtacttccga ggaactgatg tgcataatgc
    13801 atcaggctgg tacattagat ccccgcttac cgcgggcaat atagcaacac taaaaactcg
    13861 atgtacttcc gaggaagcgc agtgcataat gctgcgcagt gttgccacat aaccactata
    13921 ttaaccattt atctagcgga cgccaaaaac tcaatgtatt t
//
