LOCUS       sequence-173193-        8076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Cadherin, Excitation: 505 / 569, Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8076)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 8076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-Desmocollin1-N-18
FEATURES             Location/Qualifiers
     source          1..8076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2737..3414
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             3463..4140
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     polyA_signal    4262..4383
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4299..4318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4353..4372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4390..4845)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4527..4548)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4739..4758
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4872..4976
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4978..5335
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4978..5169
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5001..5021)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5186..5321
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5370..6164
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5370..6164
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5424..5443)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6034..6053
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6352..6371)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6396..6443
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6772..7360
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7261..7280
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7535..7838
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7839..8042
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7993..8013
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      join(8071..8076,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggctctgg cctctgctgc cccagggagc atcttctgta agcagctcct tttctctctc
       61 ctggttttaa cattactttg cgatgcttgt cagaaagttt atcttcgagt tccttctcat
      121 cttcaggctg aaacacttgt aggcaaagtg aatctggagg agtgtctcaa gtcggccagc
      181 ctaatccggt ccagtgaccc tgccttcagg attctagaag atggctcaat ttacacaaca
      241 catgacctca ttttgtcttc tgaaaggaaa agtttttcca ttttcctttc agatggtcag
      301 agacgggaac aacaagagat aaaagttgta ctgtcagcaa gagaaaacaa gtctcctaag
      361 aagagacata ccaaagacac agccctcaag cgcagcaaga gacgatgggc tcctattcca
      421 gcttcattga tggagaactc gttgggtcca tttccacaac acgttcagca gatccaatct
      481 gatgctgcac agaattacac catcttttat tccataagtg ggccaggcgt ggacaaagaa
      541 cccttcaatt tgttttacat agagaaagac actggggata tcttttgtac aaggagcatt
      601 gaccgtgaga aatacgaaca gtttgcgtta tatggctatg caacaactgc agatggctat
      661 gcaccagaat atccactccc tttgatcatc aaaattgaag atgataatga taacgcccca
      721 tattttgaac acagagtgac tatctttact gtgcctgaaa attgccgatc cggcacttca
      781 gtgggaaaag tgaccgccac agaccttgac gaacctgaca ctctccatac tcgtctgaaa
      841 tataaaatct tacaacaaat cccagatcat ccaaagcatt tctccataca cccagatacc
      901 ggagtcatca ccacaactac accttttctg gatagagaaa aatgtgatac ttaccagtta
      961 ataatggaag tgcgagacat gggtggtcag cctttcggtt tatttaatac aggaacaatt
     1021 actatttcac ttgaggatga aaatgacaat ccaccatctt tcacagaaac ttcttatgtt
     1081 acagaagtag aagaaaacag aattgacgtg gagattttgc gaatgaaggt acaggatcag
     1141 gatttgccaa acactcctca ctcaaaggct gtatacaaaa tcttacaagg aaatgaaaat
     1201 ggaaacttca taattagcac agatccaaat acaaatgaag gagtgctgtg tgttgtcaag
     1261 ccattgaact atgaagtcaa tcgccaagtt attttgcaag ttggtgtcat taacgaggca
     1321 caattctcta aagcagcgag ctcacaaact cctacaatgt gcactacaac tgtcaccgtt
     1381 aaaattatag acagtgatga gggccctgaa tgccaccctc cagtgaaagt tattcagagt
     1441 caagatggct tcccagctgg ccaagaactc cttggataca aagcactgga cccggaaata
     1501 tccagtggtg aaggcttaag gtatcagaag ttaggggatg aagataactg gtttgaaatt
     1561 aatcaacaca ctggcgactt gagaactcta aaagtactag atagagaatc caaatttgta
     1621 aaaaacaacc aatacaatat ttcagttgtt gcagtggatg cagttggccg atcttgcact
     1681 ggaacattag tagttcattt ggatgattac aacgatcacg cacctcaaat tgacaaagaa
     1741 gtgaccattt gtcagaataa tgaggatttt gctgttctga aacctgtaga tccagatgga
     1801 cctgaaaatg gaccaccttt tcaattcttt ctggataatt ctgccagtaa aaactggaac
     1861 atagaagaaa aggatggtaa aactgccatt cttcgtcaac ggcaaaatct tgattataac
     1921 tattattctg tgcctattca aataaaagac aggcatggtt tagttgcaac acatatgtta
     1981 acagtgagag tatgtgactg ttcaactcca tctgagtgta gaatgaagga taaaagtaca
     2041 agagacgtta gaccaaatgt aatacttgga agatgggcta ttcttgctat ggtgttgggt
     2101 tctgtattgt tattatgtat tctgtttaca tgtttctgtg tcactgctaa gagaacagtc
     2161 aagaaatgtt ttccagaaga catagcccag caaaatttaa ttgtatcaaa tactgaagga
     2221 cctggagaag aagtaacgga agcaaatatt agactcccca tgcagacatc caacatttgt
     2281 gacacaagca tgtctgttgg tactgttggt ggccagggaa tcaaaacaca gcaaagtttt
     2341 gagatggtca aaggaggcta cactttggat tccaacaaag gaggtggaca tcagaccttg
     2401 gagtccgtca agggagtggg gcagggagat actggcagat atgcgtacac ggactggcag
     2461 agtttcaccc aacctcggct tggcgaaaag gtgtatttgt gtggacaaga tgaggagcat
     2521 aaacattgtg aagactacgt ttgttcgtat aactatgaag gcaaaggttc tctggccggc
     2581 tcagtaggtt gctgcagcga tcggcaggaa gaagagggac tggagtttct agatcacctg
     2641 gaacccaaat ttaggacatt agcaaagaca tgcatcaaga aaagcagcgg tggagcaagc
     2701 gcagccagtg gtagcgcgga tccaccggtc gccaccatga gtgcgattaa gccagacatg
     2761 aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat cgacggagat
     2821 ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa agagggcgga
     2881 cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa cagggtattc
     2941 gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa ggggtattcg
     3001 tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa cgacataaca
     3061 atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt tcccgccaat
     3121 ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa aatgtatgtg
     3181 cgtgatggag tgctgactgg tgatattcgc atggctttgt tgcttgaagg aaatgcccat
     3241 taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa gttaccaggc
     3301 taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta caacaaggtt
     3361 aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag acgatccggt
     3421 agcggtcacg gtactggttc tactggttct ggttcttctg agagtgcgat taagccagac
     3481 atgaagatca acctccgtat ggaaggcaac gtaaacgggc accactttgt gatcgacgga
     3541 gatggtacag gcaagccttt tgagggaaaa cagagtatgg atcttgaagt caaagagggc
     3601 ggacctctgc cttttgcctt tgatatcctg accactgcat tccattacgg caacagggta
     3661 ttcgccgaat atccagacca catacaagac tattttaagc agtcgtttcc taaggggtat
     3721 tcgtgggaac gaagcttgac tttcgaagac gggggcattt gcattgccag aaacgacata
     3781 acaatggaag gggacacttt ctataataaa gttcgatttc acggtgtaaa ctttcccgcc
     3841 aatggtccag ttatgcagaa gaagacgctg aaatgggagc cctccactga gaaaatgtat
     3901 gtgcgtgatg gagtgctgac tggtgatatt cgcatggctt tgttgcttga aggaaatgcc
     3961 cattaccgat gtgacttcag aactacttac aaagctaagg agaagggtgt caagttacca
     4021 ggctaccact ttgtggacca ctgcattgag attttaagcc atgacaaaga ttacaacaag
     4081 gttaagctgt atgagcatgc tgttgctcat tctggattgc ctgacaatgc cagacgataa
     4141 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4201 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4261 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4321 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4381 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     4441 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     4501 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     4561 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     4621 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     4681 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     4741 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     4801 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     4861 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4921 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     4981 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5041 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5101 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5161 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     5221 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     5281 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     5341 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     5401 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     5461 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     5521 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     5581 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     5641 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     5701 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     5761 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     5821 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     5881 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     5941 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6001 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6061 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6121 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6181 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6241 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     6301 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     6361 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     6421 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     6481 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     6541 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
     6601 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
     6661 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6721 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6781 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6841 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6901 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6961 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7021 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7081 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7141 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7201 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7261 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7321 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7381 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7441 tgattctgtg gataaccgta ttaccgccat gcattagtta ttaatagtaa tcaattacgg
     7501 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     7561 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     7621 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     7681 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     7741 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     7801 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     7861 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     7921 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     7981 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
     8041 ctggtttagt gaaccgtcag atccgctagc gccacc
//
