LOCUS       sequence-173203-        6138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses p12 and p9-6His-tnaA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6138)
  AUTHORS   Myhrvold C, Polka JK, Silver PA
  TITLE     Synthetic Lipid-Containing Scaffolds Enhance Production by 
            Colocalizing Enzymes.
  JOURNAL   ACS Synth Biol. 2016 Aug 3.
  PUBMED    27487319
REFERENCE   2  (bases 1 to 6138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Aug 3."
COMMENT     Sequence Label: pCAM110
FEATURES             Location/Qualifiers
     source          1..6138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..300
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     223..242
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             311..322
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1183..1200
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2631..2702
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2718..2745
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2786..2815
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2831..2848
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2904..2921)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3074..3120
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3162..3179)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3333..3921)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3413..3432)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4092..4883,4884..4952))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4715..4734
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4953..5057)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5125..5143
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(5147..5190)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             complement(5249..6127)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
       61 tcttttactg gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt
      121 aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
      181 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
      241 ccataagatt agcggatctt acctgacgct ttttatcgca actctctact gtttctccat
      301 acccgctagc aaagaggaga aaagaatggt tatcggtctc ctgaagtatc tcacgcctgc
      361 cgttaaggtg cagatggctg ctcgcgcgtt gggcctgtcc cccgccgaag tcgctgcaat
      421 tgacggcacg ttgggtcgtg tctctgcgat gccagcggtc gcggtcgtgc tgggagggaa
      481 acctctctct ctggccacga tcgcgtcagt tgtgtctgat gcaaacccca gtgccactgt
      541 tggcgcgctt atgcctgctg tacagggcat ggtgagttcc gacgaaggcg cgagtgcgtt
      601 ggctaagacc gtggtaggct tcatggagtc cgaccccaac agcgatgtcc tggttcaact
      661 gctccacaag gtgtcaaact tgccgattgt cggctttggt gacacgcagt atgcagaccc
      721 agctgacttc ttggccaagg gagttttccc tctgatcagg aagccagaag tagaggttca
      781 agctgcgcct ttcacctgtc gtcagtgtga tcatgttgat cacatcactg atgtacctca
      841 aacttcgacc tttgttcaca aatgcacttc gtgcggcttt gtgcagatgg tccaccgtaa
      901 ggatgttccg taatgccatt tcctcttgta aagcaagacc caacctcgaa ggctttcact
      961 gaagccagtg aacgctccac cggcacccag atcctggacg tcgtcaaggc ccctatcggc
     1021 ctgttcggcg acgatgccaa acacgagttc gtgacccgtc aggaacaagc cgtctccgtc
     1081 gtcagctggg cagttgctgc cggtctgatc ggcgagctga tcggctaccg tggtgcgcgt
     1141 tcgggtcgca aagcgatcct ggccaacatc cctttcctgg cccaccacca ccaccaccat
     1201 gaaaacttta aacatctccc tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt
     1261 accactcgcg cttatcgtga agaggcaatt attaaatccg gtatgaaccc gttcctgctg
     1321 gatagcgaag atgtttttat cgatttactg accgacagcg gcaccggggc ggtgacgcag
     1381 agcatgcagg ctgcgatgat gcgcggcgac gaagcctaca gcggcagtcg tagctactat
     1441 gcgttagccg agtcagtgaa aaatatcttt ggttatcaat acaccattcc gactcaccag
     1501 ggccgtggcg cagagcaaat ctatattccg gtactgatta aaaaacgcga gcaggaaaaa
     1561 ggcctggatc gcagcaaaat ggtggcgttc tctaactatt tctttgatac cacgcagggc
     1621 catagccaga tcaacggctg taccgtgcgt aacgtctata tcaaagaagc cttcgatacg
     1681 ggcgtgcgtt acgactttaa aggcaacttt gaccttgagg gattagaacg cggtattgaa
     1741 gaagttggtc cgaataacgt gccgtatatc gttgcaacca tcaccagtaa ctctgcaggt
     1801 ggtcagccgg tttcactggc aaacttaaaa gcgatgtaca gcatcgcgaa gaaatacgat
     1861 attccggtgg taatggactc cgcgcgcttt gctgaaaacg cctatttcat caagcagcgt
     1921 gaagcagaat acaaagactg gaccatcgag cagatcaccc gcgaaaccta caaatatgcc
     1981 gatatgctgg cgatgtccgc caagaaagat gcgatggtgc cgatgggcgg cctgctgtgc
     2041 atgaaagacg acagcttctt tgatgtgtac accgagtgca gaaccctttg cgtggtgcag
     2101 gaaggcttcc cgacatatgg cggcctggaa ggcggcgcga tggagcgtct ggcggtaggt
     2161 ctgtatgacg gcatgaatct cgactggctg gcttatcgta tcgcgcaggt acagtatctg
     2221 gtcgatggtc tggaagagat tggcgttgtc tgccagcagg cgggcggtca cgcggcattc
     2281 gttgatgccg gtaaactgtt gccgcatatc ccggcagacc agttcccggc acaggcgctg
     2341 gcctgcgagc tgtataaagt cgccggtatc cgtgcggtag aaattggctc tttcctgtta
     2401 ggccgcgatc cgaaaaccgg taaacaactg ccatgcccgg ctgaactgct gcgtttaacc
     2461 attccgcgcg caacatatac tcaaacacat atggacttca ttattgaagc ctttaaacat
     2521 gtgaaagaga acgcggcgaa tattaaagga ttaaccttta cgtacgaacc gaaagtattg
     2581 cgtcacttca ccgcaaaact taaagaagtt taatctggat ctccaggcat caaataaaac
     2641 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     2701 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat aggatcctaa
     2761 ctcgagtaat gagaagcttg ggcccgaaca aaaactcatc tcagaagagg atctgaatag
     2821 cgccgtcgac catcatcatc atcatcattg agtttaaacg gtctccagct tggctgtttt
     2881 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
     2941 ataaaacaga atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac
     3001 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
     3061 aactgccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     3121 ctgttgtttg tcggtgaact aatttctaga ctgcaggctt cctcgctcac tgactcgctg
     3181 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3241 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3301 aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc ccctgacgag
     3361 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3421 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3481 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     3541 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     3601 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     3661 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     3721 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     3781 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     3841 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     3901 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     3961 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4021 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4081 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4141 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4201 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4261 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4321 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4381 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4441 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4501 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     4561 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     4621 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     4681 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     4741 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     4801 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     4861 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     4921 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     4981 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5041 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     5101 attatcatga cattaaccta taaaaatagg cgtatcacga ggcagaattt cagataaaaa
     5161 aaatccttag ctttcgctaa ggatgatttc tggaattcac tagtctatgc tactccatcg
     5221 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
     5281 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
     5341 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
     5401 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
     5461 cgctaatccc taactgctgg cggaaaagac gtgacagacg cgacggcgac aagcaaacat
     5521 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
     5581 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
     5641 gccgcagcaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
     5701 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
     5761 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
     5821 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
     5881 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
     5941 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
     6001 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
     6061 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
     6121 cagccatact tttcatac
//
