LOCUS       sequence-173166-        6275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 690, Emission: 713.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6275)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 6275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: iRFP-Cx43-7
FEATURES             Location/Qualifiers
     source          1..6275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1162..1171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1168..2118
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     polyA_signal    2240..2361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2277..2296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2331..2350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2368..2823)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2505..2526)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2717..2736
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2850..2954
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2956..3313
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2956..3147
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2979..2999)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3164..3299
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3348..4142
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3348..4142
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3402..3421)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4012..4031
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4330..4349)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4374..4421
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4750..5338
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5239..5258
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5513..5816
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5817..6020
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5971..5991
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgggtgact ggagtgcctt ggggaagctt ctggacaagg tccaagccta ctccaccgct
       61 ggagggaagg tgtggctgtc agtgctcttc atattcagaa tcctgctcct ggggacagct
      121 gttgagtcag cttggggtga tgaacagtct gcctttcgct gtaacactca acaacctggc
      181 tgcgaaaacg tctgctatga caagtccttc cccatctctc acgtgcgctt ctgggtcctt
      241 cagatcatat tcgtgtctgt gcccacactc ctgtacttgg cccatgtgtt ctatgtgatg
      301 aggaaggaag agaagctaaa caagaaagaa gaggagctca aagtggccca gactgacggg
      361 gtcaacgtgg agatgcacct gaagcagatt gaaatcaaga agttcaagta cgggattgaa
      421 gagcacggca aggtgaaaat gaggggcggc ttgctgagaa cctacatcat cagcatcctc
      481 ttcaagtctg tcttcgaggt ggccttcctg ctcatccagt ggtacatcta tgggttcagc
      541 ttgagcgcgg tctacacctg caagagagat ccctgccccc accaggtaga ctgcttcctc
      601 tcacgtccca cggagaaaac catcttcatc atcttcatgc tggtggtgtc cttggtgtct
      661 ctcgctttga acatcattga gctcttctac gtcttcttca agggcgttaa ggatcgcgtg
      721 aagggaagaa gcgatcctta ccacgccacc actggcccac tgagcccatc aaaagactgc
      781 ggatctccaa aatacgccta cttcaatggc tgctcctcac caacggctcc actctcgcct
      841 atgtctcctc ctgggtacaa gctggttact ggtgacagaa acaattcctc gtgccgcaat
      901 tacaacaagc aagctagcga gcaaaactgg gcgaactaca gcgcagagca aaatcgcatg
      961 gggcaggccg gaagcaccat ctccaactcg cacgcccagc cgttcgattt ccccgacgac
     1021 aaccagaatg ccaaaaaagt tgctgctgga catgaactcc agccattagc catcgtggac
     1081 caacgacctt ccagcagagc cagcagccgc gccagcagca ggcctcggcc tgatgacctg
     1141 gagattgcgg atccaccggt cgccaccatg gcggaaggat ctgtcgccag gcagcctgac
     1201 ctcttgacct gcgacgatga gccgatccat atccccggtg ccatccaacc gcatggactg
     1261 ctgctcgccc tcgccgccga catgacgatc gttgccggca gcgacaacct tcccgaactc
     1321 accggactgg cgatcggcgc cctgatcggc cgctctgcgg ccgatgtctt cgactcggag
     1381 acgcacaacc gtctgacgat cgccttggcc gagcctggcg cggccgtcgg agcaccgatc
     1441 actgtcggct tcacgatgcg aaaggacgca ggcttcatcg gctcctggca tcgccatgat
     1501 cagctcatct tcctggaatt ggagcctccc cagcgggacg tcgccgagcc gcaggcgttc
     1561 ttccgccgca ccaacagcgc catccgccgc ctgcaggccg ccgaaacctt ggaaagcgcc
     1621 tgcgccgccg cggcgcaaga ggtgcggaag attaccggct tcgatcgggt gatgatctat
     1681 cgcttcgcct ccgacttcag cggcgaagtg atcgcagagg atcggtgcgc cgaggtcgag
     1741 tcaaaactag gcctgcacta tcctgcctca accgtgccgg cgcaggcccg tcggctctat
     1801 accatcaacc cggtacggat cattcccgat atcaattatc ggccggtgcc ggtcacccca
     1861 gacctcaatc cggtcaccgg gcggccgatt gatcttagct tcgccatcct gcgcagcgtc
     1921 tcgcccgtcc atctggaatt catgcgcaac ataggcatgc acggcacgat gtcgatctcg
     1981 attttgcgcg gcgagcgact gtggggattg atcgtttgcc atcaccgaac gccgtactac
     2041 gtcgatctcg atggccgcca agcctgcgag ctagtcgccc aggttctggc ctggcagatc
     2101 ggcgtgatgg aagagtgagc ggccgcgact ctagatcata atcagccata ccacatttgt
     2161 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2221 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2341 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     2401 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2461 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     2521 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     2581 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     2641 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     2701 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     2761 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     2821 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     2881 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     2941 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     3001 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3061 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3121 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3181 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3241 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3301 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3361 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3421 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3481 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     3541 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     3601 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     3661 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     3721 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     3781 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     3841 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     3901 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     3961 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     4021 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4081 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4141 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     4201 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4261 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     4321 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     4381 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     4441 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     4501 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     4561 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     4621 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     4681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4861 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5401 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt
     5461 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     5521 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     5581 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     5641 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     5701 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     5761 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     5821 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     5881 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     5941 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     6001 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc taccggactc
     6061 agatctcgag ctcaagcttc gaattcggca gcacttttct ttcattgggg ggaaggcgtg
     6121 aggaaagtac caaacagcag cagactttta aactttaaac agacaggtct gagagcctga
     6181 actctcattt ttcctttgac ttcagcctcc aaggagttcc accaactttg gcgcgccggc
     6241 ttcactttca ttaagtgaaa gagaggtgcc cagac
//
