LOCUS       sequence-173037-       10193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentivirus for expression of human Plexin-B2 with deletion of 
            ectodomain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Huang Y, Tejero R, Lee VK, Brusco C, Hannah T, Bertucci TB, 
            Junqueira Alves C, Katsyv I, Kluge M, Foty R, Zhang B, Friedel CC, 
            Dai G, Zou H, Friedel RH
  TITLE     Plexin-B2 facilitates glioblastoma infiltration by modulating cell 
            biomechanics.
  JOURNAL   Commun Biol. 2021 Jan 29;4(1):145. doi: 10.1038/s42003-021-01667-4.
  PUBMED    33514835
REFERENCE   2  (bases 1 to 10193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2021-01-29"; volume: "4(1):145. doi"; pages: " 
            10.1038/s42003-021-01667-4"
COMMENT     Sequence Label: pLV-PLXNB2-decto
FEATURES             Location/Qualifiers
     source          1..10193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..20)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     394..417
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    495..1083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             601..612
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     1010..1030
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    1114..1138
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3203..3227)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3282..3792)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3345..3363)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    3838..3955
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4222..4266)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4451..4684
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5177..5302
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5349..5529
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5530..5756)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(5784..5804)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5784..5802)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5823..5839)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5823..5839)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5836..5858)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5847..5863
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5871..5877,5878..5895,5896..5901))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5916..5937
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6054..6071)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6225..6813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6305..6324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6984..7775,7776..7844))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7607..7626
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7845..7949)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7975..8430)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8112..8133)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8324..8343
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8557..8579
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8571..8588
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8572..8588
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8598..8617
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8598..8616
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      8637..8772
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8812..8933
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8823..8842)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8877..8896
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             9005..9238
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(9329..10132)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9440..9459)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     10050..10069
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(join(10146..10193,1..452))
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
ORIGIN
        1 cttttgaagc gtgcagaatg ccgggcctcc ggaggacctt cgggcgcccg ccccgcccct
       61 gagcccgccc ctgagcccgc ccccggaccc accccttccc agcctctgag cccagaaagc
      121 gaaggagcaa agctgctatt ggccgctgcc ccaaaggcct acccgcttcc attgctcagc
      181 ggtgctgtcc atctgcacga gactagtgag acgtgctact tccatttgtc acgtcctgca
      241 cgacgcgagc tgcggggcgg gggggaactt cctgactagg ggaggagtag aaggtggcgc
      301 gaaggggcca ccaaagaacg gagccggttg gcgcctaccg gtggatgtgg aatgtgtgcg
      361 aggccagagg ccacttgtgt agcgccaagt gcccagcggg gctgctaaag cgcatgctcc
      421 agactgcctt gggaaaagcg cctcccctac ccggtagaat tagatcacta gtccggatat
      481 ctgcagaatt ccaggcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag
      541 gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg
      601 attgagggcc gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca
      661 ggcgagcagc catggaaagg acgtcagctt ccccgacaac accacggaat tgtcagtgcc
      721 caacagccga gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc
      781 aatagggagg gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca
      841 accagtgggg gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg
      901 ccacaactcc tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc
      961 catacgggaa gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa
     1021 aggagcaaca tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg
     1081 attgtcgagc ggccgccact gtgctggata tcaaccactt tgtacaagaa agctgggccc
     1141 atgaggccct agaggtcagt gaccttgttc tccagtgcag cggcaatctg ctgcaggcgg
     1201 aaggccagct gcatcttctg ggcggcagga tcctcctcca aggcattgat gatctcgtca
     1261 tagtacttct gcgtgtattg gtagagctgg tggagtgcca cgagggtgtt caaggagtcc
     1321 gtgtgcgccc gggaaatctc tgccaggtgt gtgttcatgt cctggtcgct gacctgcacc
     1381 atctgccgga tccccttgta gtaatcctcc accatcttct tgtaggtgga gatctccttg
     1441 gcgtacagca gcttgttgct gggagaatcg cggctcagct tatgctccgt gcgcgtgcag
     1501 gcatccatga aggtctgcgc gatgactgac agcgaggcgt ccaccacctc gtggacatgc
     1561 acgtcaaaga tgaagtgggg gttcttgagg atgttcaccc agaaccggag cggtaagctg
     1621 ttcgtcttcc agatgtggat ggtgtcttca tcctggatgt tgtgcttctc tgcctgctcg
     1681 tccaggaagt cgaagaagta cttgactgca ggtggcaccg cgtgcccagg cgccagcacg
     1741 ctctggaaga agttgtccac aaactgctgc agtgtgccct tgactgagag cagccgcgtc
     1801 aggtagatct cggtgatggc cttcgtccgc tccttctctt tcacgctgcc tctcttggac
     1861 ttgccctcgt ccacctcgtc ggtcggccgc accaggtgcc acacccggtt ctcctcctcc
     1921 aggagggcat ggcgctcccc aggcaggtcc tgctggctgt cctccggctg ctgggagacc
     1981 cccaccttgg acaggatgag ggtggctcca tcccggacat tgtagtgcat aagggtgttg
     2041 acgcgcttcc accggccctc ccgctgtgac gtcaggtcca ggtccgacag gatctgcgct
     2101 gtggagcccg gacgccactc caggaccacg ctgtctggcc tgggccagca ggagcagggc
     2161 tgcccacggt acacctggtc aatgatcttc tccttgacct gggagatggt gtcacagttg
     2221 aggaccttca ccgggatggc gtccactccc tcgtcctgca cgatcacgct caccgtcagg
     2281 ggtgcgtact ccacatcatc ccccagcagc cccgtgtcgt tgagagtgta cttggccttc
     2341 ttctgtaccg catccaccgg gcccttttcc acctgatgtt tgatggcctt gaagagcttg
     2401 tacaggggct ccccggcact gtccttgagg tactggtaca ggcagatgga catccagttg
     2461 gacagcatcc tctccaccac agtctcagac ctgcgcagca tcagcttggg gttcttggcc
     2521 accacgtact gctccaggag ctccaggaag agcgtgtgca tgatgtccgt gtagtactcc
     2581 agtttcccgt gcagcgccac cgtcagcagg gacgcgaagt agaccttggc gcgggccgag
     2641 aactcccgct ggttctccag ggtgtggatg aaattgatga ggaaagactt gctgttcagc
     2701 aggttggaga actggtagag ggcctgctcc accaccggcc gccgcggctc agggatgtcc
     2761 agcttgccgg tgatcatcac gtccttgtcg ccgtccttgg agggcaggaa gaagacgcgg
     2821 tcggtgtagg tcttgtagtc cagcacgggg atgccggcct cgtgcacgtc gttggtctgg
     2881 tcctccatct cgatcatcag gtctgtgaat tccttcttgc agcggtcccg cacgctctcc
     2941 tccaggccct ccagctggga cttgatcttc tcatactctc gttcggcctg ctggctcttc
     3001 ctccagtagc agtagacaga caccgcgatg acgaccacca tgggcacgat gaccagcggc
     3061 aagatgaggc tgagcggcac gtcgctcacc cgtgtgtcgc ggaagaagtc cagcttgcgg
     3121 ggcctcaggc tggcacctgc gcccagcagg cccagcaggg tcagggccca gagctgcagt
     3181 gccatggtgg gccttgacgg cccttctgct tttttgtaca aacttgttga tatctgcaga
     3241 attccaccac actggactag tcgacgaacc ggtggatccc cctggggaga gaggtcggtg
     3301 attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca gaatgcggaa
     3361 caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc ccgcaccgcc
     3421 ccttcccggc cgctgctctc ggcgcgccct gctgagcagc cgctattggc cacagcccat
     3481 cgcggtcggc gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt actagtgaga
     3541 cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc ttcccgactt
     3601 aggggcggag caggaagcgt cgccgggggg cccacaaggg tagcggcgaa gatccgggtg
     3661 acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt cggcgccgct gcgtttcccg
     3721 gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc
     3781 aaccccaacc ccgtggaatt cgatatcaag cttctcgagg ctagtctcgt gatcgataaa
     3841 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     3901 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaagttaa
     3961 ccgataccgt cgagatccgt tcactaatcg aatggatctg tctctgtctc tctctccacc
     4021 ttcttcttct attccttcgg gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa
     4081 cgataatggt gaatatccct gcctaactct attcactata gaaagtacag caaaaactat
     4141 tcttaaacct accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt
     4201 tgttatgtta aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc
     4261 attcttttct tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt
     4321 gttaatttct ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt
     4381 attactccaa ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc
     4441 ccaaatcccc aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg
     4501 gagctgcttg atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct
     4561 cagcaaattg ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag
     4621 cgtcattgac gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc
     4681 tcctattccc actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc
     4741 tactcctaat ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc
     4801 cctcatatct cctcctccag gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac
     4861 ttttgttttg ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc
     4921 tttgatgcac acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc
     4981 ctgtctgaag ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta
     5041 acaggccagg attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta
     5101 atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc
     5161 tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca
     5221 aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa
     5281 gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg
     5341 ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc
     5401 agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg
     5461 cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca
     5521 gagagacccg tttatgtatc gagctaggca cttaaataca atatctctgc aatgcggcaa
     5581 ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc cttcctaata aggcacgatc
     5641 gtaccacctt acttccacca atcggcatgc acggtgcttt ttctctcctt gtaaggcatg
     5701 ttgctaactc atcgttacca tgttgcaaga ctacaagagt attgcataag actacattaa
     5761 gcttgcagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     5821 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5881 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5941 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     6001 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     6061 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     6121 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6181 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6241 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6301 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6361 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     6421 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6481 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6541 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6601 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6661 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6721 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6781 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6841 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6901 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6961 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     7021 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     7081 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     7141 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7201 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7261 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7321 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7381 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7441 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7501 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7561 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7621 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7681 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7741 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7801 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7861 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7921 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     7981 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     8041 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     8101 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     8161 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     8221 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     8281 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     8341 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     8401 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     8461 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     8521 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     8581 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacg tccctaggcc
     8641 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     8701 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     8761 taggggcggg atgggcggag ttaggggcgg gatagctaga gccagacatg ataagataca
     8821 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     8881 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttcctctca
     8941 ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg acatgaacta
     9001 ctactgctag agattttcca cactgactaa aagggtctga gggatctcta gttaccagag
     9061 tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta
     9121 agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga
     9181 gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgagctag cccttccagt
     9241 cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt cattggtctt
     9301 aaaggtaccg ggccctcgac ggatcccctc agaagaactc gtcaagaagg cgatagaagg
     9361 cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt
     9421 cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg
     9481 ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat
     9541 tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct
     9601 tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct
     9661 gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt
     9721 ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga
     9781 tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc
     9841 ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa
     9901 cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac
     9961 cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg
    10021 cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc
    10081 aagcggccgg agaacctgcg tgcaatccat cttgttcaat ggccgatccc atattggctg
    10141 caggtcgaaa ggcccggaga tgaggaagag gagaacagcg cggcagacgt gcg
//
