LOCUS       sequence-17304-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Nallamsetty S, Kapust RB, Tozser J, Cherry S, Tropea JE, Copeland 
            TD, Waugh DS
  TITLE     Efficient site-specific processing of fusion proteins by tobacco 
            vein mottling virus protease in vivo and in vitro.
  JOURNAL   Protein Expr Purif. 2004 Nov . 38(1):108-15.
  PUBMED    15477088
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2004 Nov . 38(1):108-15."
COMMENT     Sequence Label: pHPK1212
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..720)
                     /codon_start=1
                     /product="tobacco vein mottling virus NIa protease 
                     (Nallamsetty et al., 2004)"
                     /label=TVMV protease
                     /translation="SKALLKGVRDFNPISACVCLLENSSDGHSERLFGIGFGPYIIANQ
                     HLFRRNNGELTIKTMHGEFKVKNSTQLQMKPVEGRDIIVIKMAKDFPPFPQKLKFRQPT
                     IKDRVCMVSTNFQQKSVSSLVSESSHIVHKEDTSFWQHWITTKDGQCGSPLVSIIDGNI
                     LGIHSLTHTTNGSNYFVEFPEKFVATYLDAADGWCKNWKFNADKISWGSFTLVEDAPED
                     DFMAKKTVAAIMD"
     protein_bind    complement(720..744)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(757..780)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 gaagctgggt tattagtcca tgatggcggc aacagttttt ttggccatga agtcatcttc
       61 cggcgcatct tcaaccaggg taaaggaacc ccagctgatt ttatccgcgt tgaatttcca
      121 gtttttgcac caaccatccg cggcatctag ataagtcgcc acgaattttt ccggaaattc
      181 cacgaagtag ttgctaccgt tggtggtatg agtcaggctg tggatgccca gaatgttgcc
      241 atcaatgatg gaaactagtg ggctgccaca ctggccatct ttagtggtga tccagtgctg
      301 ccagaaagaa gtgtcttctt tatgcacaat gtgagaggat tcagacacca ggctcgagac
      361 gcttttctgc tgaaagttgg tggacaccat gcacacacga tctttgatgg tcggctgacg
      421 gaatttcagt ttctgcggga acggcgggaa gtctttagcc attttgataa cgataatgtc
      481 acggccttca accggtttca tctgcagctg ggtagagttt ttgactttga attcaccatg
      541 catggttttg atggtcagtt cgccattgtt acgacgaaac agatgctggt tggcaatgat
      601 atacgggcca aaaccaatgc caaacagacg ttcactatga ccatccgagg agttttccag
      661 caggcatacg caagcagaga tcggattaaa atcgcgcacg cccttcagca aagctttcga
      721 gcctgctttt ttgtacaaac ttgtgatcga attagtctgc gcgtctttca gggcttcatc
      781 gacagtctga cgaccgctgg cggcgttgat caccgcagta cgcacggcat accagaaagc
      841 ggacatctgc gggatgttcg gcatgatttc acctttctgg ggggttttca tggtggcggc
      901 aatacgtgga tctttcgc
//
