LOCUS       sequence-173161-        5310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5310)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-Rab4a-7
FEATURES             Location/Qualifiers
     source          1..5310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..678
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    1486..1607
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1523..1542)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1577..1596
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1614..2069)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1751..1772)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1963..1982
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2096..2200
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2202..2559
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2202..2393
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2225..2245)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2410..2545
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2594..3388
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2594..3388
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2648..2667)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3258..3277
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3576..3595)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3620..3667
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3996..4584
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4485..4504
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4759..5062
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5063..5266
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5217..5237
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgagtgcga ttaagccaga catgaagatc aaactccgta tggaaggcaa cgtaaacggg
       61 caccactttg tgatcgacgg agatggtaca ggcaagcctt ttgagggaaa acagagtatg
      121 gatcttgaag tcaaagaggg cggacctctg ccttttgcct ttgatatcct gaccactgca
      181 ttccattacg gcaacagggt attcgccaaa tatccagaca acatacaaga ctattttaag
      241 cagtcgtttc ctaaggggta ttcgtgggaa cgaagcttga ctttcgaaga cgggggcatt
      301 tgcattgcca gaaacgacat aacaatggaa ggggacactt tctataataa agttcgattt
      361 tatggtacca actttcccgc caatggtcca gttatgcaga agaagacgct gaaatgggag
      421 ccctccactg agaaaatgta tgtgcgtgat ggagtgctga cgggtgatat tcatatggct
      481 ttgttgcttg aaggaaatgc ccattaccga tgtgacttca gaactactta caaagctaag
      541 gagaagggtg tcaagttacc aggctaccac tttgtggacc actgcattga gattttaagc
      601 catgacaaag attacaacaa ggttaagctg tatgagcatg ctgttgctca ttctggattg
      661 cctgacaatg ccagacgatc cggactcaga tctcgagcca tgtcgcagac ggccatgtcc
      721 gaaacctacg attttttgtt taagttcttg gttattggaa atgcaggaac tggcaaatct
      781 tgcttacttc atcagtttat tgaaaaaaaa ttcaaagatg actcaaatca tacaatagga
      841 gtggaatttg gttcaaagat aataaatgtt ggtggtaaat atgtaaagtt acaaatatgg
      901 gatacagcag gacaagaacg attcaggtcc gtgacgagaa gttattaccg aggcgcggcc
      961 ggggctctcc tcgtctatga tatcaccagc cgagaaacct acaatgcgct tactaattgg
     1021 ttaacagatg cccgaatgct ggcgagccag aacattgtga tcatcctttg tggaaacaag
     1081 aaggacctgg atgcagatcg tgaagttacc ttcttagaag cctccagatt tgctcaagaa
     1141 aatgagctga tgtttttgga aacaagtgcg ctcacagggg agaatgtaga agaggctttt
     1201 gtacagtgtg caagaaaaat acttaacaaa atcgaatcag gtgagctgga cccagaaaga
     1261 atgggctcag gtattcagta cggagatgct gccttgagac agctgaggtc accgcggcgc
     1321 gcacaggccc cgaacgctca ggagtgtggt tgttagggat ccaccggatc tagataactg
     1381 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1441 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1501 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1561 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt
     1621 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     1681 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     1741 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     1801 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     1861 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     1921 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     1981 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     2041 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     2101 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2161 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     2221 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     2281 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     2341 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     2401 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2461 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     2521 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     2581 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     2641 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     2701 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     2761 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     2821 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     2881 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     2941 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3001 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     3061 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     3121 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     3181 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     3241 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     3301 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     3361 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     3421 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     3481 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     3541 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     3601 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     3661 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     3721 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     3781 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     3841 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     3901 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     3961 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4021 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4081 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4141 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4201 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4261 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4321 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4381 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4441 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4501 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4561 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4621 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4681 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag
     4741 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     4801 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     4861 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     4921 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     4981 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     5041 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     5101 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     5161 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     5221 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg
     5281 tcagatccgc tagcgctacc ggtcgccacc
//
