LOCUS       sequence-173165-        8145 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Chromatin Assembly Factor 1, Excitation: 554, 
            Emission: 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8145)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 8145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTomato-CAF1-C-10
FEATURES             Location/Qualifiers
     source          1..8145
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..1428
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     protein_bind    3247..3258
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     polyA_signal    4321..4442
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4358..4377)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4412..4431
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4449..4904)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4586..4607)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4798..4817
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4931..5035
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5037..5394
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5037..5228
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5060..5080)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5245..5380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5429..6223
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5429..6223
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5483..5502)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6093..6112
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6411..6430)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6455..6502
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6831..7419
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7320..7339
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7594..7897
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7898..8101
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8052..8072
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      join(8140..8145,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga ggtcatcaaa gagttcatgc gcttcaaggt gcgcatggag
       61 ggctccatga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      121 ggcacccaga ccgccaagct gaaggtgacc aagggcggcc ccctgccctt cgcctgggac
      181 atcctgtccc cccagttcat gtacggctcc aaggcgtacg tgaagcaccc cgccgacatc
      241 cccgattaca agaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      301 gaggacggcg gtctggtgac cgtgacccag gactcctccc tgcaggacgg cacgctgatc
      361 tacaaggtga agatgcgcgg caccaacttc ccccccgacg gccccgtaat gcagaagaag
      421 accatgggct gggaggcctc caccgagcgc ctgtaccccc gcgacggcgt gctgaagggc
      481 gagatccacc aggccctgaa gctgaaggac ggcggccact acctggtgga gttcaagacc
      541 atctacatgg ccaagaagcc cgtgcaactg cccggctact actacgtgga caccaagctg
      601 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgagcg ctccgagggc
      661 cgccaccacc tgttcctggg gcatggcacc ggcagcaccg gcagcggcag ctccggcacc
      721 gcctcctccg aggacaacaa catggccgtc atcaaagagt tcatgcgctt caaggtgcgc
      781 atggagggct ccatgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      841 tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct gcccttcgcc
      901 tgggacatcc tgtcccccca gttcatgtac ggctccaagg cgtacgtgaa gcaccccgcc
      961 gacatccccg attacaagaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     1021 aacttcgagg acggcggtct ggtgaccgtg acccaggact cctccctgca ggacggcacg
     1081 ctgatctaca aggtgaagat gcgcggcacc aacttccccc ccgacggccc cgtaatgcag
     1141 aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga cggcgtgctg
     1201 aagggcgaga tccaccaggc cctgaagctg aaggacggcg gccactacct ggtggagttc
     1261 aagaccatct acatggccaa gaagcccgtg caactgcccg gctactacta cgtggacacc
     1321 aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgagcgctcc
     1381 gagggccgcc accacctgtt cctgtacggc atggacgagc tgtacaagtc cggactcaga
     1441 tctggcagcg gtggaggctt ggaggagccg gaggccgcga cgcggacagc cgcggccgtg
     1501 gattgcaaag acagacccgg gttcccagtg aagaggttaa tacaagcccg cttgcccttc
     1561 aaacgcctga accttgttcc caaggagaaa gttgaggaag acacatcacc aaaagcagct
     1621 gtggaaagca aagtcccgga tctacaattg tctttgggca cttttgaaag ccagtgtcac
     1681 acaggctctc acgtaggctt gagtacaaag ttggtcggtg gccagggccc cattgatagc
     1741 ttcttgaggg caacaatcaa gccagtaccg agtgtggtca ttatcgacct gacggagaac
     1801 tgcagtgaca ttccagacag ccctgagggc cacagtgaac taagtcctga cactgctggt
     1861 gtggtcacca cagtggaagg agctgccaag cagcaggagc actctgcagc agagctgtgt
     1921 ctcctagaga ccccttcaga catcacttgt cacatggaag aggagcctgg gagtccagga
     1981 gacccaaaga ggacgggtga ctgtcaggct ggttcactac agagctgtcc ggagctgacc
     2041 ccaggttcta ggacatgccc caccaaggag ttatcaagct ggagcaaggc aggggatctc
     2101 ctatttatag agaaagtgcc tgtggtggtg ctagaggaca tcctggccac taagccctcc
     2161 attgcctctc ttcccatgat gtccctggat aggagtgtca cttcagagag tgaaatactg
     2221 gagtcctgcc ccgaggatga ctccatactg agccactcct ccaccaactc ctcttctcca
     2281 accagctctc ctgagggacc atccacaccc ccagaacatc ggggtgggag aagctccccc
     2341 tccaccccag cctgcagagt tgccaagaac tttgtcaaag gctccacaga gaagggcagg
     2401 agcaaactgc acagagatag agaacagcag agggaagaga aggaaaagct gagggaagag
     2461 atacgacgag ccaaggagga ggcacggaag aagaaggagg aggagaagga gctgaaggaa
     2521 aaggagcgga gggaaaagcg ggagaaagat gagaaggaga aggcggagaa gcagcggcta
     2581 aaggaggaaa aacgcaagga gcggcaggaa gctctggagg ctaaactgga ggagaagagg
     2641 aagaaggagg aagagaagcg gttacgggag gaagagaagc ggttacggga agaagagaag
     2701 cgcattaagg cagaaaaggc agagatcact cgattcttcc agaaaccgaa gactccgcag
     2761 gctcccaaga ccctggccgg ttcctgtggt aagtttgccc cctttgaaat caaagaacac
     2821 atggtgctgg ctccacggtg ccgagctgcc ttggatcaag acctgtgcga tcagctggac
     2881 cagctcctgc agcagcagag tgtggcaagc accttcctca gtgacctaaa aagccggctg
     2941 cccttgagat cggggcccac ccgggtctgt ggccatgaca cagacatcat gaacagggat
     3001 gtggtcattg tggagagcag caaggtggac ggtgtgtctg aaaggaagaa gtttggccgc
     3061 atgaagcttc tgcagttctc agagaaccac cggccagcat actgggggac gtggaacaag
     3121 aagacagcca tcatccgccc ccggaacccc tgggcccagg acaaagacct tcttgactat
     3181 gaggtagaca gtgacgatga gtgggaagag gaggaacctg gggagtcttt gtcccacagt
     3241 gagggggatg aggatgacga tgtgggggaa gatgaggatg aggacgatgg cttctttgtg
     3301 ccccatgggt acttatctga agatgaaggc gtgactgagg agtgtgcaga ccctgagaac
     3361 cacaaagtac accagaagct gaaggccaag gagtgggatg agctcctagc caagggcaag
     3421 cgctttcgtg tgctgcagcc tgtgcacgtg ggatgtgtat gggcatcaga ggcagccaac
     3481 tgcacgagtt ccgacttgaa gctgctgcag cagttcactg catgcctgct ggatgtggca
     3541 tcccctgatg agccagaacc cggggcctcc aggagggaaa agagggacca gcatatcctg
     3601 gcccagttgc ttccgctgct acatggcaat gtgaatggga gcaaggtgat catccatgaa
     3661 ttccaggagc aatgccgcag gggcctgctg acccttccaa gtcccacccc acacctgcag
     3721 atgcccaact tggaggatgc tgttgctgtg ccatccaagg ccaggctgaa gcgccttatc
     3781 tctgagaact ctgcctatga gaagcggccc aacttccgta tgtgctggta tgtgcaccca
     3841 gaggtgctca agagctttgg ccaggagtgc ctgccagtgc cctgccagtg gacctacatc
     3901 accaccatgc cctcagcacc cagggaagac agtggcagtg cttctactga ggggcctggc
     3961 cagagcacac ctatgctact caagcggaag ccagcggcca ccatgtgcat cacgcagttt
     4021 atgaagaagc ggcgctatga tggacaggtt gggtctgggg acatggatgg cttccaggca
     4081 gacacagaag aggatgaaga ggatgacaca gactgtatga tcatagatgt gccagatgtt
     4141 gggagtgacg tgtcagaagc tcccatacct gctcccacac tttgcaagtg aggatccacc
     4201 ggatctagat aactgatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4261 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4321 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4381 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4441 taacgcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4501 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4561 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4621 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     4681 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     4741 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     4801 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     4861 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
     4921 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     4981 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
     5041 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     5101 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     5161 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     5221 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     5281 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     5341 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
     5401 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     5461 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     5521 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     5581 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
     5641 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     5701 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     5761 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     5821 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     5881 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     5941 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6001 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6061 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6121 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6181 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6241 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     6301 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6361 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
     6421 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
     6481 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
     6541 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
     6601 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
     6661 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
     6721 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6781 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6841 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6901 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6961 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     7021 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7081 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7141 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7201 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7261 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7321 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7381 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7441 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     7501 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
     7561 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     7621 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     7681 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     7741 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     7801 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     7861 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     7921 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     7981 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     8041 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     8101 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccacc
//
