LOCUS       sequence-173195-        4914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 553, Emission: 576.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4914)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdTurboRFP-Golgi-7
FEATURES             Location/Qualifiers
     source          1..4914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             268..960
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     polyA_signal    1106..1227
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1143..1162)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1197..1216
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1234..1689)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1371..1392)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1583..1602
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     promoter        1822..2179
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        1822..2013
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(1845..1865)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2030..2165
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2214..3008
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2214..3008
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2268..2287)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2878..2897
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3196..3215)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3240..3287
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3616..4204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4105..4124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4379..4682
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4683..4886
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4837..4857
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgaggcttc gggagccgct cctgagcggc agcgccgcga tgccaggcgc gtccctacag
       61 cgggcctgcc gcctgctcgt ggccgtctgc gctctgcacc ttggcgtcac cctcgtttac
      121 tacctggctg gccgcgacct gagccgcctg ccccaactgg tcggagtctc cacaccgctg
      181 cagggcggct cgaacagtgc cgccgccatc gggcagtcct ccggggagct ccggaccgga
      241 ggggccaagg atccaccggt cgccaccatg agcgagctga tcaaggagaa catgcacatg
      301 aagctgtaca tggagggcac cgtgaacaac caccacttca agtgcacatc cgagggcgaa
      361 ggcaagccct acgagggcac ccagaccatg aagatcaagg tggtcgaggg cggccctctc
      421 cccttcgcct tcgacatcct ggctaccagc ttcatgtacg gcagcaaagc cttcatcaac
      481 cacacccagg gcatccccga cttctttaag cagtccttcc ctgagggctt cacatgggag
      541 agaatcacca catacgaaga cgggggcgtg ctgaccgcta cccaggacac cagcttccag
      601 aacggctgca tcatctacaa cgtcaagatc aacggggtga acttcccatc caacggccct
      661 gtgatgcaga agaaaacacg cggctgggag gccaacaccg agatgctgta ccccgctgac
      721 ggcggcctga gaggccacag ccagatggcc ctgaagctcg tgggcggggg ctacctgcac
      781 tgctccttca agaccacata cagatccaag aaacccgcta agaacctcaa gatgcccggc
      841 ttccacttcg tggaccacag actggaaaga atcaaggagg ccgacaaaga gacctacgtc
      901 gagcagcacg agatggctgt ggccaagtac tgcgacctcc ctagcaaact ggggcacaga
      961 gatgaatccg gactcagatc ttgagcggcc gcgactctag atcataatca gccataccac
     1021 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     1081 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     1141 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     1201 tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa
     1261 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     1321 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     1381 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     1441 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     1501 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     1561 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     1621 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     1681 agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1801 aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     1861 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     1921 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     1981 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     2041 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     2101 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     2161 aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg
     2221 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
     2281 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
     2341 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg
     2401 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
     2461 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
     2521 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
     2581 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
     2641 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
     2701 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg
     2761 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
     2821 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
     2881 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
     2941 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
     3001 tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc
     3061 acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg
     3121 ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc
     3181 tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac
     3241 ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt
     3301 tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc
     3361 ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc
     3421 agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat
     3481 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3541 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3601 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3661 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3721 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     3781 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3841 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3901 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3961 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4021 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4081 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4141 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4201 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4261 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
     4321 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     4381 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     4441 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     4501 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     4561 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     4621 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     4681 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     4741 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     4801 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     4861 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagc
//
