LOCUS       sequence-173038-       14479 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic Sindbis virus plasmid for use in SYNseq PLA screen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14479)
  AUTHORS   Peikon ID, Kebschull JM, Vagin VV, Ravens DI, Sun YC, Brouzes E, 
            Correa IR Jr, Bressan D, Zador AM
  TITLE     Using high-throughput barcode sequencing to efficiently map 
            connectomes.
  JOURNAL   Nucleic Acids Res. 2017 Jul 7;45(12):e115. doi: 10.1093/nar/gkx292.
  PUBMED    28449067
REFERENCE   2  (bases 1 to 14479)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2017-07-7"; volume: "45(12):e115. doi"; pages: "
            10.1093/nar/gkx292"
COMMENT     Sequence Label: jk68
FEATURES             Location/Qualifiers
     source          1..14479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7653..7662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7800..7826
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7833..8378
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     regulatory      8840..8849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      10635..10644
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10641..10706
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      10764..10773
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10770..10835
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      10893..10902
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10899..10964
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      11022..11031
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11028..11093
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     regulatory      11485..11494
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11491..12204
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(11636..11654)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11904..11923
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        12650..12754
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12755..12823,12824..13615)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12973..12992)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13786..14374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14275..14294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(14462..14479,1)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     14462..14479
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
      121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
      301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagta
      361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
      421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
      481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
      541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
      721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
      781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
     1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
     1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
     1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
     1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
     1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
     1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
     1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
     1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
     1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
     1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
     1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
     1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
     1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
     2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
     2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
     2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
     2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
     2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
     2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
     2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
     2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
     2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
     2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
     2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
     2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag ctcactaaca
     3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
     3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
     3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
     3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
     3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
     3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
     3541 tagtccccga gtacaaggag aagcaacccg gcccggtcga aaaattcttg aaccagttca
     3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
     3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
     3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
     3841 tgaattgcct taacccagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
     3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
     3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
     4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
     4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
     4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
     4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
     4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
     4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
     4621 atgagttagt atggattcat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
     4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
     4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
     4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
     5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
     5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
     5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
     5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
     5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
     5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
     5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
     5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
     5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
     5701 ctctacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
     5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
     5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
     5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
     5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
     6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
     6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
     6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
     6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
     6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
     6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
     6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
     6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
     6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
     6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
     6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
     6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
     6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
     6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
     6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
     6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
     6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
     7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
     7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
     7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
     7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
     7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
     7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
     7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
     7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
     7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
     7561 tcagagggga aataaagcat ctctacggtg gtcctaaata gtcagcatag tacatttcat
     7621 ctgactaata ccacaacacc accacctcta gagccaccat ggcacttccc agatgcatgt
     7681 ggccaaatta tgtttggaga gctatgatgg catgtgtggt ccacagggga tccggtgccc
     7741 cattgactct ctgcttgttg ggatgtttgc tacagacttt tcacgtactc tctcaaaagt
     7801 atccatacga cgttccggac tacgcagaat tcatggacaa agactgcgaa atgaagcgca
     7861 ccaccctgga tagccctctg ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc
     7921 gtatcatctt cctgggcaaa ggaacatctg ccgccgacgc cgtggaagtg cctgccccag
     7981 ccgccgtgct gggcggacca gagccactga tgcaggccac cgcctggctc aacgcctact
     8041 ttcaccagcc tgaggccatc gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc
     8101 agcaggagag ctttacccgc caggtgctgt ggaaactgct gaaagtggtg aagttcggag
     8161 aggtcatcag ctacagccac ctggccgccc tggccggcaa tcccgccgcc accgccgccg
     8221 tgaaaaccgc cctgagcgga aatcccgtgc ccattctgat cccctgccac cgggtggtgc
     8281 agggcgacct ggacgtgggg ggctacgagg gcgggctcgc cgtgaaagag tggctgctgg
     8341 cccacgaggg ccacagactg ggcaagcctg ggctgggtgt cgacttggat gatgtagacc
     8401 cattggttac tactaacttt ggcaagatta ggggaattaa gaaagaactc aataatgaaa
     8461 ttttgggtcc tgtcattcag tttcttgggg ttccatatgc cgctccacca acaggagaac
     8521 atcgtttcca gcctccagaa ccaccatctc cctggtctga catccggaac gccactcagt
     8581 ttgctcctgt atgtccccag aatatcattg atggcagatt gcctgaagtt atgcttcctg
     8641 tgtggttcac taataacttg gatgtggttt catcatacgt ccaagaccag agtgaagact
     8701 gtctatactt aaacatctat gtcccaactg aagatggtcc ccttacaaag aaacacacag
     8761 atgatttagg tgataatgac ggtgctgaag atgaagatat tcgggacagt gggggtccca
     8821 aaccagtgat ggtgtacatc catggcggct cttacatgga aggtactgga aatctgtatg
     8881 atgggagtgt cttggcaagc tatggcaatg tgatcgtcat cacagtcaac tatcggcttg
     8941 gggtacttgg cttcttgagc acaggggatc aggctgccaa aggaaactac gggctccttg
     9001 acctcatcca ggccctaaga tggaccagcg agaacattgg gttctttggt ggtgacccct
     9061 tgcgaatcac tgtgtttgga tcaggcgctg ggggttcatg tgtcaacctg ctgactttat
     9121 cccattattc tgaaggtaac cgttggagca attcaaccaa aggacttttt caacgagcaa
     9181 tagctcagag tggaacagcc ctttccagct gggctgttag tttccagcct gcaaaatacg
     9241 ctagaattct ggccacaaaa gttggctgca atgtttcaga tacagtagag ttagtagaat
     9301 gcctgcagaa gaagccttac aaagaacttg ttgatcaaga tgttcaacca gcccgatacc
     9361 acatagcctt tggacctgtg atcgatggtg atgtaatacc agatgaccct cagatactga
     9421 tggaacaagg agagttcctc aactatgata taatgttggg agttaaccaa ggggaagggt
     9481 tgaagtttgt cgaaaatata gtagatagtg atgatggtgt atcagccagt gatttcgact
     9541 ttgctgtttc taattttgtt gataatttat atggatatcc tgaaggcaaa gatgttttga
     9601 gagaaaccat taaattcatg tatactgact gggctgatcg ccataatcct gaaactagaa
     9661 ggaagacatt gttggctttg tttacggacc atcaatgggt agcacctgct gtggccacag
     9721 cagaccttca ctcgaacttt ggctcaccta catacttcta tgccttttat catcattgcc
     9781 aaacagatca agttccagct tgggctgatg cagctcatgg ggatgaggtt ccctatgtgt
     9841 tgggaatccc catgattggc cctacagagt tatttccttg caatttctcc aagaatgatg
     9901 tgatgttgag tgcagtagta atgacatact ggacgaattt tgctaaaact ggtgacccaa
     9961 atcaaccagt tcctcaagac acaaaattca tccataccaa acccaaccgc tttgaagaag
    10021 tagcatggac cagatattcc cagaaagacc agctttatct ccatattgga ttaaaaccga
    10081 gagttaaaga gcattacaga gccaataagg taaatctctg gctggagctg gtacctcatc
    10141 tgcataatct caatgacatt tctcagtata cctcgacaac aactaaagtg ccatccacgg
    10201 acatcactct cagacctaca aggaagaatt ccacaccagt cacatcagcc tttcccactg
    10261 ccaaacagga tgatcccaag caacaaccaa gccccttctc ggtggatcag agggactact
    10321 ccacagagct aagtgtcact atcgcagtgg gggcctctct gctgtttctc aacatcttgg
    10381 cttttgcagc cctgtactac aagaaggata agaggagaca tgatgtccac cggaggtgca
    10441 gccctcagcg cacgaccacc aacgacctaa cccatgctcc agaagaggaa attatgtctc
    10501 tccaaatgaa gcacactgac ttggatcacg agtgtgagtc catccatcca catgaggtgg
    10561 ttcttcggac cagcgctcca ccgctcgacg gagccggagc tggcgctgga gctggagccg
    10621 gagctggcgg gctagccacc atggacgcac aaacacgacg acgtgagcgt cgcgctgaga
    10681 aacaagctca atggaaagct gcaaacccac cgctcgacgg agccggagct ggcgctggag
    10741 ctggagccgg agctggcggt ctagccacca tggacgcaca aacacgacga cgtgagcgtc
    10801 gcgctgagaa acaagctcaa tggaaagctg caaacccacc gctcgacgga gccggagctg
    10861 gcgctggagc tggagccgga gctggcggtc tagccaccat ggacgcacaa acacgacgac
    10921 gtgagcgtcg cgctgagaaa caagctcaat ggaaagctgc aaacccaccg ctcgacggag
    10981 ccggagctgg cgctggagct ggagccggag ctggcggtct agccaccatg gacgcacaaa
    11041 cacgacgacg tgagcgtcgc gctgagaaac aagctcaatg gaaagctgca aacccaccgc
    11101 tcgagccacc gctcgacgga gccggagctg gcgctggagc tggagccgga gctggcggtc
    11161 tagccacccc accgagcgct gcctgtcccc cagattatac tctagctatg aggaggtcac
    11221 ctgatgatat tccactaatg acacctaaca ccatcacaat gattcccaac actataccag
    11281 ggattcagcc cttacataca ttcaacacat ttactggagg acagaataat acactgcccc
    11341 atccccaccc acacccccat tcacattcaa caaccagggt ataggcatgc aggccttaaa
    11401 gcatctctac ggtggtccta aatagtcagc atagtacatt tcatctgact aataccacaa
    11461 caccaccacc tctagtagag gcgcgccacc atggtgagca agggcgagga ggataacatg
    11521 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
    11581 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
    11641 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
    11701 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
    11761 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
    11821 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
    11881 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
    11941 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
    12001 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
    12061 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
    12121 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
    12181 atggacgagc tgtacaagtc ttagaggggc ccaatgatcc gaccagcaaa actcgatgta
    12241 cttccgagga actgatgtgc ataatgcatc aggctggtac attagatccc cgcttaccgc
    12301 gggcaatata gcaacactaa aaactcgatg tacttccgag gaagcgcagt gcataatgct
    12361 gcgcagtgtt gccacataac cactatatta accatttatc tagcggacgc caaaaactca
    12421 atgtatttct gaggaagcgt ggtgcataat gccacgcagc gtctgcataa cttttattat
    12481 ttcttttatt aatcaacaaa attttgtttt taacatttca aaaaaaaaaa aaaaaaaaaa
    12541 aaaaaaaaaa aaaggggaat tcctcgataa ttaagcggcc gcttaattaa tcgaggggaa
    12601 ttaattcttg aagacgaaag ggccaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    12661 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    12721 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    12781 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    12841 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    12901 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    12961 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
    13021 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    13081 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    13141 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    13201 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    13261 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    13321 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    13381 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    13441 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
    13501 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    13561 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    13621 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    13681 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    13741 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    13801 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    13861 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    13921 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    13981 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    14041 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    14101 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    14161 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    14221 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    14281 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    14341 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcga gctcgtatgg
    14401 acatattgtc gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac
    14461 gatttaggtg acactatag
//
