LOCUS       sequence-173034-       13273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic Sindbis virus plasmid for expression of SYNseq components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 13273)
  AUTHORS   Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, 
            Correa IR Jr, Bressan D, Zador AM
  TITLE     Using high-throughput barcode sequencing to efficiently map 
            connectomes.
  JOURNAL   Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292.
  PUBMED    28449067
REFERENCE   2  (bases 1 to 13273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2017-07-7"; volume: "45(12):e115. doi"; pages: "
            10.1093/nar/gkx292"
COMMENT     Sequence Label: IDP466
FEATURES             Location/Qualifiers
     source          1..13273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8..10,11..13,14..727)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8..10,11..13,14..724)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        1345..1449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1450..1518,1519..2310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1668..1687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2481..3069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2970..2989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        3157..3175
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3157..3174
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             10977..11006
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             11019..11084
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11091..11636
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylcytosine 
                     derivatives"
                     /label=CLIP-tag(TM)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      12753..12762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             12759..12824
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
ORIGIN
        1 tgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      481 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
      721 caagtaaaga tcttacctaa ttgccgtcgt gaggtacgac caccgctagc tgtacagccc
      781 tgaaaaaggg ctcgagccct gaaaaagggc aattgccctg aaaaagggcg tcgacgccct
      841 gaaaaagggc ggaattcatg tccaatttac tgactgtaca ccaaaatttg cctgctaccg
      901 cgggcccaat gatccgacca gcaaaactcg atgtacttcc gaggaactga tgtgcataat
      961 gcatcaggct ggtacattag atccccgctt accgcgggca atatagcaac actaaaaact
     1021 cgatgtactt ccgaggaagc gcagtgcata atgctgcgca gtgttgccac ataaccacta
     1081 tattaaccat ttatctagcg gacgccaaaa actcaatgta tttctgagga agcgtggtgc
     1141 ataatgccac gcagcgtctg cataactttt attatttctt ttattaatca acaaaatttt
     1201 gtttttaaca tttcaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaagg ggaattcctc
     1261 gataattaag cgaccgctta attaatcgag gggaattaat tcttgaagac gaaagggcca
     1321 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     1381 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     1441 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     1501 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     1561 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     1621 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     1681 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     1741 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     1801 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     1861 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     1921 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     1981 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     2041 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     2101 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     2161 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     2221 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     2281 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     2341 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     2401 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     2461 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     2521 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     2581 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     2641 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     2701 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     2761 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     2821 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     2881 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     2941 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3001 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3061 ctatggaaaa acgccagcaa cgcgagctcg tatggacata ttgtcgttag aacgcggcta
     3121 caattaatac ataaccttat gtatcataca catacgattt aggtgacact atagattgac
     3181 ggcgtagtac acactattga atcaaacagc cgaccaattg cactaccatc acaatggaga
     3241 agccagtagt aaacgtagac gtagaccccc agagtccgtt tgtcgtgcaa ctgcaaaaaa
     3301 gcttcccgca atttgaggta gtagcacagc aggtcactcc aaatgaccat gctaatgcca
     3361 gagcattttc gcatctggcc agtaaactaa tcgagctgga ggttcctacc acagcgacga
     3421 tcttggacat aggcagcgca ccggctcgta gaatgttttc cgagcaccag tatcattgtg
     3481 tctgccccat gcgtagtcca gaagacccgg accgcatgat gaaatatgcc agtaaactgg
     3541 cggaaaaagc gtgcaagatt acaaacaaga acttgcatga gaagattaag gatctccgga
     3601 ccgtacttga tacgccggat gctgaaacac catcgctctg ctttcacaac gatgttacct
     3661 gcaacatgcg tgccgaatat tccgtcatgc aggacgtgta tatcaacgct cccggaacta
     3721 tctatcatca ggctatgaaa ggcgtgcgga ccctgtactg gattggcttc gacaccaccc
     3781 agttcatgtt ctcggctatg gcaggttcgt accctgcgta caacaccaac tgggccgacg
     3841 agaaagtcct tgaagcgcgt aacatcggac tttgcagcac aaagctgagt gaaggtagga
     3901 caggaaaatt gtcgataatg aggaagaagg agttgaagcc cgggtcgcgg gtttatttct
     3961 ccgtaggatc gacactttat ccagaacaca gagccagctt gcagagctgg catcttccat
     4021 cggtgttcca cttgaatgga aagcagtcgt acacttgccg ctgtgataca gtggtgagtt
     4081 gcgaaggcta cgtagtgaag aaaatcacca tcagtcccgg gatcacggga gaaaccgtgg
     4141 gatacgcggt tacacacaat agcgagggct tcttgctatg caaagttact gacacagtaa
     4201 aaggagaacg ggtatcgttc cctgtgtgca cgtacatccc ggccaccata tgcgatcaga
     4261 tgactggtat aatggccacg gatatatcac ctgacgatgc acaaaaactt ctggttgggc
     4321 tcaaccagcg aattgtcatt aacggtagga ctaacaggaa caccaacacc atgcaaaatt
     4381 accttctgcc gatcatagca caagggttca gcaaatgggc taaggagcgc aaggatgatc
     4441 ttgataacga gaaaatgctg ggtactagag aacgcaagct tacgtatggc tgcttgtggg
     4501 cgtttcgcac taagaaagta cattcgtttt atcgcccacc tggaacgcag acctgcgtaa
     4561 aagtcccagc ctcttttagc gcttttccca tgtcgtccgt atggacgacc tctttgccca
     4621 tgtcgctgag gcagaaattg aaactggcat tgcaaccaaa gaaggaggaa aaactgctgc
     4681 aggtctcgga ggaattagtc atggaggcca aggctgcttt tgaggatgct caggaggaag
     4741 ccagagcgga gaagctccga gaagcacttc caccattagt ggcagacaaa ggcatcgagg
     4801 cagccgcaga agttgtctgc gaagtggagg ggctccaggc ggacatcgga gcagcattag
     4861 ttgaaacccc gcgcggtcac gtaaggataa tacctcaagc aaatgaccgt atgatcggac
     4921 agtatatcgt tgtctcgcca aactctgtgc tgaagaatgc caaactcgca ccagcgcacc
     4981 cgctagcaga tcaggttaag atcataacac actccggaag atcaggaagg tacgcggtcg
     5041 aaccatacga cgctaaagta ctgatgccag caggaggtgc cgtaccatgg ccagaattcc
     5101 tagcactgag tgagagcgcc acgttagtgt acaacgaaag agagtttgtg aaccgcaaac
     5161 tataccacat tgccatgcat ggccccgcca agaatacaga agaggagcag tacaaggtta
     5221 caaaggcaga gcttgcagaa acagagtacg tgtttgacgt ggacaagaag cgttgcgtta
     5281 agaaggaaga agcctcaggt ctggtcctct cgggagaact gaccaaccct ccctatcatg
     5341 agctagctct ggagggactg aagacccgac ctgcggtccc gtacaaggtc gaaacaatag
     5401 gagtgatagg cacaccgggg tcgggcaagt cagctattat caagtcaact gtcacggcac
     5461 gagatcttgt taccagcgga aagaaagaaa attgtcgcga aattgaggcc gacgtgctaa
     5521 gactgagggg tatgcagatt acgtcgaaga cagtagattc ggttatgctc aacggatgcc
     5581 acaaagccgt agaagtgctg tacgttgacg aagcgttcgc gtgccacgca ggagcactac
     5641 ttgccttgat tgctatcgtc aggccccgca agaaggtagt actatgcgga gaccccatgc
     5701 aatgcggatt cttcaacatg atgcaactaa aggtacattt caatcaccct gaaaaagaca
     5761 tatgcaccaa gacattctac aagtatatct cccggcgttg cacacagcca gttacagcta
     5821 ttgtatcgac actgcattac gatggaaaga tgaaaaccac gaacccgtgc aagaagaaca
     5881 ttgaaatcga tattacaggg gccacaaagc cgaagccagg ggatatcatc ctgacatgtt
     5941 tccgcgggtg ggttaagcaa ttgcaaatcg actatcccgg acatgaagta atgacagccg
     6001 cggcctcaca agggctaacc agaaaaggag tgtatgccgt ccggcaaaaa gtcaatgaaa
     6061 acccactgta cgcgatcaca tcagagcatg tgaacgtgtt gctcacccgc actgaggaca
     6121 ggctagtgtg gaaaaccttg cagggcgacc catggattaa gcagctcact aacataccta
     6181 aaggaaactt tcaggctact atagaggact gggaagctga acacaaggga ataattgctg
     6241 caataaacag ccccactccc cgtgccaatc cgttcagctg caagaccaac gtttgctggg
     6301 cgaaagcatt ggaaccgata ctagccacgg ccggtatcgt acttaccggt tgccagtgga
     6361 gcgaactgtt cccacagttt gcggatgaca aaccacattc ggccatttac gccttagacg
     6421 taatttgcat taagtttttc ggcatggact tgacaagcgg actgttttct aaacagagca
     6481 tcccactaac gtaccatccc gccgattcag cgaggccggt agctcattgg gacaacagcc
     6541 caggaacccg caagtatggg tacgatcacg ccattgccgc cgaactctcc cgtagatttc
     6601 cggtgttcca gctagctggg aagggcacac aacttgattt gcagacgggg agaaccagag
     6661 ttatctctgc acagcataac ctggtcccgg tgaaccgcaa tcttcctcac gccttagtcc
     6721 ccgagtacaa ggagaagcaa cccggcccgg tcgaaaaatt cttgaaccag ttcaaacacc
     6781 actcagtact tgtggtatca gaggaaaaaa ttgaagctcc ccgtaagaga atcgaatgga
     6841 tcgccccgat tggcatagcc ggtgcagata agaactacaa cctggctttc gggtttccgc
     6901 cgcaggcacg gtacgacctg gtgttcatca acattggaac taaatacaga aaccaccact
     6961 ttcagcagtg cgaagaccat gcggcgacct taaaaaccct ttcgcgttcg gccctgaatt
     7021 gccttaaccc aggaggcacc ctcgtggtga agtcctatgg ctacgccgac cgcaacagtg
     7081 aggacgtagt caccgctctt gccagaaagt ttgtcagggt gtctgcagcg agaccagatt
     7141 gtgtctcaag caatacagaa atgtacctga ttttccgaca actagacaac agccgtacac
     7201 ggcaattcac cccgcaccat ctgaattgcg tgatttcgtc cgtgtatgag ggtacaagag
     7261 atggagttgg agccgcgccg tcataccgca ccaaaaggga gaatattgct gactgtcaag
     7321 aggaagcagt tgtcaacgca gccaatccgc tgggtagacc aggcgaagga gtctgccgtg
     7381 ccatctataa acgttggccg accagtttta ccgattcagc cacggagaca ggcaccgcaa
     7441 gaatgactgt gtgcctagga aagaaagtga tccacgcggt cggccctgat ttccggaagc
     7501 acccagaagc agaagccttg aaattgctac aaaacgccta ccatgcagtg gcagacttag
     7561 taaatgaaca taacatcaag tctgtcgcca ttccactgct atctacaggc atttacgcag
     7621 ccggaaaaga ccgccttgaa gtatcactta actgcttgac aaccgcgcta gacagaactg
     7681 acgcggacgt aaccatctat tgcctggata agaagtggaa ggaaagaatc gacgcggcac
     7741 tccaacttaa ggagtctgta acagagctga aggatgaaga tatggagatc gacgatgagt
     7801 tagtatggat tcatccagac agttgcttga agggaagaaa gggattcagt actacaaaag
     7861 gaaaattgta ttcgtacttc gaaggcacca aattccatca agcagcaaaa gacatggcgg
     7921 agataaaggt cctgttccct aatgaccagg aaagtaatga acaactgtgt gcctacatat
     7981 tgggtgagac catggaagca atccgcgaaa agtgcccggt cgaccataac ccgtcgtcta
     8041 gcccgcccaa aacgttgccg tgcctttgca tgtatgccat gacgccagaa agggtccaca
     8101 gacttagaag caataacgtc aaagaagtta cagtatgctc ctccaccccc cttcctaagc
     8161 acaaaattaa gaatgttcag aaggttcagt gcacgaaagt agtcctgttt aatccgcaca
     8221 ctcccgcatt cgttcccgcc cgtaagtaca tagaagtgcc agaacagcct accgctcctc
     8281 ctgcacaggc cgaggaggcc cccgaagttg tagcgacacc gtcaccatct acagctgata
     8341 acacctcgct tgatgtcaca gacatctcac tggatatgga tgacagtagc gaaggctcac
     8401 ttttttcgag ctttagcgga tcggacaact ctattactag tatggacagt tggtcgtcag
     8461 gacctagttc actagagata gtagaccgaa ggcaggtggt ggtggctgac gttcatgccg
     8521 tccaagagcc tgcccctatt ccaccgccaa ggctaaagaa gatggcccgc ctggcagcgg
     8581 caagaaaaga gcccactcca ccggcaagca atagctctga gtccctccac ctctcttttg
     8641 gtggggtatc catgtccctc ggatcaattt tcgacggaga gacggcccgc caggcagcgg
     8701 tacaacccct ggcaacaggc cccacggatg tgcctatgtc tttcggatcg ttttccgacg
     8761 gagagattga tgagctgagc cgcagagtaa ctgagtccga acccgtcctg tttggatcat
     8821 ttgaaccggg cgaagtgaac tcaattatat cgtcccgatc agccgtatct tttcctctac
     8881 gcaagcagag acgtagacgc aggagcagga ggactgaata ctgactaacc ggggtaggtg
     8941 ggtacatatt ttcgacggac acaggccctg ggcacttgca aaagaagtcc gttctgcaga
     9001 accagcttac agaaccgacc ttggagcgca atgtcctgga aagaattcat gccccggtgc
     9061 tcgacacgtc gaaagaggaa caactcaaac tcaggtacca gatgatgccc accgaagcca
     9121 acaaaagtag gtaccagtct cgtaaagtag aaaatcagaa agccataacc actgagcgac
     9181 tactgtcagg actacgactg tataactctg ccacagatca gccagaatgc tataagatca
     9241 cctatccgaa accattgtac tccagtagcg taccggcgaa ctactccgat ccacagttcg
     9301 ctgtagctgt ctgtaacaac tatctgcatg agaactatcc gacagtagca tcttatcaga
     9361 ttactgacga gtacgatgct tacttggata tggtagacgg gacagtcgcc tgcctggata
     9421 ctgcaacctt ctgccccgct aagcttagaa gttacccgaa aaaacatgag tatagagccc
     9481 cgaatatccg cagtgcggtt ccatcagcga tgcagaacac gctacaaaat gtgctcattg
     9541 ccgcaactaa aagaaattgc aacgtcacgc agatgcgtga actgccaaca ctggactcag
     9601 cgacattcaa tgtcgaatgc tttcgaaaat atgcatgtaa tgacgagtat tgggaggagt
     9661 tcgctcggaa gccaattagg attaccactg agtttgtcac cgcatatgta gctagactga
     9721 aaggccctaa ggccgccgca ctatttgcaa agacgtataa tttggtccca ttgcaagaag
     9781 tgcctatgga tagattcgtc atggacatga aaagagacgt gaaagttaca ccaggcacga
     9841 aacacacaga agaaagaccg aaagtacaag tgatacaagc cgcagaaccc ctggcgactg
     9901 cttacttatg cgggattcac cgggaattag tgcgtaggct tacggccgtc ttgcttccaa
     9961 acattcacac gctttttgac atgtcggcgg aggattttga tgcaatcata gcagaacact
    10021 tcaagcaagg cgacccggta ctggagacgg atatcgcatc attcgacaaa agccaagacg
    10081 acgctatggc gttaaccggt ctgatgatct tggaggacct gggtgtggat caaccactac
    10141 tcgacttgat cgagtgcgcc tttggagaaa tatcatccac ccatctacct acgggtactc
    10201 gttttaaatt cggggcgatg atgaaatccg gaatgttcct cacacttttt gtcaacacag
    10261 ttttgaatgt cgttatcgcc agcagagtac tagaagagcg gcttaaaacg tccagatgtg
    10321 cagcgttcat tggcgacgac aacatcatac atggagtagt atctgacaaa gaaatggctg
    10381 agaggtgcgc cacctggctc aacatggagg ttaagatcat cgacgcagtc atcggtgaga
    10441 gaccacctta cttctgcggc ggatttatct tgcaagattc ggttacttcc acagcgtgcc
    10501 gcgtggcgga tcccctgaaa aggctgttta agttgggtaa accgctccca gccgacgacg
    10561 agcaagacga agacagaaga cgcgctctgc tagatgaaac aaaggcgtgg tttagagtag
    10621 gtataacagg cactttagca gtggccgtga cgacccggta tgaggtagac aatattacac
    10681 ctgtcctact ggcattgaga acttttgccc agagcaaaag agcattccaa gccatcagag
    10741 gggaaataaa gcatctctac ggtggtccta aatagtcagc atagtacatt tcatctgact
    10801 aataccacaa caccaccacc tctagagcca ccatgtacca gaggatgctc cggtgcggcg
    10861 ccgatctggg atcgcccggg ggcggcagtg gcggcggcgc aggggggcgc ctggccctga
    10921 tctggatagt cccgctcacc ctcggcggcc tcctaggagt ggcctggggg gaattcgaac
    10981 aaaaactcat ctcagaagag gatctacaat tcggcggaga ctacaaagac catgacggtg
    11041 attataaaga tcatgacatc gattacaagg atgacgatga caagggagct atggacaaag
    11101 actgcgaaat gaagcgcacc accctggata gccctctggg caagctggaa ctgtctgggt
    11161 gcgaacaggg cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc gccgacgccg
    11221 tggaagtgcc tgccccagcc gccgtgctgg gcggaccaga gccactgatc caggccaccg
    11281 cctggctcaa cgcctacttt caccagcctg aggccatcga ggagttccct gtgccagccc
    11341 tgcaccaccc agtgttccag caggagagct ttacccgcca ggtgctgtgg aaactgctga
    11401 aagtggtgaa gttcggagag gtcatcagcg agagccacct ggccgccctg gtgggcaatc
    11461 ccgccgccac cgccgccgtg aacaccgccc tggacggaaa tcccgtgccc attctgatcc
    11521 cctgccaccg ggtggtgcag ggcgacagcg acgtggggcc ctacctgggc gggctcgccg
    11581 tgaaagagtg gctgctggcc cacgagggcc acagactggg caagcctggg ctgggtgtcg
    11641 acggtaccgc atccagtttg ggagcgcacc acatccacca tttccatggc agcagcaagc
    11701 atcattcagt gcctattgca atctacaggt caccagcatc cttgcgaggc ggacacgctg
    11761 ggacgacgta tatctttagc aaaggtggtg gacaaattac atataagtgg cctcctaatg
    11821 accgacccag tacacgagca gacaggctgg ccatcgggtt tagcactgtt cagaaggaag
    11881 cagtgttggt gcgtgtggac agttcctcag gcctgggtga ctaccttgag ctgcacatac
    11941 accaaggaaa aattggagtt aaatttaatg ttgggacaga tgacatcgcc attgaggagt
    12001 ccaatgcaat cattaatgat ggaaaatacc atgtagtgcg tttcacgagg agtggtggca
    12061 atgccacgtt acaggtggac agctggccag tcatcgaacg ataccctgca gggcgtcagc
    12121 tcacaatctt caatagccaa gcaaccataa taattggcgg gaaagagcag ggccagccct
    12181 tccagggcca gctctctggg ctttactaca atggcttgaa agttctgaat atggcggcag
    12241 agaacgatgc caacatcgcc atagtgggaa atgtgaggct ggtcggtgaa gtgccttcct
    12301 ctatgacaac tgagtcgaca gccactgcca tgcagtccga gatgtccacc tcaatcatgg
    12361 agaccaccac aaccctggct accagcacag ctcgacgagg aaagcccccc acaaaggagc
    12421 ctatcagcca gaccacggat gatatccttg tggcctcggc agagtgtccc agcgacgatg
    12481 aggacattga cccctgtgag ccgagctcag gtgggttagc caaccccacc cgagtgggcg
    12541 gacgcgaacc atacccaggc tcggcagagg tgatccggga gtctagcagt accactggca
    12601 tggtggtggg gattgtcgca gcagcagctc tgtgcatcct catcctcctc tatgccatgt
    12661 acaagtacag gaaccgggat gaagggtcga gcgctccacc gctcgacgga gccggagctg
    12721 gcgctggagc tggagccgga gctggcgggc tagccaccat ggacgcacaa acacgacgac
    12781 gtgagcgtcg cgctgagaaa caagctcaat ggaaagctgc aaacctcgag ccaccgctcg
    12841 acggagccgg agctggcgct ggagctggag ccggagctgg cggtctagcc accccaccga
    12901 gcgcttacca cgtggatgag agtcgaaact acatcagtaa ctcagcacag tccaatgggg
    12961 ctgtggtcaa ggagatgcaa cccagcagtg ctaaaagtgc caacaaaaac aagaagaaca
    13021 aggataagga gtattatgtc taggcatgca ggccttaaag catctctacg gtggtcctaa
    13081 atagtcagca tagtacattt catctgacta ataccacaac accaccacct ctagtagagg
    13141 cgccggacga tgccgtcctc gtagttcggg catgtactgg agccgagagg taacctcatt
    13201 ataatcgttc gctattcagg gattgaccaa caccggaaaa catctcactt gaagtaatat
    13261 atacgacaga gtc
//
