LOCUS       sequence-173180-       10541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  nuclear split GFP11 reporter with mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10541)
  AUTHORS   Smoyer CJ, Katta SS, Gardner JM, Stoltz L, McCroskey S, Bradford WD,
            McClain M, Smith SE, Slaughter BD, Unruh JR, Jaspersen SL
  TITLE     Analysis of membrane proteins localizing to the inner nuclear 
            envelope in living cells.
  JOURNAL   J Cell Biol. 2016 Nov 21;215(4):575-590. Epub 2016 Nov 9.
  PUBMED    27831485
REFERENCE   2  (bases 1 to 10541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2016-11-21"; volume: "215(4)"; pages: "575-590. Epub 
            2016 Nov 9"
COMMENT     Sequence Label: pSJ1321 (pRS315-NOP1pr-GFP11-mCherry-PUS1)
FEATURES             Location/Qualifiers
     source          1..10541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(42..58)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(42..58)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(55..77)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    66..82
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    135..156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        222..597
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     promoter        789..1004
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             complement(1568..2662)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMSKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTAEVGDAVAEEVKKILA"
     promoter        complement(2663..3070)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(3856..3873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4855..5443)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4935..4954)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5614..6405,6406..6474))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6237..6256
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6475..6579)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6647..6665
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6703..6725)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7038..7060
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7052..7069
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7053..7069
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7100..7116
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7100..7116
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             7768..7815
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
ORIGIN
        1 ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
       61 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      181 ttccagtcgg gaaacctgtc gtgccagcag atctgaatta attcttgaat aatacataac
      241 ttttcttaaa agaatcaaag acagataaaa tttaagagat attaaatatt agtgagaagc
      301 cgagaatttt gtaacaccaa cataacactg acatctttaa caacttttaa ttatgataca
      361 tttcttacgt catgattgat tattacagct atgctgacaa atgactcttg ttgcatggct
      421 acgaaccggg taatactaag tgattgactc ttgctgacct tttattaaga actaaatgga
      481 caatattatg gagcatttca tgtataaatt ggtgcgtaaa atcgttggat ctctcttcta
      541 agtacatcct actataacaa tcaagaaaaa caagaaaatc ggacaaaaca atcaagtatg
      601 gattctagaa cagttggtat attaggaggg ggacaattgg gacgtatgat tgttgaggca
      661 gcaaacaggc tcaacattaa gacggtaata ctagatgctg aaaattctcc tgccaaacaa
      721 ataagcaact ccaatgacca cgttaatgca tcagagcaga ttgtactgag agtgcaccat
      781 accacctttt caattcatca tttttttttt attctttttt ttgatttcgg tttccttgaa
      841 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta
      901 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa
      961 cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat
     1021 ataaggaacg tgctgctact catcctagtc ctgttgctgc caaggcagat tgtactgaga
     1081 gtgcaccata tcgactacgt cgttaaggcc gtttctgaca gagtaaaatt cttgagggaa
     1141 ctttcaccat tatgggaaat ggttcaagaa ggtattgact taaactccat caaatggtca
     1201 ggtcattgag tgttttttat ttgttgtatt tttttttttt tagagaaaat cctccaatat
     1261 ataaattagg aatcatagtt tcatgatttt ctgttacacc taactttttg tgtggtgccc
     1321 tcctcctcgt caatattaat gttaaagtgc aattcttttt ccttatcacg ttgagccatt
     1381 agtatcaatt tgcttacctg tattccttta catcctcctt tttctccttc ttgataaatg
     1441 tatgtagatt gcgtatatag tttcgtctac cctatgaaca tattccattt tgtaatttcg
     1501 tgtcgtttct attatgaatt tcatttataa agtttatgta caaatatcat aaaaaaagag
     1561 aatcttttta agcaaggatt ttcttaactt cttcggcgac agcatcaccg acttcggcgg
     1621 tactgttgga accacctaaa tcaccagttc tgatacctgc atccaaaacc tttttaactg
     1681 catcttcaat ggccttacct tcttcaggca agttcaatga caatttcgac atcattgcag
     1741 cagacaagat agtggcgata gggttgacct tattctttgg caaatctgga gcagaaccgt
     1801 ggcatggttc gtacaaacca aatgcggtgt tcttgtctgg caaagaggcc aaggacgcag
     1861 atggcaacaa acccaaggaa cctgggataa cggaggcttc atcggagatg atatcaccaa
     1921 acatgttgct ggtgattata ataccattta ggtgggttgg gttcttaact aggatcatgg
     1981 cggcagaatc aatcaattga tgttgaacct tcaatgtagg gaattcgttc ttgatggttt
     2041 cctccacagt ttttctccat aatcttgaag aggccaaaac attagcttta tccaaggacc
     2101 aaataggcaa tggtggctca tgttgtaggg ccatgaaagc ggccattctt gtgattcttt
     2161 gcacttctgg aacggtgtat tgttcactat cccaagcgac accatcacca tcgtcttcct
     2221 ttctcttacc aaagtaaata cctcccacta attctctgac aacaacgaag tcagtacctt
     2281 tagcaaattg tggcttgatt ggagataagt ctaaaagaga gtcggatgca aagttacatg
     2341 gtcttaagtt ggcgtacaat tgaagttctt tacggatttt tagtaaacct tgttcaggtc
     2401 taacactacc ggtaccccat ttaggaccac ccacagcacc taacaaaacg gcatcagcct
     2461 tcttggaggc ttccagcgcc tcatctggaa gtggaacacc tgtagcatcg atagcagcac
     2521 caccaattaa atgattttcg aaatcgaact tgacattgga acgaacatca gaaatagctt
     2581 taagaacctt aatggcttcg gctgtgattt cttgaccaac gtggtcacct ggcaaaacga
     2641 cgatcttctt aggggcagac attagaatgg tatatccttg aaatatatat atatatattg
     2701 ctgaaatgta aaaggtaaga aaagttagaa agtaagacga ttgctaacca cctattggaa
     2761 aaaacaatag gtccttaaat aatattgtca acttcaagta ttgtgatgca agcatttagt
     2821 catgaacgct tctctattct atatgaaaag ccggttccgg cgctctcacc tttccttttt
     2881 ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg acctctgaaa ttaacaaaaa
     2941 atttccagtc atcgaatttg attctgtgcg atagcgcccc tgtgtgttct cgttatgttg
     3001 aggaaaaaaa taatggttgc taagagattc gaactcttgc atcttacgat acctgagtat
     3061 tcccacagtt aactgcggtc aagatatttc ttgaatcagg cgccttagac cgctcggcca
     3121 aacaaccaat tacttgttga gaaatagagt ataattatcc tataaatata acgcttttga
     3181 acacacatga acagggaagt acaggacaat tgattttgaa gagaatgtgg attttgatgt
     3241 aattgttggg attccatttt taataaggca ataatattag gtatgtagat atactagaag
     3301 ttctcctcga ccggtcgata tgcggtgtga aataccgcac agagggtgaa cgttacagaa
     3361 aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat
     3421 aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat
     3481 taccctatgc ggtgtgaaat accgcacaga tgcgtaggag attcaagtta tacaacgaaa
     3541 atggaacagt ttttattaaa gcaagaagaa gaagttcttg tcaaagcaca aaagttagaa
     3601 actgtcggtt acgaagctta tctagaaaac aagtaatata taagtttatt gatatacttg
     3661 tacagcaaat aattataaaa tgatatacct attttttagg ctttgttatg attacatcaa
     3721 atgtggactt catacataga aatcaacgct tacaggtgtc cttttttaag aatttcatac
     3781 ataagatctg ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     3841 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     3901 ggtatcagca tcgatgctag agtcgaatgg aaattacgta ctttcaacgc aagagcaaga
     3961 caaaagaaag cctaaaactg tcttttcgtg tcggtcgtcc aatatcatcg taaacgtgtg
     4021 tagagcctta aattctaatt tctaacttta aatttttttt gctattataa tactaatttc
     4081 taactttcaa cctattttac atcttcggaa aacaaaaact attttttctt taatttcttt
     4141 ttttactttc tatttttaat ttatatattt atattaaaaa atttaaatta taattatttt
     4201 tatagcacgt gatgaaaaga aatatagtct tcaaggttat attatacagg tttatatatt
     4261 attttactta tttttgttgt tctttttctt ggagtattta gcattaacaa cttcgaccca
     4321 tcgcgatgac gaaattgcac gtttaaagga gaagtcggaa tcgttgcaag aagaaataaa
     4381 ttagtttgac gttttattcc ttttcttctg cacagaagtg tgtatatata tatttatata
     4441 tagtcgatgt gcaaaagcct acattatttt tcttttgatt tttatttttt tgttcaagta
     4501 ggttcatgcc gacagtaaat ccttatgccc tacctaaata taaacaccca aatatatata
     4561 tatatatata tatatataaa taataaaaac ggaaaggaaa tagaataata ataataatac
     4621 tacacgttta cgtatgtaac tcattctatt attcagactt tgatattaat agaattatta
     4681 ctactaggtt tcctcacttc aaaatctata aatataccgt catgctctaa tcgatgctca
     4741 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4801 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4861 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4921 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4981 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5041 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5101 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5161 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5221 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5281 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5341 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5401 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5461 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5521 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5581 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5641 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5701 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5761 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5821 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5881 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5941 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     6001 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     6061 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6121 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6181 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6241 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6301 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6361 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6421 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6481 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6541 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6601 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6661 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     6721 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     6781 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     6841 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
     6901 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     6961 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     7021 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct
     7081 ccaccgcggt ggcggccgct ctagaactag tggatccatt gagtcatcag cctcttcttg
     7141 aaaaaacgat tctgaaataa ccggaacttg tttagattcc tttttgtagg ttaatcttat
     7201 cctgtacttg ctgatattgt ggttattagc agagattttt ttcaaaacat catctaaagt
     7261 aggctttttg gataagtcaa tttcagtgtc ccttaaccct ttagagcggc tttttatggt
     7321 gataggcatt ttcaaattaa attcaaaata tgtatctctc tcaaatagct caattggttt
     7381 ctagatagct aaatagaata taatcttact gtcctccgtt ctgtaaaatt cacgctctta
     7441 gtcccttttc ataattcctt aactttttgc gtacaaaatg atatgtttat tatatttttc
     7501 tttttttttt ttcaaatttt ttctttttct tgaaaaattt ttcaaattgg aaagctcatc
     7561 tctcttgaat gtataatact ttcttcctct aactttcaaa aagttttaca tagccaagaa
     7621 gttttcctta catcggtata ctactgttat ataagttatt cttcgagaaa caattagata
     7681 tcattcatcg gataaatcta agttgcccat tgctttcaat aactccgatc aaattaactc
     7741 aaatcaacta aaacagtagg gcccatgaga gatcatatgg ttttgcatga atatgttaat
     7801 gctgctggta ttactggtgg tggttctggt ggtggttctc tcgagatggt ttctaaaggt
     7861 gaagaagata atatggctat tattaaagaa tttatgagat ttaaagttca tatggaaggt
     7921 tctgttaatg gtcatgaatt tgaaattgaa ggtgaaggtg aaggtagacc atatgaaggt
     7981 actcaaactg ctaaattgaa agttactaaa ggtggtccat tgccatttgc ttgggatatt
     8041 ttgtctccac aatttatgta tggttctaaa gcttatgtta aacatccagc tgatattcca
     8101 gattatttga aattgtcttt tccagaaggt tttaaatggg aaagagttat gaattttgaa
     8161 gatggtggtg ttgttactgt tactcaagat tcttctttgc aagatggtga atttatttat
     8221 aaagttaaat tgagaggtac taattttcca tctgatggtc cagttatgca aaaaaaaact
     8281 atgggttggg aagcttcttc tgaaagaatg tatccagaag atggtgcttt gaaaggtgaa
     8341 attaaacaaa gattgaaatt gaaagatggt ggtcattatg atgctgaagt taaaactact
     8401 tataaagcta aaaaaccagt tcaattgcca ggtgcttata atgttaatat taaattggat
     8461 attacttctc ataatgaaga ttatactatt gttgaacaat atgaaagagc tgaaggtaga
     8521 cattctactg gtggtatgga tgaattgtat aaaggtggtg gtgctagcat gtctgaagag
     8581 aatttgaggc ctgcctatga cgatcaagtt aatgaggatg tctacaagag aggcgctcag
     8641 agcaaattga ccaaagctcg taaggcagac ttcgacgatg aaaaggacaa aaaaaaggat
     8701 aatgacaaac acatagataa aagaccaaaa agcggtcctc gtttggatga aaatggcaat
     8761 ccacttccca aggagcctcg tctcccaaag agaaaggtgg ctgttatggt tggttactgt
     8821 ggtactggct accatggtat gcaatataat ccaccaaatc caaccatcga gtctgcccta
     8881 ttcaaagcat ttgttgaagc aggtgctatt tccaaggaca actccaacga tttgaaaaag
     8941 aatggattca tgagagctgc aagaactgat aagggtgtcc atgctggagg taatttaata
     9001 tcattgaaaa tgattattga agatcctgat atcaagcaga agatcaacga aaagttaccg
     9061 gaagggataa gggtatggga tatcgagcgt gtgaacaagg cgtttgattg tagaaaaatg
     9121 tgcagttcac gttggtacga atacttatta ccaacttatt cactcattgg ccccaaacct
     9181 ggttctatac tttatagaga catcgaagaa agtaaaacag aattacctgg tgtattagat
     9241 gaagatttgg aatccaaaga attttgggaa gagtttaaaa aagatgcgaa tgaaaaattt
     9301 tcaacagaag aaatcgaagc tatattagca tatgttccgc ccgcaagaga tgaatttgat
     9361 ataaatgaag aactatacca gaaggtaaaa aagtacaagc aattagaaaa tgctcaccgt
     9421 agaagatatc gtatatccgc ggcaaaactg gccaaattcc gtgcttctac aagtcaatac
     9481 cttggcgctc ataacttcca taattttaca ttaggcaagg acttcaaaga accaagtgcc
     9541 attcgattta tgaaagatat aaaagtatct gatccatttg ttattggcga tgcccaaaca
     9601 gaatggattt ccatcaagat tcacggccag tctttcatgc tacatcaaat tcgtaagatg
     9661 gtatcaatgg caaccttaat tacccgttgt ggttgccccg tcgaacgcat tagtcaggct
     9721 tacggacagc agaagattaa tattccgaag gcacctgcat taggattatt gttagaagct
     9781 ccagtatttg aaggttataa taaaagacta gaacagtttg gttacaaggc gatcgatttt
     9841 tccaaatatc aagacgaagt ggataagttc aaaatgaagc atatctacga taagatctac
     9901 aaggaagagg tggacgaaaa tgtttttaat gcgttcttta gttatattga tagtttcaac
     9961 aaggttactg gagcacaggg agaagaaact gccgataaat cgggacctgc tgtgcaaaag
    10021 agtatcttcg aattcttgac agctaagggt atcccaggtc ttactgatgc gcccgagagc
    10081 aataagaaga ttaagcaaag aaaaagaatg gaagaggagg aagctgctag taaaaaggct
    10141 gaaatatcca gcaccactca gagcaacgaa cctgaagtac aaccggaagc ggcagctaat
    10201 taggttgccc tttccttctt tcttaccaac tttaatttct aaggcattga cattgaaatt
    10261 gacattgtcc tctaatgttt atagagcacc attatttgtt ttcgttttat atattattat
    10321 ataatggcac agaataattt ataattcttt ttttttaagt cattgaacag tcgcactaac
    10381 caaaagatta aacaccacca gagagtagtt tttccagcct ggcaatctct tctaaagaag
    10441 tagcttctgc taactgcttt ttcaattcat ttcttctctc tactgtcatc ttactcacca
    10501 ccaaattcat gatctccatc gttttatctc tggatccttg t
//
