LOCUS       sequence-173202-        4465 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses p9-FLAG-FMO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Myhrvold C, Polka JK, Silver PA
  TITLE     Synthetic Lipid-Containing Scaffolds Enhance Production by 
            Colocalizing Enzymes.
  JOURNAL   ACS Synth Biol. 2016 Aug 3.
  PUBMED    27487319
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Aug 3."
COMMENT     Sequence Label: pCAM105
FEATURES             Location/Qualifiers
     source          1..4465
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    4..22
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    29..47
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             81..103
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             380..403
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(1952..2743)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(2744..2835)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2883..3621)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(3661..4284)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     4192..4211
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(4320..4336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4320..4336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(join(4355..4361,4362..4379,4380..4385))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4400..4421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aattccctat cagtgataga gattgacatc cctatcagtg atagagatac tgagcacgga
       61 tctgcccctc tagaaataat tttgtttaac tttaagaagg agatatacca tgccatttcc
      121 tctggtaaag caagacccaa cctcgaaggc tttcactgaa gccagtgaac gctccaccgg
      181 cacccagatc ctggacgtcg tcaaggcccc tatcggcctg ttcggcgacg atgccaaaca
      241 cgagttcgtg acccgtcagg aacaagccgt ctccgtcgtc agctgggcag ttgctgccgg
      301 tctgatcggc gagctgatcg gctaccgtgg tgcgcgttcg ggtcgcaaag cgatcctggc
      361 caacatccct tttctggccg attataaaga tgatgatgat aaaatggcca ctaggattgc
      421 tatcttaggt gctggtcctt ctggaatggc tcaattgaga gcatttcagt ctgcccagga
      481 gaagggtgcc gaaattcctg agttagtctg cttcgaaaag caggccgatt ggggtggtca
      541 atggaattac acatggagga ccggattgga cgaaaatggt gaacctgtac attcatctat
      601 gtatagatat ttgtggtcta acggacctaa agagtgcttg gagtttgccg attatacctt
      661 tgatgaacat tttggaaaac caatcgcatc ttacccacca agagaagtat tatgggacta
      721 cattaagggt agagttgaga aagctggtgt taggaaatat atcagattta ataccgccgt
      781 tagacatgtc gaatttaacg aggactcaca aaccttcacc gttactgtcc aggaccatac
      841 cactgacact atatattcag aagagtttga ttacgttgtt tgttgcactg gtcatttctc
      901 taccccatac gttccagaat ttgagggatt tgagaagttc ggtggaagaa tattgcatgc
      961 ccacgatttt agagatgcct tagagtttaa agacaagacc gttttattag taggatcttc
     1021 atactcagct gaagacatcg gttctcaatg ttacaaatac ggtgccaaga agttaatttc
     1081 ttgttataga acagcaccaa tgggatataa atggcctgag aactgggatg aaagaccaaa
     1141 tttggttagg gtcgacacag aaaatgccta ctttgctgat ggttcttctg agaaagtaga
     1201 tgctattata ttatgtacag gttacattca tcacttccca ttcttaaatg atgacttgag
     1261 gttagttact aacaacaggt tatggccatt aaatttatat aagggagtag tatgggagga
     1321 taaccctaag ttcttctaca ttggaatgca ggatcagtgg tattcattca atatgtttga
     1381 cgcacaggct tggtatgcta gggatgtaat catgggtaga ttacctttgc catctaagga
     1441 ggaaatgaaa gctgattcaa tggcttggag agaaaaggag ttaacattgg ttaccgctga
     1501 ggagatgtat acataccagg gtgactatat tcagaatttg atcgacatga ctgattatcc
     1561 atctttcgat atccctgcca ccaataaaac ctttcttgag tggaaacatc ataagaaaga
     1621 aaacattatg acattcaggg accattcata taggtcatta atgacaggta ctatggcacc
     1681 taaacaccat actccatgga ttgacgcctt ggatgattca ttagaagctt acttatctga
     1741 caagtcagaa atccctgttg ccaaggaggc tggatcctga atgagtgaga attccatatg
     1801 ctgcagagaa gcttgcggcc gcctcgaggg tacctcgacc ggtaaaccag caatagacat
     1861 aagcggctat ttaacgaccc tgccctgaac cgacgaccgg gtcatcgtgg ccggatcttg
     1921 cggcccctcg gcttgaacga attgttagac attatttgcc gactaccttg gtgatctcgc
     1981 ctttcacgta gtggacaaat tcttccaact gatctgcgcg cgaggccaag cgatcttctt
     2041 cttgtccaag ataagcctgt ctagcttcaa gtatgacggg ctgatactgg gccggcaggc
     2101 gctccattgc ccagtcggca gcgacatcct tcggcgcgat tttgccggtt actgcgctgt
     2161 accaaatgcg ggacaacgta agcactacat ttcgctcatc gccagcccag tcgggcggcg
     2221 agttccatag cgttaaggtt tcatttagcg cctcaaatag atcctgttca ggaaccggat
     2281 caaagagttc ctccgccgct ggacctacca aggcaacgct atgttctctt gcttttgtca
     2341 gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa gatacctgca agaatgtcat
     2401 tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg ataacgccac ggaatgatgt
     2461 cgtcgtgcac aacaatggtg acttctacag cgcggagaat ctcgctctct ccaggggaag
     2521 ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt tgtttcatca agccttacgg
     2581 tcaccgtaac cagcaaatca atatcactgt gtggcttcag gccgccatcc actgcggagc
     2641 cgtacaaatg tacggccagc aacgtcggtt cgagatggcg ctcgatgacg ccaactacct
     2701 ctgatagttg agtcgatact tcggcgatca ccgcttccct catactcttc ctttttcaat
     2761 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     2821 agaaaaataa acaaatagct agctcactcg gtcgctacgc tccgggcgtg agactgcggc
     2881 gggcgctgcg gacacataca aagttaccca cagattccgt ggataagcag gggactaaca
     2941 tgtgaggcaa aacagcaggg ccgcgccggt ggcgtttttc cataggctcc gccctcctgc
     3001 cagagttcac ataaacagac gcttttccgg tgcatctgtg ggagccgtga ggctcaacca
     3061 tgaatctgac agtacgggcg aaacccgaca ggacttaaag atccccaccg tttccggcgg
     3121 gtcgctccct cttgcgctct cctgttccga ccctgccgtt taccggatac ctgttccgcc
     3181 tttctccctt acgggaagtg tggcgctttc tcatagctca cacactggta tctcggctcg
     3241 gtgtaggtcg ttcgctccaa gctgggctgt aagcaagaac tccccgttca gcccgactgc
     3301 tgcgccttat ccggtaactg ttcacttgag tccaacccgg aaaagcacgg taaaacgcca
     3361 ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt
     3421 tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc
     3481 tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa
     3541 ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga
     3601 tctcaagaag atcctttgat cttttctact gaaccgctct agcaaaaggt tatgctgctt
     3661 ttaagaccca ctttcacatt taagttgttt ttctaatccg caaatgatca attcaaggcc
     3721 gaataagaag gctggctctg caccttggtg atcaaataat tcgatagctt gtcgtaataa
     3781 tggcggcata ctatcagtag taggtgtttc cctttcttct ttagcgactt gatgctcttg
     3841 atcttccaat acgcaaccta aagtaaaatg ccccacagcg ctgagtgcat ataatgcatt
     3901 ctctagtgaa aaaccttgtt ggcataaaaa ggctaattga ttttcgagag tttcatactg
     3961 tttttctgta ggccgtgtac ctaaatgtac ttttgctcca tcgcgatgac ttagtaaagc
     4021 acatctaaaa cttttagcgt tattacgtaa aaaatcttgc cagctttccc cttctaaagg
     4081 gcaaaagtga gtatggtgcc tatctaacat ctcaatggct aaggcgtcga gcaaagcccg
     4141 cttatttttt acatgccaat acaatgtagg ctgctctaca cctagcttct gggcgagttt
     4201 acgggttgtt aaaccttcga ttccgacctc attaagcagc tctaatgcgc tgttaatcac
     4261 tttactttta tctaatctgg acattcgggg tctctccctg gtagcttggc gtaatcatgg
     4321 tcatagctgt ttcctgtgtg agttaacttc cacacaacat acgagccgga agcataaagt
     4381 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     4441 ccgctttcct cgtcaagacg atgcc
//
