LOCUS       sequence-173198-        6905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Murine homologues of human Trim5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Lascano J, Uchil PD, Mothes W, Luban J
  TITLE     TRIM5 Retroviral Restriction Activity Correlates with the Ability To
            Induce Innate Immune Signaling.
  JOURNAL   J Virol. 2015 Oct 14;90(1):308-16. doi: 10.1128/JVI.02496-15.
  PUBMED    26468522
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-10-14- 14"; volume: "90"; issue:
            "1"; pages: "308-16"
COMMENT     Sequence Label: pcDNA 3.1(-) TRIM30D
FEATURES             Location/Qualifiers
     source          1..6905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      135..270
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     197..216
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             351..1145
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(405..424)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1015..1034
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1319..1440
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1356..1375)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1410..1429
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1489..1505)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1489..1505)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1502..1524)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1513..1529
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1537..1543,1544..1561,1562..1567))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1582..1603
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1720..1737)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1891..2479)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1971..1990)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2650..3441,3442..3510))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3273..3292
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3511..3615)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3690..3709)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3881..4260
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4261..4464
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4415..4435
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4509..4528
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4509..4527
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4547..4556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(6142..6159)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6148..6372
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6418..6846
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6505..6524)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6715..6736
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6855..6875)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(6860..6905,1..284)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
       61 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
      121 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
      181 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
      241 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
      301 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
      361 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
      421 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
      481 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
      541 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
      601 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
      661 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
      721 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
      781 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
      841 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
      901 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
      961 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     1021 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     1081 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     1141 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     1201 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     1261 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     1321 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     1381 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     1441 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     1501 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1561 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1621 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1681 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1741 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1801 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1861 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1921 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1981 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     2041 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     2101 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2161 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2221 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2281 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2341 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2401 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2461 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2521 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2581 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2641 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2701 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2761 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2821 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2881 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2941 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     3001 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     3061 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3121 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3181 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3241 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3301 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3361 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3421 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3481 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3541 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3601 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     3661 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     3721 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     3781 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     3841 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt
     3901 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     3961 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4021 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4081 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4141 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4201 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4261 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4321 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     4381 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     4441 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
     4501 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgcca ccatggcctc
     4561 atcagtcctg gagatgataa aggaagaagt gacctgccca atctgtctgg agctcttgaa
     4621 ggaatctgtg agtgctgact gtaaccacag cttctgcaga gcctgcatca cactgcacta
     4681 tgagtctaac agaaacccag aaggggaggg caactgccct gtgtgccgag ttccttacct
     4741 gtttgagaat ctgaggccta atcgacatgt tgccaacata gtagagaggc tcaagggatt
     4801 caagtccatc ccagaggagg agcagaaggt taatgcctgt gtagaacatg gagagaaact
     4861 ccagctcttc tgtaaggagg acatgatggc catctgctgg ctttgtaagc aatcccagga
     4921 gcacagtggt caccaaacag ctctcattga agaggtcaac catgaataca aggagaagct
     4981 acaggcagct ctgcagaagc tgatggaaaa tgagaaaaga tgtgatgaat ggcaggatga
     5041 ccttcagcaa cagagagctg actgggagaa ccaaatacac agtgatatag aagacattca
     5101 gatagagttt aatggactaa gagacctcct agactccaag gagaatgaga agctgcagga
     5161 gctgaagaaa gagaaggaag aggttatgga aaagctggaa gagtctgaac atgagctaag
     5221 ggagcagagg gagttggtga gagacctcat ctcatatgtt cagcatcagt tggagctgtc
     5281 agtcatggaa atgaagcagg gtgtaaatta tgtcctgaca agcattcaga ccatgacact
     5341 gaagcagccc caaatagttc ccccaaaaag aagaagagga acatccaaag ctccacattt
     5401 gaaaggcatg ctacagtcat ttgaagggct catggatgtt cagcaatact gggttcatat
     5461 gactctacat gcaaggaaca atgcagtcat tgccattaac aaagaaaaaa gacaaataca
     5521 gtatagaagt tacaatacgg ttccagtttc tgagatctac catttgggtg tcctgggata
     5581 tccagctctt tcctcaggga agcattactg ggaagtagac atatctagaa gtgatgcctg
     5641 gctcctcgga ttaaatgacg gaaagtgtgc tcaaccccaa cttcactcaa aggaagaaat
     5701 gggcatcaaa aaaaaatatc attctcatat taaacaaaat gtaacgtttc agcctaaatg
     5761 tggctactgg gttataggga tgaagaattc atctgtatac aatgcctttg atgagtgttc
     5821 tatcacccac aattccagtg tcttggccct ctctctgcct gatcgtccca gtcgtgtagg
     5881 agttttcctg gaccaggaag tttgcactct ctcattttat gatgtttcta actctggagc
     5941 tctcatctat agattctatg acccttcctt ccctgttgaa gtctatccat attttaatcc
     6001 tatggaatgt tcagagccaa tgacagtatg cggaccacca tcctaagcgg ccgccactgt
     6061 gctggatatc tgcagaattc caccacactg gactagtgga tccgagctcg gtaccaagct
     6121 taagtttaaa ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     6181 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     6241 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     6301 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     6361 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     6421 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6481 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6541 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6601 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6661 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6721 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6781 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6841 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6901 aggca
//
