LOCUS       sequence-173160-        7353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7353)
  AUTHORS   McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
  TITLE     A bright and photostable photoconvertible fluorescent protein.
  JOURNAL   Nat Methods. 2009 Feb . 6(2):131-3.
  PUBMED    19169260
REFERENCE   2  (bases 1 to 7353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2009 Feb . 6(2):131-3."
COMMENT     Sequence Label: mEos2-Desmocollin1-N-18
FEATURES             Location/Qualifiers
     source          1..7353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2737..3417
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3539..3660
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3576..3595)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3630..3649
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3667..4122)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3804..3825)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4016..4035
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4149..4253
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4255..4612
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4255..4446
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4278..4298)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4463..4598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4647..5441
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4647..5441
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4701..4720)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5311..5330
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5629..5648)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5673..5720
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6049..6637
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6538..6557
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        6812..7115
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7116..7319
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7270..7290
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      join(7348..7353,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggctctgg cctctgctgc cccagggagc atcttctgta agcagctcct tttctctctc
       61 ctggttttaa cattactttg cgatgcttgt cagaaagttt atcttcgagt tccttctcat
      121 cttcaggctg aaacacttgt aggcaaagtg aatctggagg agtgtctcaa gtcggccagc
      181 ctaatccggt ccagtgaccc tgccttcagg attctagaag atggctcaat ttacacaaca
      241 catgacctca ttttgtcttc tgaaaggaaa agtttttcca ttttcctttc agatggtcag
      301 agacgggaac aacaagagat aaaagttgta ctgtcagcaa gagaaaacaa gtctcctaag
      361 aagagacata ccaaagacac agccctcaag cgcagcaaga gacgatgggc tcctattcca
      421 gcttcattga tggagaactc gttgggtcca tttccacaac acgttcagca gatccaatct
      481 gatgctgcac agaattacac catcttttat tccataagtg ggccaggcgt ggacaaagaa
      541 cccttcaatt tgttttacat agagaaagac actggggata tcttttgtac aaggagcatt
      601 gaccgtgaga aatacgaaca gtttgcgtta tatggctatg caacaactgc agatggctat
      661 gcaccagaat atccactccc tttgatcatc aaaattgaag atgataatga taacgcccca
      721 tattttgaac acagagtgac tatctttact gtgcctgaaa attgccgatc cggcacttca
      781 gtgggaaaag tgaccgccac agaccttgac gaacctgaca ctctccatac tcgtctgaaa
      841 tataaaatct tacaacaaat cccagatcat ccaaagcatt tctccataca cccagatacc
      901 ggagtcatca ccacaactac accttttctg gatagagaaa aatgtgatac ttaccagtta
      961 ataatggaag tgcgagacat gggtggtcag cctttcggtt tatttaatac aggaacaatt
     1021 actatttcac ttgaggatga aaatgacaat ccaccatctt tcacagaaac ttcttatgtt
     1081 acagaagtag aagaaaacag aattgacgtg gagattttgc gaatgaaggt acaggatcag
     1141 gatttgccaa acactcctca ctcaaaggct gtatacaaaa tcttacaagg aaatgaaaat
     1201 ggaaacttca taattagcac agatccaaat acaaatgaag gagtgctgtg tgttgtcaag
     1261 ccattgaact atgaagtcaa tcgccaagtt attttgcaag ttggtgtcat taacgaggca
     1321 caattctcta aagcagcgag ctcacaaact cctacaatgt gcactacaac tgtcaccgtt
     1381 aaaattatag acagtgatga gggccctgaa tgccaccctc cagtgaaagt tattcagagt
     1441 caagatggct tcccagctgg ccaagaactc cttggataca aagcactgga cccggaaata
     1501 tccagtggtg aaggcttaag gtatcagaag ttaggggatg aagataactg gtttgaaatt
     1561 aatcaacaca ctggcgactt gagaactcta aaagtactag atagagaatc caaatttgta
     1621 aaaaacaacc aatacaatat ttcagttgtt gcagtggatg cagttggccg atcttgcact
     1681 ggaacattag tagttcattt ggatgattac aacgatcacg cacctcaaat tgacaaagaa
     1741 gtgaccattt gtcagaataa tgaggatttt gctgttctga aacctgtaga tccagatgga
     1801 cctgaaaatg gaccaccttt tcaattcttt ctggataatt ctgccagtaa aaactggaac
     1861 atagaagaaa aggatggtaa aactgccatt cttcgtcaac ggcaaaatct tgattataac
     1921 tattattctg tgcctattca aataaaagac aggcatggtt tagttgcaac acatatgtta
     1981 acagtgagag tatgtgactg ttcaactcca tctgagtgta gaatgaagga taaaagtaca
     2041 agagacgtta gaccaaatgt aatacttgga agatgggcta ttcttgctat ggtgttgggt
     2101 tctgtattgt tattatgtat tctgtttaca tgtttctgtg tcactgctaa gagaacagtc
     2161 aagaaatgtt ttccagaaga catagcccag caaaatttaa ttgtatcaaa tactgaagga
     2221 cctggagaag aagtaacgga agcaaatatt agactcccca tgcagacatc caacatttgt
     2281 gacacaagca tgtctgttgg tactgttggt ggccagggaa tcaaaacaca gcaaagtttt
     2341 gagatggtca aaggaggcta cactttggat tccaacaaag gaggtggaca tcagaccttg
     2401 gagtccgtca agggagtggg gcagggagat actggcagat atgcgtacac ggactggcag
     2461 agtttcaccc aacctcggct tggcgaaaag gtgtatttgt gtggacaaga tgaggagcat
     2521 aaacattgtg aagactacgt ttgttcgtat aactatgaag gcaaaggttc tctggccggc
     2581 tcagtaggtt gctgcagcga tcggcaggaa gaagagggac tggagtttct agatcacctg
     2641 gaacccaaat ttaggacatt agcaaagaca tgcatcaaga aaagcagcgg tggagcaagc
     2701 gcagccagtg gtagcgcgga tccaccggtc gccaccatga gtgcgattaa gccagacatg
     2761 aagatcaaac tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
     2821 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
     2881 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
     2941 gccaaatatc cagacaacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
     3001 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
     3061 atggaagggg acactttcta taataaagtt cgattttatg gtaccaactt tcccgccaat
     3121 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
     3181 cgtgatggag tgctgacggg tgatattcat atggctttgt tgcttgaagg aaatgcccat
     3241 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     3301 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
     3361 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgataagcg
     3421 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3481 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3541 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3601 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3661 aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     3721 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     3781 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     3841 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     3901 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     3961 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     4021 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     4081 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     4141 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4201 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
     4261 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4321 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4381 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4441 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4501 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4561 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc
     4621 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     4681 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4741 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4801 acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca
     4861 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     4921 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     4981 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5041 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5101 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     5161 ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     5221 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     5281 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     5341 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     5401 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga
     5461 aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt
     5521 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     5581 cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa
     5641 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
     5701 cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
     5761 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
     5821 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
     5881 catagcctca ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5941 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6001 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6061 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6121 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6181 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6241 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6301 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6361 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6421 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6481 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6541 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6601 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6661 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     6721 ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt
     6781 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     6841 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     6901 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     6961 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     7021 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     7081 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     7141 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     7201 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     7261 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
     7321 gtttagtgaa ccgtcagatc cgctagcgcc acc
//
