LOCUS       sequence-17312-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Duffy S, Tsao KL, Waugh DS
  TITLE     Site-specific, enzymatic biotinylation of recombinant proteins in 
            Spodoptera frugiperda cells using biotin acceptor peptides.
  JOURNAL   Anal Biochem. 1998 Sep 10. 262(2):122-8.
  PUBMED    9750126
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 1998 Sep 10. 262(2):122-8."
COMMENT     Sequence Label: pDW445
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..76)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     promoter        complement(95..204)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        329..420
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     345..362
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 ttctagaggg cccgggcgcg ccaccagtat cttcatgcca ttcaattttt tgtgcttcaa
       61 agatatcatt caggcctcct gccatggtgc gtacgattgt aaataaaatg taatttacag
      121 tatagtattt taattaatat acaaatgatt tgataataat tcttatttaa ctataatata
      181 ttgtgttggg ttgaattaaa ggtcccggca tcctcaaatg cataatatca tagtccccct
      241 tgttgtaagt gatgcgtatt tctgaatctt tgtaaaatag cacacaggac tccaacgcgt
      301 ttggcgtttt attttcttgc tcgaggatat catggagata attaaaatga taaccatctc
      361 gcaaataaat aagtatttta ctgttttcgt aacagttttg taataaaaaa acctataaat
      421 attccggatt attcataccg tcccaccatc gggcgtgcta gcggatccat gaaggataac
      481 accgtgccac tgaaattgat tgccctgtta gcgaacggtg aatttcactc tggcgagcag
      541 ttgggtgaaa cgctgggaat gagccgggcg gctattaata aacacattca gacactgcgt
      601 gactggggcg ttgatgtctt taccgttccg ggtaaaggat acagcctgcc tgagcccatc
      661 cagttactta atgctgaaca gatattg
//
