LOCUS       sequence-17314-e         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Duffy S, Tsao KL, Waugh DS
  TITLE     Site-specific, enzymatic biotinylation of recombinant proteins in 
            Spodoptera frugiperda cells using biotin acceptor peptides.
  JOURNAL   Anal Biochem. 1998 Sep 10. 262(2):122-8.
  PUBMED    9750126
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 1998 Sep 10. 262(2):122-8."
COMMENT     Sequence Label: pDW464
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(26..70)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     promoter        complement(89..198)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     promoter        323..414
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     339..356
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
ORIGIN
        1 agggcccggg cgcgccacca gtatcttcat gccattcaat tttttgtgct tcaaagatat
       61 cattcaggcc tcctgccatg gtgcgtacga ttgtaaataa aatgtaattt acagtatagt
      121 attttaatta atatacaaat gatttgataa taattcttat ttaactataa tatattgtgt
      181 tgggttgaat taaaggtccc ggcatcctca aatgcataat atcatagtcc cccttgttgt
      241 aagtgatgcg tatttctgaa tctttgtaaa atagcacaca ggactccaac gcgtttggcg
      301 ttttattttc ttgctcgagg atatcatgga gataattaaa atgataacca tctcgcaaat
      361 aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat aaatattccg
      421 gattattcat accgtcccac catcgggcgt gctagcggat ccatgaagga taacaccgtg
      481 ccactgaaat tgattgccct gttagcgaac ggtgaatttc actctggcga gcagttgggt
      541 gaaacgctgg gaatgagccg ggcggctatt aataaacaca ttcagacact gcgtgactgg
      601 ggcgttgatg tctttaccgt tccgggtaaa ggatacagcc tgcctgagcc catccagtta
      661 cttaatgctg aacagatatt gggtcagctg gatggcggta gtgtagccgt gctgccagtt
      721 attgactcca cgaatcagta ccttcttgat cgtatcggag agcttaaatc gggcgatgcc
      781 tgtgttgcag aataccaaca ggctggccgt ggtcgccggg ggcggaaatg gttttcgcct
      841 tttgg
//
