LOCUS       sequence-173187-        7812 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Focal Adhesion Kinase, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7812)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEos2-PTK2-N-10
FEATURES             Location/Qualifiers
     source          1..7812
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3196..3876
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    3998..4119
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4035..4054)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4089..4108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4126..4581)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4263..4284)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4475..4494
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4608..4712
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4714..5071
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4714..4905
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4737..4757)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4922..5057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5106..5900
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5106..5900
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5160..5179)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5770..5789
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6088..6107)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6132..6179
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6508..7096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6997..7016
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7271..7574
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7575..7778
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7729..7749
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      join(7807..7812,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggcagctg cttaccttga cccccacttg aatcacacac caaattcgag tactaagact
       61 cacctgggta ctggtatgga acgttctcct ggtgcaatgg agcgagtatt aaaggtcttt
      121 cattattttg aaagcaatag tgagccaacc acctgggcca gtattatcag gcatggagat
      181 gctactgatg tcaggggcat cattcagaag atagtggaca gtcacaaagt aaagcatgtg
      241 gcctgctatg gattccgcct cagtcacctg cggtcagagg aggttcactg gcttcacgtg
      301 gatatgggcg tctccagtgt gagggagaag tatgagcttg ctcacccacc agaggagtgg
      361 aaatatgaat tgagaattcg ttatttgcca aaaggatttc taaaccagtt tactgaagat
      421 aagccaactt tgaatttctt ctatcaacag gtgaagagcg attatatgtt agagatagct
      481 gatcaagtgg accaggaaat tgctttgaag ttgggttgtc tagaaatacg gcgatcatac
      541 tgggagatgc ggggcaatgc actagaaaag aagtctaact atgaagtatt agaaaaagat
      601 gttggtttaa agcgattttt tcctaagagt ttactggatt ctgtcaaggc caaaacacta
      661 agaaaactga tccaacaaac atttagacaa tttgccaacc ttaatagaga agaaagtatt
      721 ctgaaattct ttgagatcct gtctccagtc tacagatttg ataaggaatg cttcaagtgt
      781 gctcttggtt caagctggat tatttcagtg gaactggcaa tcggcccaga agaaggaatc
      841 agttacctaa cggacaaggg ctgcaatccc acacatcttg ctgacttcac tcaagtgcaa
      901 accattcagt attcaaacag tgaagacaag gacagaaaag gaatgctaca actaaaaata
      961 gcaggtgcac ccgagcctct gacagtgacg gcaccatccc taaccattgc ggagaatatg
     1021 gctgacctaa tagatgggta ctgccggctg gtgaatggaa cctcgcagtc atttatcatc
     1081 agacctcaga aagaaggtga acgggctttg ccatcaatac caaagttggc caacagcgaa
     1141 aagcaaggca tgcggacaca cgccgtctct gtgtcagaaa cagatgatta tgctgagatt
     1201 atagatgaag aagatactta caccatgccc tcaaccaggg attatgagat tcaaagagaa
     1261 agaatagaac ttggacgatg tattggagaa ggccaatttg gagatgtaca tcaaggcatt
     1321 tatatgagtc cagagaatcc agctttggcg gttgcaatta aaacatgtaa aaactgtact
     1381 tcggacagcg tgagagagaa atttcttcaa gaagccttaa caatgcgtca gtttgaccat
     1441 cctcatattg tgaagctgat tggagtcatc acagagaatc ctgtctggat aatcatggag
     1501 ctgtgcacac ttggagagct gaggtcattt ttgcaagtaa ggaaatacag tttggatcta
     1561 gcatctttga tcctgtatgc ctatcagctt agtacagctc ttgcatatct agagagcaaa
     1621 agatttgtac acagggacat tgctgctcgg aatgttctgg tgtcctcaaa tgattgtgta
     1681 aaattaggag actttggatt atcccgatat atggaagata gtacttacta caaagcttcc
     1741 aaaggaaaat tgcctattaa atggatggct ccagagtcaa tcaattttcg acgttttacc
     1801 tcagctagtg acgtatggat gtttggtgtg tgtatgtggg agatactgat gcatggtgtg
     1861 aagccttttc aaggagtgaa gaacaatgat gtaatcggtc gaattgaaaa tggggaaaga
     1921 ttaccaatgc ctccaaattg tcctcctacc ctctacagcc ttatgacgaa atgctgggcc
     1981 tatgacccca gcaggcggcc caggtttact gaacttaaag ctcagctcag cacaatcctg
     2041 gaggaagaga aggctcagca agaagagcgc atgaggatgg agtccagaag acaggccaca
     2101 gtgtcctggg actccggagg gtctgatgaa gcaccgccca agcccagcag accgggttat
     2161 cccagtccga ggtccagcga aggattttat cccagcccac agcacatggt acaaaccaat
     2221 cattaccagg tttctggcta ccctggttca catggaatca cagccatggc tggcagcatc
     2281 tatccaggtc aggcatctct tttggaccaa acagattcat ggaatcatag acctcaggag
     2341 atagcaatgt ggcagcccaa tgtggaggac tctacagtat tggacctgcg agggattggg
     2401 caagtgttgc caacccatct gatggaagag cgtctaatcc gacagcaaca ggaaatggaa
     2461 gaagatcagc gctggctgga aaaagaggaa agatttctga aacctgatgt gagactctct
     2521 cgaggcagta ttgacaggga ggatggaagt cttcagggtc cgattggaaa ccaacatata
     2581 tatcagcctg tgggtaaacc agatcctgca gctccaccaa agaaaccgcc tcgccctgga
     2641 gctcccggtc atctgggaag ccttgccagc ctcagcagcc ctgctgacag ctacaacgag
     2701 ggtgtcaagc catggaggct tcagccccag gaaatcagcc cccctcctac tgccaacctg
     2761 gaccggtcga atgataaggt gtacgagaat gtgacgggcc tggtgaaagc tgtcatcgag
     2821 atgtccagta aaatccagcc agccccacca gaggagtatg tccctatggt gaaggaagtc
     2881 ggcttggccc tgaggacatt attggccact gtggatgaga ccattcccct cctaccagcc
     2941 agcacccacc gagagattga gatggcacag aagctattga actctgacct gggtgagctc
     3001 atcaacaaga tgaaactggc ccagcagtat gtcatgacca gcctccagca agagtacaaa
     3061 aagcaaatgc tgactgctgc tcacgccctg gctgtggatg ccaaaaactt actcgatgtc
     3121 attgaccaag caagactgaa aatgcttggg cagacgagac cacacagctc cggaggggat
     3181 ccaccggtcg ccaccatgag tgcgattaag ccagacatga agatcaaact ccgtatggaa
     3241 ggcaacgtaa acgggcacca ctttgtgatc gacggagatg gtacaggcaa gccttttgag
     3301 ggaaaacaga gtatggatct tgaagtcaaa gagggcggac ctctgccttt tgcctttgat
     3361 atcctgacca ctgcattcca ttacggcaac agggtattcg ccaaatatcc agacaacata
     3421 caagactatt ttaagcagtc gtttcctaag gggtattcgt gggaacgaag cttgactttc
     3481 gaagacgggg gcatttgcat tgccagaaac gacataacaa tggaagggga cactttctat
     3541 aataaagttc gattttatgg taccaacttt cccgccaatg gtccagttat gcagaagaag
     3601 acgctgaaat gggagccctc cactgagaaa atgtatgtgc gtgatggagt gctgacgggt
     3661 gatattcata tggctttgtt gcttgaagga aatgcccatt accgatgtga cttcagaact
     3721 acttacaaag ctaaggagaa gggtgtcaag ttaccaggct accactttgt ggaccactgc
     3781 attgagattt taagccatga caaagattac aacaaggtta agctgtatga gcatgctgtt
     3841 gctcattctg gattgcctga caatgccaga cgataagcgg ccgcgactct agatcataat
     3901 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     3961 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     4021 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4081 ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta
     4141 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     4201 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
     4261 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     4321 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
     4381 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     4441 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     4501 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     4561 atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4621 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4681 aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa
     4741 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     4801 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     4861 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     4921 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4981 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5041 aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt
     5101 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     5161 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     5221 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     5281 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     5341 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     5401 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     5461 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     5521 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     5581 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc
     5641 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     5701 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     5761 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     5821 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     5881 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc
     5941 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg
     6001 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt
     6061 cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac
     6121 ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca
     6181 taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg
     6241 gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg
     6301 cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata
     6361 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6421 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6481 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6541 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6601 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6661 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6721 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6781 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6841 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6901 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6961 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     7021 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7081 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7141 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7201 cgccatgcat tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     7261 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     7321 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     7381 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
     7441 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     7501 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     7561 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     7621 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     7681 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     7741 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
     7801 gctagcgcca cc
//
