LOCUS       sequence-173205-        6905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Murine homologues of human Trim5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Lascano J, Uchil PD, Mothes W, Luban J
  TITLE     TRIM5 Retroviral Restriction Activity Correlates with the Ability To
            Induce Innate Immune Signaling.
  JOURNAL   J Virol. 2015 Oct 14;90(1):308-16. doi: 10.1128/JVI.02496-15.
  PUBMED    26468522
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-10-14- 14"; volume: "90"; issue:
            "1"; pages: "308-16"
COMMENT     Sequence Label: pcDNA 3.1(-) TRIM12B
FEATURES             Location/Qualifiers
     source          1..6905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..56
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     290..307
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    424..445
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(460..465,466..483,484..490)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    498..514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     503..525
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     522..538
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     522..538
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    587..708
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(598..617)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     652..671
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(882..1676)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(993..1012)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1603..1622
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1743..2072)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1757..1892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1811..1830)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2057..2077
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2086..2514)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2196..2217)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2408..2427
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2560..2784
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2773..2790
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     regulatory      4376..4385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4404..4423)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4405..4423)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4468..4671)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4497..4517)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4672..5051
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5223..5242
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5317..5421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5422..5490,5491..6282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5640..5659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6453..6905,1..136)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
       61 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
      121 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
      181 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
      241 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      301 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
      361 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
      421 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
      481 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
      541 tgattacgcc aagctctagc tagaggtcga cggtatacag acatgataag atacattgat
      601 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      661 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttgg ggtgggcgaa
      721 gaactccagc atgagatccc cgcgctggag gatcatccag ccggcgtccc ggaaaacgat
      781 tccgaagccc aacctttcat agaaggcggc ggtggaatcg aaatctcgtg atggcaggtt
      841 gggcgtcgct tggtcggtca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga
      901 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
      961 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
     1021 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
     1081 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
     1141 ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
     1201 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
     1261 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
     1321 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
     1381 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
     1441 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt
     1501 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
     1561 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
     1621 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatgcga
     1681 aacgatcctc atcctgtctc ttgatcagat ccgaaaatgg atatacaagc tcccgggagc
     1741 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     1801 gcagaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     1861 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
     1921 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     1981 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     2041 cctggggact ttccacaccc taactgacac acattccaca gaattaattc gcgttaaatt
     2101 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     2161 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2221 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2281 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2341 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2401 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2461 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat
     2521 accccctaga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc
     2581 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
     2641 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
     2701 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
     2761 atggctggca actagaaggc acagtcgagg ctgatcagcg gtttaaactt aagcttggta
     2821 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg
     2881 gccgcttaag agtctggcca gcaaattgtc atcggctctg aacttcccgt gggattaaaa
     2941 tatggaaaaa ccctatcagg aaaggaagct gtgcagaatc tgtagataag agtcccattg
     3001 ttggaaatat tgtaaaatga gagagtccca gctgcatagt caaggaaaac accaacacga
     3061 cagggcggga ccatcagaga cagggtcaag acactgggct tgcctgtgaa aggacactcc
     3121 tcaaaagcat tatacacaga ctttttccac agccctataa cccagaagcc atattttggt
     3181 tgataatgtg aatcattctt aatcccccta caaaataaac gatttctttc agcatttgaa
     3241 tgaaatattg gacagaagag gtagcttcca tcacttaacc ccagaaccca agcacctttt
     3301 ccagacacat ctacttccca gtaatgtttt ccagattgga tagctggatg tcccaggacg
     3361 ccttcatgag agttttcaca ctcgtgtata agattacttg cttggtggtc ttcatatcgt
     3421 acttgtcttt tgtcgtcagt aatggcaatg tttggatggt tattttgaac cagggtcacc
     3481 tgaacccagt agcgttgagc ctctgtgacc tcttgcagca cttgcagcat gccttgcagg
     3541 tcaggggctc ggaactttct ttgttccctg gcgatggttt tgggcttctt catcgaaaaa
     3601 gtaagactcc gttttatgat gttctccaca ccctgtagta tttctatgtc tgagcactgt
     3661 aactgatgtt ccacatctga gatgaagtct tctagcaact tgctctgctg agcatgctca
     3721 ctttcagact ctgccaggtt gttgagaatg tcttccttct cttgcctcag cttctgcaat
     3781 tcattcttct cctctgagtc tatgatatcc ctcattcgtt taaactctga ctgaacattc
     3841 tccacatctt tctgtatttg attctcccag taagttctat ccttctgaag ttcatctttc
     3901 cagttttcaa attctttctt atctgccatc agcctttgca gaactacctg cagctgctcc
     3961 ttgtactccc gggccacctc ttcaatgaga gctgttttgt ggccccggtg ctcctgagat
     4021 cgctcacaaa gccagcagat ggccatcatg tccttcctac agaagagctg gagtttctct
     4081 ccatgtcttg cacagttaaa cactttctgc tcctcttctg ggctgggctt gaactctttg
     4141 agcctctcta ctatgttggc cacatgtaga ttaggcctca gattgctaga ctggtaacta
     4201 agtcggcaca cagggcaact gaacatttcc ttatcacatt tgatggattc aaagtacaac
     4261 gtgatgcagc cttggcagaa ggtgtgacca caatctgcac tcacaggttt caccatcagg
     4321 ttcagacaga caggacaggt cacctcctcc tttaaattct tcatgaattg tgaagccatg
     4381 gtggcgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga taagccagta
     4441 agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc accgtacacg
     4501 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
     4561 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     4621 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     4681 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     4741 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     4801 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     4861 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     4921 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
     4981 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagtcaa
     5041 taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac
     5101 cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt
     5161 gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta
     5221 tgcggcatca gagcagattg tactgagagt gcaccatagg ggatcgggag atctcccgat
     5281 ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5341 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5401 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5461 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5521 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5581 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     5641 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     5701 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5761 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5821 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5881 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5941 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     6001 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     6061 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     6121 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     6181 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     6241 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     6301 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     6361 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6421 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6481 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6541 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     6601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6901 ggcag
//
