LOCUS       sequence-173046-        5664 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for PPARgamma activity.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Degrelle SA, Shoaito H, Fournier T
  TITLE     New Transcriptional Reporters to Quantify and Monitor PPARgamma 
            Activity.
  JOURNAL   PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 
            Nov 1.
  PUBMED    29225614
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PPAR Res.";
            date: "2017"; volume: "2017:6139107. doi"; pages: " 
            10.1155/2017/6139107. Epub 2017 Nov 1"
COMMENT     Sequence Label: PPRE-H2B-eGFP
FEATURES             Location/Qualifiers
     source          1..5664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1302..1679
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      1692..1701
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1698..1700,1701..1703,1704..2417)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1698..1700,1701..1703,1704..2414)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1743..1764)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2004..2023)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2351..2372
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2540..2661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2577..2596)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2631..2650
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2668..3123)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2805..2826)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3017..3036
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3150..3254
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3256..3613
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3256..3447
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3279..3299)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3464..3599
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3648..4442
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3648..4442
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3702..3721)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4312..4331
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4630..4649)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4674..4721
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5050..5638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5539..5558
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
       61 tgtggataac cgtattaccg ccatgcatta gttattaata atgcctgcag gtcaattctg
      121 agggcagagc cgggccactt tctcaggcct ctgatttcat actgtggtgt tagttacttc
      181 tgagaggaca gcttgctgcc agagctctat tttttatgtt agaggctcct tctgcctgca
      241 gactctgctg tctgggaagg gcacagcgtt aggagggaga gggaggtgtg agtccctccg
      301 tggacccgct gctttgtact tctctatctc atttcctttt cagcaccact ctgggaaatc
      361 agtattccag ccccatttta tcctcagaaa attgaggctc tgagatgtta tctctgtgac
      421 ctgggtccta ttacgtgcca aaggcatcat ttaagcctaa gatgtcctgg ctccaaggtg
      481 tcagcatctg gaagacaggc gccctcatcc tgccatccct gctgcggctt cactgtgggc
      541 ccaggggaca tctcagcccc gagaagggtc agcggcccct cctggaccac cgactccccg
      601 cagaactcct ctgtgccctc tcctcaccag accttgttcc tcccagttgc tcccacagcc
      661 agggggcagt gagggctgct cttcccccag ccccactgag gaacccagga aggtgaacga
      721 gagaatcagt cctggtgggg gctggggagg gccccagaca tgagaccagc tcctccccca
      781 ggggatgtta tcagtgggtc cagagggcaa aatagggagc ctggtggagg gaggggcctg
      841 aaattccagt gtgaaaggct gagatgggcc cgaggcccct ggcctatgtc caagccattt
      901 cccctctcac cagcctctcc ctggggagcc agtcagctag gaaggaatga gggctcccca
      961 ggcccacccc agttcctgag ctcatctggg ctgcagggct ggcgggacag cagcgtggac
     1021 tcagtctcct agggatttcc caactctccc gcccgcttgc tgcatctgga caccctgcct
     1081 caggccctca tctccactgg tcagcaggtg acctttgccc agcgccctgg gtcctcagtg
     1141 cctgctgccc tggagatgat ataaaacagg tcagaaccct cctgcctgtc tgctcagttc
     1201 atccctagag gcaggactct agaggatctg cgatcgctag cgctaccgga ctcagatctc
     1261 gagctcaagc ttcgaattct gcagtcgacg gtaccgccac catgccagag ccagcgaagt
     1321 ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg
     1381 gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga
     1441 agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg
     1501 tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc
     1561 gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt
     1621 tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg
     1681 atccaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     1741 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     1801 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     1861 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
     1921 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     1981 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2041 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2101 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     2161 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     2221 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2281 tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga
     2341 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2401 acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt
     2461 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2521 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2581 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2641 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     2701 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2761 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     2821 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     2881 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     2941 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3001 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3061 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3121 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3181 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3241 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     3301 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3361 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3421 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3481 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3541 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3601 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3661 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3721 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3781 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     3841 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3901 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3961 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4021 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     4081 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4141 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     4201 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     4261 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     4321 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4381 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4441 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     4501 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4561 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     4621 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     4681 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     4741 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     4801 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     4861 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     4921 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4981 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5041 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5101 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5161 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5221 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5281 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5341 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5401 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5461 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5521 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5581 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5641 cgccagcaac gcggcctttt tacg
//
