LOCUS       sequence-173194-        6474 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 505 / 569, Emission: 516 / 
            581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6474)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-PLK1-N-16
FEATURES             Location/Qualifiers
     source          1..6474
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1858..2538
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     polyA_signal    2660..2781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2697..2716)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2751..2770
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2788..3243)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2925..2946)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3137..3156
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3270..3374
                     /gene="bla"
                     /label=AmpR promoter
     promoter        3376..3733
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3376..3567
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3399..3419)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3584..3719
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3768..4562
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3768..4562
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3822..3841)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4432..4451
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4750..4769)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4794..4841
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5170..5758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5659..5678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        5933..6236
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6237..6440
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6391..6411
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgagtgctg cagtgactgc agggaagctg gcacgggcac cggccgaccc tgggaaagcc
       61 ggggtccccg gagttgcagc tcccggagct ccggcggcgg ctccaccggc gaaagagatc
      121 ccggaggtcc tagtggaccc acgcagccgg cggcgctatg tgcggggccg ctttttgggc
      181 aagggcggct ttgccaagtg cttcgagatc tcggacgcgg acaccaagga ggtgttcgcg
      241 ggcaagattg tgcctaagtc tctgctgctc aagccgcacc agagggagaa gatgtccatg
      301 gaaatatcca ttcaccgcag cctcgcccac cagcacgtcg taggattcca cggctttttc
      361 gaggacaacg acttcgtgtt cgtggtgttg gagctctgcc gccggaggtc tctcctggag
      421 ctgcacaaga ggaggaaagc cctgactgag cctgaggccc gatactacct acggcaaatt
      481 gtgcttggct gccagtacct gcaccgaaac cgagttattc atcgagacct caagctgggc
      541 aaccttttcc tgaatgaaga tctggaggtg aaaatagggg attttggact ggcaaccaaa
      601 gtcgaatatg acggggagag gaagaagacc ctgtgtggga ctcctaatta catagctccc
      661 gaggtgctga gcaagaaagg gcacagtttc gaggtggatg tgtggtccat tgggtgtatc
      721 atgtatacct tgttagtggg caaaccacct tttgagactt cttgcctaaa agagacctac
      781 ctccggatca agaagaatga atacagtatt cccaagcaca tcaaccccgt ggccgcctcc
      841 ctcatccaga agatgcttca gacagatccc actgcccgcc caaccattaa cgagctgctt
      901 aatgacgagt tctttacttc tggctatatc cctgcccgtc tccccatcac ctgcctgacc
      961 attccaccaa ggttttcgat tgctcccagc agcctggacc ccagcaaccg gaagcccctc
     1021 acagtcctca ataaaggctt ggagaacccc ctgcctgagc gtccccggga aaaagaagaa
     1081 ccagtggttc gagagacagg tgaggtggtc gactgccacc tcagtgacat gctgcagcag
     1141 ctgcacagtg tcaatgcctc caagccctcg gagcgtgggc tggtcaggca agaggaggct
     1201 gaggatcctg cctgcatccc catcttctgg gtcagcaagt gggtggacta ttcggacaag
     1261 tacggccttg ggtatcagct ctgtgataac agcgtggggg tgctcttcaa tgactcaaca
     1321 cgcctcatcc tctacaatga tggtgacagc ctgcagtaca tagagcgtga cggcactgag
     1381 tcctacctca ccgtgagttc ccatcccaac tccttgatga agaagatcac cctccttaaa
     1441 tatttccgca attacatgag cgagcacttg ctgaaggcag gtgccaacat cacgccgcgc
     1501 gaaggtgatg agctcgcccg gctgccctac ctacggacct ggttccgcac ccgcagcgcc
     1561 atcatcctgc acctcagcaa cggcagcgtg cagatcaact tcttccagga tcacaccaag
     1621 ctcatcttgt gcccactgat ggcagccgtg acctacatcg acgagaagcg ggacttccgc
     1681 acataccgcc tgagtctcct ggaggagtac ggctgctgca aggagctggc cagccggctc
     1741 cgctacgccc gcactatggt ggacaagctg ctgagctcac gctcggccag caaccgtctc
     1801 aaggcctccg gtagcggcag cgcatccggc ggaagcggaa gcggaccggt cgccaccatg
     1861 agtgcgatta agccagacat gaagatcaac ctccgtatgg aaggcaacgt aaacgggcac
     1921 cactttgtga tcgacggaga tggtacaggc aagccttttg agggaaaaca gagtatggat
     1981 cttgaagtca aagagggcgg acctctgcct tttgcctttg atatcctgac cactgcattc
     2041 cattacggca acagggtatt cgccgaatat ccagaccaca tacaagacta ttttaagcag
     2101 tcgtttccta aggggtattc gtgggaacga agcttgactt tcgaagacgg gggcatttgc
     2161 attgccagaa acgacataac aatggaaggg gacactttct ataataaagt tcgatttcac
     2221 ggtgtaaact ttcccgccaa tggtccagtt atgcagaaga agacgctgaa atgggagccc
     2281 tccactgaga aaatgtatgt gcgtgatgga gtgctgactg gtgatattcg catggctttg
     2341 ttgcttgaag gaaatgccca ttaccgatgt gacttcagaa ctacttacaa agctaaggag
     2401 aagggtgtca agttaccagg ctaccacttt gtggaccact gcattgagat tttaagccat
     2461 gacaaagatt acaacaaggt taagctgtat gagcatgctg ttgctcattc tggattgcct
     2521 gacaatgcca gacgataagc ggccgcgact ctagatcata atcagccata ccacatttgt
     2581 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2641 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2701 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2761 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     2821 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     2881 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     2941 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3001 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3061 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3121 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3181 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3241 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     3301 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     3361 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     3421 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3481 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3541 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3601 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3661 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3721 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     3781 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     3841 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     3901 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     3961 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4021 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4081 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4141 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     4201 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4261 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     4321 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     4381 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     4441 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     4501 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     4561 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     4621 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     4681 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     4741 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     4801 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     4861 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     4921 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     4981 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     5041 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     5101 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     5161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     5221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     5281 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     5341 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     5401 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     5461 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     5521 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     5581 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     5641 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     5701 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5761 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5821 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt
     5881 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
     5941 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
     6001 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
     6061 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
     6121 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
     6181 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
     6241 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
     6301 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
     6361 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
     6421 aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc cacc
//
