LOCUS       sequence-173182-        6963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for 3' FLAG tag of human ZNF792.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts 
            BS, Mackiewicz M, Mendenhall EM, Myers RM
  TITLE     CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
  JOURNAL   Genome Res. 2015 Sep 9.
  PUBMED    26355004
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2015 Sep 9."
COMMENT     Sequence Label: pFETCh_ZNF792
FEATURES             Location/Qualifiers
     source          1..6963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      10..598
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     499..518
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     752..769
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1069..1090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1105..1110,1111..1128,1129..1135)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1143..1159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1148..1170
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1167..1183
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1167..1183
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1360..1562
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1513..1533
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1559..1583
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1659..2789
                     /codon_start=1
                     /product="herpes simplex virus thymidine kinase"
                     /label=HSV TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             3816..3839
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3843..3866
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3870..3893
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3909..3965
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3978..4772
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4032..4051)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4642..4661
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5543..5559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5552..5574)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(5778..6593)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNILQFHLMLDEFF"
     primer_bind     6501..6520
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6585..6604
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
       61 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      121 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      181 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      241 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      301 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      361 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      421 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      481 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      541 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      601 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      661 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
      721 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      781 agctcgcaca ttcagcagcg tttttcagcg cgttttcgat caacgtttca atgttggtat
      841 caacaccagg tttaactttg aacttatcgg cactgacggt tactgatttt gaacttttgc
      901 tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca
      961 aaagttccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     1021 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     1081 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     1141 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct
     1201 tgcatgcctg caggtcgact ctagaggatc cccgggtacc gagctcgaat tagcttgcat
     1261 gcctgcaggt caattccctg gcattatgcc cagtacatga ccttatggga ctttcctact
     1321 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     1381 atcaatgggc gtggatagcg gtttgactca cggggatttc aagtctccac cccattgacg
     1441 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     1501 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     1561 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1621 ggagacaccg ggaccgatcc agcctgggga tcccgaccat ggcttcgtac ccctgccatc
     1681 aacacgcgtc tgcgttcgac caggctgcgc gttctcgcgg ccatagcaac cgacgtacgg
     1741 cgttgcgccc tcgccggcag caagaagcca cggaagtccg cctggagcag aaaatgccca
     1801 cgctactgcg ggtttatata gacggtcctc acgggatggg gaaaaccacc accacgcaac
     1861 tgctggtggc cctgggttcg cgcgacgata tcgtctacgt acccgagccg atgacttact
     1921 ggcaggtgct gggggcttcc gagacaatcg cgaacatcta caccacacaa caccgcctcg
     1981 accagggtga gatatcggcc ggggacgcgg cggtggtaat gacaagcgcc cagataacaa
     2041 tgggcatgcc ttatgccgtg accgacgccg ttctggctcc tcatatcggg ggggaggctg
     2101 ggagctcaca tgccccgccc ccggccctca ccctcatctt cgaccgccat cccatcgccg
     2161 ccctcctgtg ctacccggcc gcgcgatacc ttatgggcag catgaccccc caggccgtgc
     2221 tggcgttcgt ggccctcatc ccgccgacct tgcccggcac aaacatcgtg ttgggggccc
     2281 ttccggagga cagacacatc gaccgcctgg ccaaacgcca gcgccccggc gagcggcttg
     2341 acctggctat gctggccgcg attcgccgcg tttacgggct gcttgccaat acggtgcggt
     2401 atctgcaggg cggcgggtcg tggcgggagg attggggaca gctttcgggg acggccgtgc
     2461 cgccccaggg tgccgagccc cagagcaacg cgggcccacg accccatatc ggggacacgt
     2521 tatttaccct gtttcgggcc cccgagttgc tggcccccaa cggcgacctg tacaacgtgt
     2581 ttgcctgggc cttggacgtc ttggccaaac gcctccgtcc catgcacgtc tttatcctgg
     2641 attacgacca atcgcccgcc ggctgccggg acgccctgct gcaacttacc tccgggatgg
     2701 tccagaccca cgtcaccacc cccggctcca taccgacgat ctgcgacctg gcgcgcacgt
     2761 ttgcccggga gatgggggag gctaactgag gatcccacgt cactattgta tactctatat
     2821 tatactctat gttatactct gtaatcctac tcaataaacg tgtcacgcct gtgaaaccgt
     2881 actaagtctc ccgtgtattc ttatcaccat caggtgacat cctcgcccag gctgtcaatc
     2941 atgccggtat cgattccagt agcaccggcc ccacgctgac aacccactct tgcagcgtta
     3001 gcagcgcccc tcttaacaag ccgaccccca ccagcgtcgc ggttactaac actcctctcc
     3061 ccgacctgca gcccagctgt gggaaatctt tcaactgcaa ctccagccta attaaacatt
     3121 ggagagttca cactggagaa agaccttaca agtgtaacga atgtgggaaa ttcttcagcc
     3181 acattgccag cctcattcaa catcagatag ttcacactgg cgagcggcct cacgggtgtg
     3241 gtgagtgtgg gaaagccttc agccgaagct ctgacctcat gaaacatcag cgagttcaca
     3301 ctggtgagcg gccttatgaa tgcaatgaat gtgggaagtt atttagccag agctccagcc
     3361 tcaatagcca tcggagactt cacactggtg aacggcctta ccagtgcagt gaatgtggga
     3421 aattctttaa ccaaagctcc agcctcaata accaccggag acttcacacc ggcgagcggc
     3481 cttatgagtg cagcgaatgt gggaaaacct tcaggcagag gtccaatctg aggcagcacc
     3541 tgaaagttca caaaccagac aggccttacg aatgcagcga atgtgggaaa gccttcaacc
     3601 aaaggcctac cctcattcgg catcagaaga ttcacatcag agaaaggagc atggagaatg
     3661 tgctccttcc ctgttcacag cacacaccag agataagctc tgagaacaga ccttatcagg
     3721 gcgctgtcaa ctacaagttg aaacttgttc atccaagtac ccaccctggg gaggttcccg
     3781 ggagcggagg aggttccggt ggaggtggtt ctggagatta caaggatgac gacgataagg
     3841 gcgattacaa ggatgacgac gataagggag attacaagga tgacgacgat aaggtttcag
     3901 gaagcggagc tactaacttc agcctgctga agcaggctgg agacgtggag gagaaccctg
     3961 gacctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     4021 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     4081 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     4141 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     4201 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     4261 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     4321 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     4381 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     4441 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     4501 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     4561 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     4621 ccgaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     4681 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     4741 ccttctatcg ccttcttgac gagttcttct gattcgaaca tcaatgctag ctgtgttgga
     4801 agctttctgg agacaagtta cattctctta ctgtagagtt tatcagcgtt ttttcactgc
     4861 tggggttgtg atagaagcca tgtcagcacc acacactgca gctctccaaa gagtgtgtcc
     4921 accactccac tgtgctcagg gaaggcagac ctctcctctc tctttccaat ccctaaaggg
     4981 aattaggagt agtctgaagc cttgggaaga tgtcattccc gccctgtatg gctggtttag
     5041 ccatggacat gaccagcttt tggctgtgaa gacctgagca gggttttgcc aacagcttgt
     5101 tctagagaag gtttcccttt ttctgggaca ttgttggtct catgttatgc ctgtgaagga
     5161 tttgtccagc cttcatgtta ctctacacaa caacttatct cctgtgtatt gccctggttc
     5221 atgtgatgat aatggcctta tgataaagcc gcctttagtc ttccatgttc tgatcactgt
     5281 gtggggtggt tcaagagcag agttaagatc taccaaactg aaggggtctc cagattatct
     5341 tggaggggac aggaggatgg cagagaaaca actgtcaatc cagatttgac ctcatttgca
     5401 ttgccaccaa ggcctcctag gaaaaattgt aggaatttat gggtataata ttgtggtctg
     5461 aatggcgtga atttttatcc cgaggagggg cttggaaagt cctctccact gactgtagcc
     5521 tccaattcac tggccgtcgt tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     5581 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     5641 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgcg
     5701 atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac
     5761 caattaacca attctgatta gaaaaactca tcgagcatca aatgaaactg caatatattc
     5821 atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac
     5881 tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt
     5941 ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa
     6001 tcaccatgag tgacgactga atccggtgag aatggcaaaa gcttatgcat ttctttccag
     6061 acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg
     6121 ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa
     6181 ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt
     6241 tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg
     6301 gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata
     6361 aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct
     6421 ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc
     6481 gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg
     6541 ttggaattta atcgcggctt cgagcaagac gtttcccgtt gaatatggct cataacaccc
     6601 cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct
     6661 tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact
     6721 tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca
     6781 aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc
     6841 tccctcactt tctggctgga tgatggggcg attcaggcct ggtatgagtc agcaacacct
     6901 tcttcacgag gcagacctct cgacggagtt ccactgagcg tcagaccccg tagaaaagat
     6961 caa
//
