LOCUS       sequence-173036-       13281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic Sindbis virus plasmid for use in SYNseq PLA screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13281)
  AUTHORS   Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, 
            Correa IR Jr, Bressan D, Zador AM
  TITLE     Using high-throughput barcode sequencing to efficiently map 
            connectomes.
  JOURNAL   Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292.
  PUBMED    28449067
REFERENCE   2  (bases 1 to 13281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2017-07-7"; volume: "45(12):e115. doi"; pages: "
            10.1093/nar/gkx292"
COMMENT     Sequence Label: jk67
FEATURES             Location/Qualifiers
     source          1..13281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             7803..7832
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7839..8384
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylcytosine 
                     derivatives"
                     /label=CLIP-tag(TM)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      9501..9510
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9507..9572
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      9630..9639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9636..9701
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      9759..9768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9765..9830
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      9888..9897
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9894..9959
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      10285..10294
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10291..11004
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(10436..10454)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10704..10723
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        11452..11556
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11557..11625,11626..12417)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11775..11794)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12588..13176
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13077..13096
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(13264..13281,1)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     13264..13281
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
      121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
      301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagta
      361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
      421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
      481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
      541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
      721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
      781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
     1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
     1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
     1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
     1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
     1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
     1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
     1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
     1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
     1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
     1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
     1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
     1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
     1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
     2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
     2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
     2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
     2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
     2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
     2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
     2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
     2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
     2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
     2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
     2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
     2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag ctcactaaca
     3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
     3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
     3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
     3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
     3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
     3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
     3541 tagtccccga gtacaaggag aagcaacccg gcccggtcga aaaattcttg aaccagttca
     3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
     3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
     3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
     3841 tgaattgcct taacccagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
     3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
     3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
     4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
     4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
     4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
     4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
     4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
     4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
     4621 atgagttagt atggattcat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
     4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
     4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
     4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
     5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
     5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
     5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
     5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
     5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
     5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
     5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
     5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
     5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
     5701 ctctacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
     5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
     5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
     5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
     5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
     6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
     6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
     6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
     6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
     6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
     6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
     6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
     6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
     6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
     6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
     6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
     6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
     6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
     6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
     6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
     6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
     6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
     7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
     7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
     7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
     7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
     7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
     7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
     7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
     7561 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tacatttcat
     7621 ctgactaata ccacaacacc accacctcta gagccaccat gtaccagagg atgctccggt
     7681 gcggcgccga tctgggatcg cccgggggcg gcagtggcgg cggcgcaggg gggcgcctgg
     7741 ccctgatctg gatagtcccg ctcaccctcg gcggcctcct aggagtggcc tggggggaat
     7801 tcgaacaaaa actcatctca gaagaggatc tacaattcat ggacaaagac tgcgaaatga
     7861 agcgcaccac cctggatagc cctctgggca agctggaact gtctgggtgc gaacagggcc
     7921 tgcaccgtat catcttcctg ggcaaaggaa catctgccgc cgacgccgtg gaagtgcctg
     7981 ccccagccgc cgtgctgggc ggaccagagc cactgatcca ggccaccgcc tggctcaacg
     8041 cctactttca ccagcctgag gccatcgagg agttccctgt gccagccctg caccacccag
     8101 tgttccagca ggagagcttt acccgccagg tgctgtggaa actgctgaaa gtggtgaagt
     8161 tcggagaggt catcagcgag agccacctgg ccgccctggt gggcaatccc gccgccaccg
     8221 ccgccgtgaa caccgccctg gacggaaatc ccgtgcccat tctgatcccc tgccaccggg
     8281 tggtgcaggg cgacagcgac gtggggccct acctgggcgg gctcgccgtg aaagagtggc
     8341 tgctggccca cgagggccac agactgggca agcctgggct gggtgtcgac ggtaccgcat
     8401 ccagtttggg agcgcaccac atccaccatt tccatggcag cagcaagcat cattcagtgc
     8461 ctattgcaat ctacaggtca ccagcatcct tgcgaggcgg acacgctggg acgacgtata
     8521 tctttagcaa aggtggtgga caaattacat ataagtggcc tcctaatgac cgacccagta
     8581 cacgagcaga caggctggcc atcgggttta gcactgttca gaaggaagca gtgttggtgc
     8641 gtgtggacag ttcctcaggc ctgggtgact accttgagct gcacatacac caaggaaaaa
     8701 ttggagttaa atttaatgtt gggacagatg acatcgccat tgaggagtcc aatgcaatca
     8761 ttaatgatgg aaaataccat gtagtgcgtt tcacgaggag tggtggcaat gccacgttac
     8821 aggtggacag ctggccagtc atcgaacgat accctgcagg gcgtcagctc acaatcttca
     8881 atagccaagc aaccataata attggcggga aagagcaggg ccagcccttc cagggccagc
     8941 tctctgggct ttactacaat ggcttgaaag ttctgaatat ggcggcagag aacgatgcca
     9001 acatcgccat agtgggaaat gtgaggctgg tcggtgaagt gccttcctct atgacaactg
     9061 agtcgacagc cactgccatg cagtccgaga tgtccacctc aatcatggag accaccacaa
     9121 ccctggctac cagcacagct cgacgaggaa agccccccac aaaggagcct atcagccaga
     9181 ccacggatga tatccttgtg gcctcggcag agtgtcccag cgacgatgag gacattgacc
     9241 cctgtgagcc gagctcaggt gggttagcca accccacccg agtgggcggc cgcgaaccat
     9301 acccaggctc ggcagaggtg atccgggagt ctagcagtac cactggcatg gtggtgggga
     9361 ttgtcgcagc agcagctctg tgcatcctca tcctcctcta tgccatgtac aagtacagga
     9421 accgggatga agggtcgagc gctccaccgc tcgacggagc cggagctggc gctggagctg
     9481 gagccggagc tggcgggcta gccaccatgg acgcacaaac acgacgacgt gagcgtcgcg
     9541 ctgagaaaca agctcaatgg aaagctgcaa acccaccgct cgacggagcc ggagctggcg
     9601 ctggagctgg agccggagct ggcggtctag ccaccatgga cgcacaaaca cgacgacgtg
     9661 agcgtcgcgc tgagaaacaa gctcaatgga aagctgcaaa cccaccgctc gacggagccg
     9721 gagctggcgc tggagctgga gccggagctg gcggtctagc caccatggac gcacaaacac
     9781 gacgacgtga gcgtcgcgct gagaaacaag ctcaatggaa agctgcaaac ccaccgctcg
     9841 acggagccgg agctggcgct ggagctggag ccggagctgg cggtctagcc accatggacg
     9901 cacaaacacg acgacgtgag cgtcgcgctg agaaacaagc tcaatggaaa gctgcaaacc
     9961 caccgctcga gccaccgctc gacggagccg gagctggcgc tggagctgga gccggagctg
    10021 gcggtctagc caccccaccg agcgcttacc acgtggatga gagtcgaaac tacatcagta
    10081 actcagcaca gtccaatggg gctgtggtca aggagatgca acccagcagt gctaaaagcg
    10141 ccaacaaaaa caagaagaac aaggataagg agtattatgt ctaggcatgc aggccttaaa
    10201 gcatctctac ggtggtccta aatagtcagc atagtacatt tcatctgact aataccacaa
    10261 caccaccacc tctagtagag gcgcgccacc atggtgagca agggcgagga ggataacatg
    10321 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
    10381 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
    10441 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
    10501 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
    10561 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
    10621 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
    10681 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
    10741 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
    10801 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
    10861 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
    10921 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
    10981 atggacgagc tgtacaagtc ttagaggggc ccaatgatcc gaccagcaaa actcgatgta
    11041 cttccgagga actgatgtgc ataatgcatc aggctggtac attagatccc cgcttaccgc
    11101 gggcaatata gcaacactaa aaactcgatg tacttccgag gaagcgcagt gcataatgct
    11161 gcgcagtgtt gccacataac cactatatta accatttatc tagcggacgc caaaaactca
    11221 atgtatttct gaggaagcgt ggtgcataat gccacgcagc gtctgcataa cttttattat
    11281 ttcttttatt aatcaacaaa attttgtttt taacatttaa aaaaaaaaaa aaaaaaaaaa
    11341 aaaaaaaaaa aaanngggga attcctcgat aattaagcgg ccgcttaatt aatcgagggg
    11401 aattaattct tgaagacgaa agggccaggt ggcacttttc ggggaaatgt gcgcggaacc
    11461 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    11521 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    11581 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    11641 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    11701 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    11761 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
    11821 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    11881 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    11941 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    12001 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    12061 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    12121 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    12181 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    12241 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    12301 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    12361 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    12421 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    12481 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
    12541 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
    12601 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
    12661 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
    12721 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
    12781 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
    12841 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
    12901 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
    12961 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
    13021 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
    13081 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
    13141 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc gagctcgtat
    13201 ggacatattg tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat
    13261 acgatttagg tgacactata g
//
