LOCUS       sequence-173164-        5330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  araBAD based expression vector for inducible protein production in 
            bacterial hosts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Sommer M, Xie H, Michel H
  TITLE     Pseudomonas stutzeri as an alternative host for membrane proteins.
  JOURNAL   Microb Cell Fact. 2017 Sep 20;16(1):157. doi: 
            10.1186/s12934-017-0771-0.
  PUBMED    28931397
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-09-20"; volume: "16(1):157. doi"; pages: " 
            10.1186/s12934-017-0771-0"
COMMENT     Sequence Label: pL2020
FEATURES             Location/Qualifiers
     source          1..5330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             322..351
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     regulatory      348..357
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             403..423
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             436..465
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(505..522)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      675..721
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1340..1359
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1426..1528)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1645..1663)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2921..3692
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3693..4355
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     CDS             complement(4426..5304)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattacatat gcaccatcac catcaccatc accatcacca tggtggccgt
      361 ggttccgaaa acctgtactt tcaagatctg ccggcgaatc tagaaaacct gtactttcaa
      421 ggtggccgtg gttcccacca tcaccatcac catcaccatc accattaagc ttggctgttt
      481 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
      541 gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa
      601 ctcagaagtg aaacgccgta gcgccgatgg tagtgtgggg tctccccatg cgagagtagg
      661 gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      721 tccgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      781 gcgcatgcat aaaaactgtt gtaattcatt aagcattctg ccgacatgga agccatcaca
      841 aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt gcgtataata
      901 tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa
      961 actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa taaacccttt
     1021 agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa
     1081 ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg
     1141 gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc
     1201 catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata
     1261 aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctgaacggt
     1321 ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca
     1381 ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc
     1441 tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg
     1501 aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg
     1561 cttcccggta tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac
     1621 aggtatttat tcgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1681 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcgcccgcg
     1741 ttcctgctgg cgctgggcct gtttctggcg ctggacttcc cgctgttccg tcagcagctt
     1801 ttcgcccacg gccttgatga tcgcggcggc cttggcctgc atatcccgat tcaacggccc
     1861 cagggcgtcc agaacgggct tcaggcgctc ccgaaggtct cgggccgtct cttgggcttg
     1921 atcggccttc ttgcgcatct cacgcgctcc tgcggcggcc tgtagggcag gctcataccc
     1981 ctgccgaacc gcttttgtca gccggtcggc cacggcttcc ggcgtctcaa cgcgctttga
     2041 gattcccagc ttttcggcca atccctgcgg tgcataggcg cgtggctcga ccgcttgcgg
     2101 gctgatggtg acgtggccca ctggtggccg ctccagggcc tcgtagaacg cctgaatgcg
     2161 cgtgtgacgt gccttgctgc cctcgatgcc ccgttgcagc cctagatcgg ccacagcggc
     2221 cgcaaacgtg gtctggtcgc gggtcatctg cgctttgttg ccgatgaact ccttggccga
     2281 cagcctgccg tcctgcgtca gcggcaccac gaacgcggtc atgtgcgggc tggtttcgtc
     2341 acggtggatg ctggccgtca cgatgcgatc cgccccgtac ttgtccgcca gccacttgtg
     2401 cgccttctcg aagaacgccg cctgctgttc ttggctggcc gacttccacc attccgggct
     2461 ggccgtcatg acgtactcga ccgccaacac agcgtccttg cgccgcttct ctggcagcaa
     2521 ctcgcgcagt cggcccatcg cttcatcggt gctgctggcc gcccagtgct cgttctctgg
     2581 cgtcctgctg gcgtcagcgt tgggcgtctc gcgctcgcgg taggcgtgct tgagactggc
     2641 cgccacgttg cccattttcg ccagcttctt gcatcgcatg atcgcgtatg ccgccatgcc
     2701 tgcccctccc ttttggtgtc caaccggctc gacgggggca gcgcaaggcg gtgcctccgg
     2761 cgggccactc aatgcttgag tatactcact agactttgct tcgcaaagtc gtgaccgcct
     2821 acggcggctg cggcgcccta cgggcttgct ctccgggctt cgccctgcgc ggtcgctgcg
     2881 ctcccttgcc agcccgtgga tatgtggacg atggccgcga gcggccaccg gctggctcgc
     2941 ttcgctcggc ccgtggacaa ccctgctgga caagctgatg gacaggctgc gcctgcccac
     3001 gagcttgacc acagggattg cccaccggct acccagcctt cgaccacata cccaccggct
     3061 ccaactgcgc ggcctgcggc cttgccccat caattttttt aattttctct ggggaaaagc
     3121 ctccggcctg cggcctgcgc gcttcgcttg ccggttggac accaagtgga aggcgggtca
     3181 aggctcgcgc agcgaccgcg cagcggcttg gccttgacgc gcctggaacg acccaagcct
     3241 atgcgagtgg gggcagtcga agggcgaagc ccgcccgcct gccccccgag cctcacggcg
     3301 gcgagtgcgg gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt
     3361 cgatcaacaa gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg
     3421 ggggaacccc gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg
     3481 cgaaagactt aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac
     3541 cccccgcaat agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg
     3601 caacgcataa ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa
     3661 ccgtgcggca cccctaccgc atggagataa gcatggccac gcagtccaga gaaatcggca
     3721 ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga acgcaaagcg catgaggcgt
     3781 gggccgggct tattgcgagg aaacccacgg cggcaatgct gctgcatcac ctcgtggcgc
     3841 agatgggcca ccagaacgcc gtggtggtca gccagaagac actttccaag ctcatcggac
     3901 gttctttgcg gacggtccaa tacgcagtca aggacttggt ggccgagcgc tggatctccg
     3961 tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt ggtcaatgac cgcgtggcgt
     4021 ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag tgccgccgtg gtggttgatc
     4081 acgacgacca ggacgaatcg ctgttggggc atggcgacct gcgccgcatc ccgaccctgt
     4141 atccgggcga gcagcaacta ccgaccggcc ccggcgagga gccgcccagc cagcccggca
     4201 ttccgggcat ggaaccagac ctgccagcct tgaccgaaac ggaggaatgg gaacggcgcg
     4261 ggcagcagcg cctgccgatg cccgatgagc cgtgttttct ggacgatggc gagccgttgg
     4321 agccgccgac acgggtcacg ctgccgcgcc ggtagcactt gggttgcgca gcaacccgta
     4381 agtgcgctgt tccagactat cggctgtagc cgcctcgccg cccaattatg acaacttgac
     4441 ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc tggccccggt
     4501 gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac
     4561 ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc
     4621 ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga
     4681 cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg
     4741 atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc
     4801 gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc
     4861 cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg
     4921 cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg acggccagtt
     4981 aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg
     5041 agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc
     5101 cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag
     5161 attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt
     5221 ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag
     5281 gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
//
