LOCUS       sequence-173167-       10435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of MevTaco-PMKco-MKco(aka 2B) under lacUV5 promoter 
            for isopentenol production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   George KW, Thompson MG, Kang A, Baidoo E, Wang G, Chan LJ, Adams PD,
            Petzold CJ, Keasling JD, Lee TS
  TITLE     Metabolic engineering for the high-yield production of 
            isoprenoid-based C(5) alcohols in E. coli.
  JOURNAL   Sci Rep. 2015 Jun 8;5:11128. doi: 10.1038/srep11128.
  PUBMED    26052683
REFERENCE   2  (bases 1 to 10435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-8"; volume: "5:11128. doi"; pages: " 
            10.1038/srep11128"
COMMENT     Sequence Label: JBEI-6823
FEATURES             Location/Qualifiers
     source          1..10435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(49..1131)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     1093..1112
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1132..1209)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1415..1434
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(1520..1525,1526..1543,1544..1550)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    1558..1574
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(2309..2320)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5219..5230
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      8600..8671
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8687..8714
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(8742..8759)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8876..9421)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(8956..8975)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(9535..9629)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(9650..10309)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     10224..10243
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(10310..10412)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 gacgtcggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct
       61 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      121 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag
      181 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg
      241 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc
      301 ttcggtatcg tcgtatccca ctaccgagat gtccgcacca acgcgcagcc cggactcggt
      361 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac
      421 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc
      481 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag
      541 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc
      601 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact
      661 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc
      721 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg
      781 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat
      841 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg
      901 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc
      961 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac
     1021 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg
     1081 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac
     1141 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc
     1201 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca
     1261 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg
     1321 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca
     1381 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag
     1441 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga
     1501 tcgtttaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggaattg
     1561 tgagcggata acaatttcag aattcaaaag atcttaggag gaatataaaa tgaaaaattg
     1621 tgtcatcgtc agtgcggtac gtactgctat cggtagtttt aacggttcac tcgcttccac
     1681 cagcgccatc gacctggggg cgacagtaat taaagccgcc attgaacgtg caaaaatcga
     1741 ttcacaacac gttgatgaag tgattatggg taacgtgtta caagccgggc tggggcaaaa
     1801 tccggcgcgt caggcactgt taaaaagcgg gctggcagaa acggtgtgcg gattcacggt
     1861 caataaagta tgtggttcgg gtcttaaaag tgtggcgctt gccgcccagg ccattcaggc
     1921 aggtcaggcg cagagcattg tggcgggggg tatggaaaat atgagtttag ccccctactt
     1981 actcgatgca aaagcacgct ctggttatcg tcttggagac ggacaggttt atgacgtaat
     2041 cctgcgcgat ggcctgatgt gcgccaccca tggttatcat atggggatta ccgccgaaaa
     2101 cgtggctaaa gagtacggaa ttacccgtga aatgcaggat gaactggcgc tacattcaca
     2161 gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca gccgaaatcg tcccggtaaa
     2221 tgttgtcact cgaaagaaaa ccttcgtctt cagtcaagac gagttcccga aagcgaactc
     2281 aacggctgaa gcgttaggtg cattgcgccc ggccttcgat aaagcaggaa cagtcaccgc
     2341 tgggaacgcg tctggtatta acgacggtgc tgccgctctg gtgattatgg aagaatctgc
     2401 ggcgctggca gcaggcctta cccccctggc tcgcattaaa agttatgcca gcggtggcgt
     2461 gccccccgca ttgatgggta tggggccagt acctgccacg caaaaagcgt tacaactggc
     2521 ggggctgcaa ctggcggata ttgatctcat tgaggctaat gaagcatttg ctgcacagtt
     2581 ccttgccgtt gggaaaaacc tgggctttga ttctgagaaa gtgaatgtca acggcggggc
     2641 catcgcgctc gggcatccta tcggtgccag tggtgctcgt attctggtca cactattaca
     2701 tgccatgcag gcacgcgata aaacgctggg gctggcaaca ctgtgcattg gcggcggtca
     2761 gggaattgcg atggtgattg aacggttgaa ttgaggatct aggaggaaag caaaatgaaa
     2821 ctgagcacca agctgtgctg gtgtggcatc aagggtcgcc tgcgcccaca aaagcagcaa
     2881 cagctgcaca acacgaacct gcaaatgacc gagctgaaaa agcagaagac ggccgagcaa
     2941 aagacccgcc cgcagaacgt tggcatcaag ggcatccaga tttatatccc gacgcagtgt
     3001 gtcaaccaat ctgagctgga gaaattcgat ggcgtcagcc agggtaagta caccatcggc
     3061 ctgggccaga ccaacatgag cttcgtgaac gaccgtgagg acatctattc tatgagcctg
     3121 acggtgctgt ctaagctgat caagagctac aacatcgaca cgaataagat cggtcgtctg
     3181 gaggtgggta cggagacgct gattgacaag agcaaaagcg tgaagtctgt cttaatgcag
     3241 ctgttcggcg agaacacgga tgtcgagggt atcgacaccc tgaacgcgtg ttacggcggc
     3301 accaacgcac tgttcaatag cctgaactgg attgagagca acgcctggga tggccgcgat
     3361 gcgatcgtcg tgtgcggcga tatcgccatc tatgacaagg gtgcggcacg tccgaccggc
     3421 ggtgcaggca ccgttgcgat gtggattggc ccggacgcac caattgtctt cgattctgtc
     3481 cgcgcgtctt acatggagca cgcctacgac ttttacaagc cggacttcac gagcgaatac
     3541 ccgtacgtgg acggccactt ctctctgacc tgctatgtga aggcgctgga ccaggtttat
     3601 aagtcttata gcaaaaaggc gatttctaag ggcctggtca gcgacccggc aggcagcgac
     3661 gccctgaacg tgctgaagta tttcgactac aacgtgttcc atgtcccgac ctgcaaatta
     3721 gtgaccaaat cttatggccg cctgttatat aatgatttcc gtgccaaccc gcagctgttc
     3781 ccggaggttg acgccgagct ggcgacgcgt gattacgacg agagcctgac cgacaagaac
     3841 atcgagaaga ccttcgtcaa cgtcgcgaag ccgttccaca aagagcgtgt ggcccaaagc
     3901 ctgatcgtcc cgaccaacac gggcaacatg tataccgcgt ctgtctacgc ggcattcgcg
     3961 agcctgctga attacgtcgg ttctgacgac ctgcagggca agcgcgttgg cctgttcagc
     4021 tacggtagcg gcttagcggc cagcctgtat agctgcaaaa ttgtcggcga cgtccagcac
     4081 atcatcaagg agctggacat caccaacaag ctggcgaagc gcatcaccga gacgccgaaa
     4141 gattacgagg cagcgatcga gttacgcgag aatgcgcatc tgaagaagaa cttcaagccg
     4201 caaggtagca tcgagcacct gcagagcggc gtctactacc tgacgaacat tgacgacaag
     4261 ttccgccgtt cttatgacgt caaaaagtaa ctagtaggag gaaaacatca tggtgctgac
     4321 gaacaaaacc gtcattagcg gcagcaaggt gaagtctctg agcagcgccc aaagctctag
     4381 cagcggcccg tctagcagca gcgaggagga cgacagccgt gacattgagt ctctggacaa
     4441 gaagatccgc ccgctggagg agttagaggc cctgctgagc agcggcaaca ccaagcagct
     4501 gaagaacaag gaagttgcag cgctggtgat ccacggtaag ctgccactgt atgcgctgga
     4561 aaagaaactg ggcgatacga cgcgtgcggt cgcggtgcgt cgcaaagcct taagcatctt
     4621 agcggaggcc ccggtgttag ccagcgaccg cctgccgtac aagaactacg actacgaccg
     4681 cgtgtttggc gcgtgctgcg agaatgtcat tggctacatg ccgttaccgg ttggtgtgat
     4741 cggcccgctg gtcattgatg gcacgagcta tcacattcca atggcgacca cggaaggttg
     4801 cttagtcgcc agcgccatgc gtggctgtaa ggcgattaac gccggcggtg gcgcgacgac
     4861 cgtgttaacc aaggatggta tgacgcgcgg tccggtcgtc cgcttcccaa cgctgaagcg
     4921 cagcggcgcg tgtaagattt ggctggattc tgaggagggc caaaacgcga tcaagaaagc
     4981 cttcaactct acgagccgtt tcgcgcgttt acagcatatc cagacctgcc tggccggcga
     5041 cctgctgttc atgcgcttcc gcaccaccac gggcgatgcg atgggcatga acatgatcag
     5101 caagggcgtc gaatatagcc tgaaacaaat ggtggaagaa tatggctggg aggacatgga
     5161 ggttgtctct gtgagcggca actattgcac cgacaagaag ccggcagcca ttaactggat
     5221 tgagggtcgc ggcaaaagcg tcgtggcaga agcgaccatc ccaggcgacg tggtccgtaa
     5281 ggttctgaag agcgacgtca gcgccctggt tgagttaaat atcgcgaaaa acctggtcgg
     5341 cagcgcgatg gcgggcagcg tgggtggctt taacgcacat gcagcgaatc tggttacggc
     5401 ggttttctta gccttaggtc aggacccagc ccaaaatgtc gagagcagca actgcattac
     5461 cttaatgaaa gaggttgacg gtgacctgcg catcagcgtt tctatgccgt ctatcgaggt
     5521 cggcacgatc ggcggcggca ccgttttaga accgcaaggt gcgatgctgg atctgctggg
     5581 cgtgcgcggc ccacatgcaa cggccccagg caccaatgcc cgccaactgg cccgtatcgt
     5641 ggcctgcgcg gttctggcgg gtgagctgag cctgtgcgcc gcattagccg cgggccattt
     5701 agttcaatct cacatgaccc acaaccgcaa gccggcagaa ccaaccaagc caaataacct
     5761 ggacgcaacc gacattaacc gtctgaagga tggcagcgtc acgtgcatta aaagctgatg
     5821 aggatctgga tctaaaggat ctaggaggga gatcatatga gcgaattacg tgcattcagc
     5881 gcgccaggta aggcactgct ggccggtggc tacctggtgt tagacaccaa gtacgaggcg
     5941 ttcgtcgtcg gcttatctgc ccgtatgcat gcagttgccc acccgtatgg tagcctgcag
     6001 ggctctgaca agttcgaagt gcgtgtgaag agcaagcagt tcaaggacgg cgagtggctg
     6061 taccacatta gcccaaagag cggcttcatc ccggttagca ttggtggcag caagaaccca
     6121 tttatcgaga aggtcattgc caacgtcttc agctacttca agccgaatat ggacgattac
     6181 tgcaaccgca acctgttcgt catcgacatt ttcagcgacg acgcgtacca cagccaagag
     6241 gactctgtta cggagcatcg tggtaaccgc cgcctgagct tccacagcca tcgcattgag
     6301 gaggtgccga agacgggtct gggttctagc gccggtttag ttaccgtctt aacgacggcg
     6361 ttagcgagct tcttcgtgag cgacctggag aacaacgtgg acaagtaccg cgaagtgatt
     6421 cataacctgg cgcaggtggc acattgtcag gcccaaggta agattggctc tggttttgat
     6481 gtggcagcgg ccgcctatgg ctctatccgc tatcgccgct ttccgccggc cctgatcagc
     6541 aatctgccgg acatcggctc tgcgacgtat ggtagcaaac tggcgcatct ggtggacgaa
     6601 gaagactgga acatcaccat taagtctaat cacctgccga gcggcttaac gttatggatg
     6661 ggcgatatca agaacggcag cgaaacggtt aagctggtgc agaaagtgaa aaactggtac
     6721 gacagccaca tgccggaaag cctgaagatt tacacggagc tggaccacgc caatagccgt
     6781 ttcatggatg gtctgagcaa gctggaccgc ctgcacgaaa cccacgacga ctacagcgac
     6841 caaatcttcg agagcctgga gcgcaatgac tgcacctgcc agaagtaccc ggagatcacg
     6901 gaggtccgcg atgccgtggc aacgattcgc cgtagcttcc gcaaaattac gaaggagagc
     6961 ggcgcggata tcgaaccacc ggtccagacg tctctgctgg acgactgtca aaccttaaag
     7021 ggcgtgttaa cgtgcctgat tccgggcgcg ggtggttacg acgccattgc cgtcatcacg
     7081 aaacaggacg tcgatctgcg cgcacaaacg gccaacgaca aacgtttcag caaagtccaa
     7141 tggctggatg ttacgcaggc cgactggggt gttcgcaagg agaaggaccc ggaaacgtat
     7201 ctggataagt gaggatctag gaggaaataa ccatgtctct gccattcctg acgtctgcgc
     7261 caggtaaggt gatcatcttc ggcgagcact ctgcggtgta caataagccg gccgtcgccg
     7321 cctctgtgtc tgcgttacgc acctacctgc tgatcagcga atcttctgca ccggacacga
     7381 tcgagctgga ctttccggac atcagcttca accacaagtg gagcatcaac gacttcaacg
     7441 cgatcacgga ggaccaggtg aacagccaaa agctggccaa agcccagcaa gcaaccgacg
     7501 gtctgtctca ggagctggtg tctctgctgg acccgctgtt agcgcagtta agcgagagct
     7561 tccattacca cgccgcgttc tgcttcctgt acatgttcgt ttgcctgtgc ccgcacgcaa
     7621 agaacatcaa gttcagcctg aagagcacgc tgccgattgg cgcaggctta ggctctagcg
     7681 catctatcag cgtgagcctg gcgctggcga tggcctatct gggtggcctg attggcagca
     7741 acgacctgga gaaactgagc gaaaacgaca agcacatcgt gaaccagtgg gcctttatcg
     7801 gcgagaagtg cattcatggc accccgagcg gcattgacaa cgcagttgcc acgtatggca
     7861 acgccctgct gttcgagaaa gacagccaca acggcacgat caacacgaac aacttcaagt
     7921 tcctggacga cttcccggcg atcccgatga ttctgaccta cacccgtatc ccacgcagca
     7981 ccaaggattt agtcgcccgc gtgcgtgttt tagtcaccga aaagttcccg gaggtgatga
     8041 agccgatcct ggacgcgatg ggcgagtgcg cgctgcaggg tctggagatc atgaccaagc
     8101 tgagcaagtg caagggcacc gacgatgagg cggtggaaac caacaatgag ctgtacgagc
     8161 agctgctgga gctgatccgt atcaatcacg gcctgctggt ttctatcggt gtgtctcacc
     8221 cgggcctgga actgatcaaa aacctgagcg acgacctgcg cattggctct acgaaattaa
     8281 cgggtgcagg tggcggtggc tgctctttaa cgctgctgcg ccgtgacatt acgcaggagc
     8341 aaatcgacag cttcaagaag aagctgcagg acgacttcag ctacgagacg ttcgagacgg
     8401 acctgggcgg cacgggctgt tgcctgctga gcgccaaaaa tctgaacaag gacctgaaga
     8461 tcaaaagcct ggtgttccag ctgttcgaaa acaagacgac cacgaagcag cagatcgacg
     8521 acctgttact gccgggtaac accaatctgc cgtggacgtc ttaattagga tccaaactcg
     8581 agtaaggatc tccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     8641 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     8701 tgggcctttc tgcgtttata cctagggata tattccgctt cctcgctcac tgactcgcta
     8761 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
     8821 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
     8881 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
     8941 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
     9001 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
     9061 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
     9121 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9181 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
     9241 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
     9301 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
     9361 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
     9421 agaagatcat cttattaatc agataaaata tttctagatt tcagtgcaat ttatctcttc
     9481 aaatgtagca cctgaagtca gccccatacg atataagttg ttactagtgc ttggattctc
     9541 accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     9601 ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat tacgccccgc
     9661 cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca tggaagccat
     9721 cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat
     9781 aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc acgtttaaat
     9841 caaaactggt gaaactcacc cagggattgg ctgagacgaa aaacatattc tcaataaacc
     9901 ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa tatatgtgta
     9961 gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt tcagtttgct
    10021 catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca
    10081 ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg
    10141 gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa
    10201 cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat
    10261 gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct
    10321 tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat
    10381 ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccag atatc
//
