LOCUS       sequence-173207-        5551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses p9-6His-tnaA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Myhrvold C, Polka JK, Silver PA
  TITLE     Synthetic Lipid-Containing Scaffolds Enhance Production by 
            Colocalizing Enzymes.
  JOURNAL   ACS Synth Biol. 2016 Aug 3.
  PUBMED    27487319
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Aug 3."
COMMENT     Sequence Label: pCAM111
FEATURES             Location/Qualifiers
     source          1..5551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        19..303
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     226..245
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             599..616
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2047..2118
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2134..2161
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2202..2231
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2247..2264
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2320..2337)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2490..2536
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2578..2595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2749..3337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2829..2848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3508..4299,4300..4368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4131..4150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4369..4473)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4541..4559
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4563..4606)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             complement(4665..5543)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat tgccgtcact
       61 gcgtctttta ctggctcttc tcgctaacca aaccggtaac cccgcttatt aaaagcattc
      121 tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc tataatcacg
      181 gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc atagcatttt
      241 tatccataag attagcggat cttacctgac gctttttatc gcaactctct actgtttctc
      301 catacccgct agctaaggat gttccgtaat gccatttcct cttgtaaagc aagacccaac
      361 ctcgaaggct ttcactgaag ccagtgaacg ctccaccggc acccagatcc tggacgtcgt
      421 caaggcccct atcggcctgt tcggcgacga tgccaaacac gagttcgtga cccgtcagga
      481 acaagccgtc tccgtcgtca gctgggcagt tgctgccggt ctgatcggcg agctgatcgg
      541 ctaccgtggt gcgcgttcgg gtcgcaaagc gatcctggcc aacatccctt tcctggccca
      601 ccaccaccac caccatgaaa actttaaaca tctccctgaa ccgttccgca ttcgtgttat
      661 tgagccagta aaacgtacca ctcgcgctta tcgtgaagag gcaattatta aatccggtat
      721 gaacccgttc ctgctggata gcgaagatgt ttttatcgat ttactgaccg acagcggcac
      781 cggggcggtg acgcagagca tgcaggctgc gatgatgcgc ggcgacgaag cctacagcgg
      841 cagtcgtagc tactatgcgt tagccgagtc agtgaaaaat atctttggtt atcaatacac
      901 cattccgact caccagggcc gtggcgcaga gcaaatctat attccggtac tgattaaaaa
      961 acgcgagcag gaaaaaggcc tggatcgcag caaaatggtg gcgttctcta actatttctt
     1021 tgataccacg cagggccata gccagatcaa cggctgtacc gtgcgtaacg tctatatcaa
     1081 agaagccttc gatacgggcg tgcgttacga ctttaaaggc aactttgacc ttgagggatt
     1141 agaacgcggt attgaagaag ttggtccgaa taacgtgccg tatatcgttg caaccatcac
     1201 cagtaactct gcaggtggtc agccggtttc actggcaaac ttaaaagcga tgtacagcat
     1261 cgcgaagaaa tacgatattc cggtggtaat ggactccgcg cgctttgctg aaaacgccta
     1321 tttcatcaag cagcgtgaag cagaatacaa agactggacc atcgagcaga tcacccgcga
     1381 aacctacaaa tatgccgata tgctggcgat gtccgccaag aaagatgcga tggtgccgat
     1441 gggcggcctg ctgtgcatga aagacgacag cttctttgat gtgtacaccg agtgcagaac
     1501 cctttgcgtg gtgcaggaag gcttcccgac atatggcggc ctggaaggcg gcgcgatgga
     1561 gcgtctggcg gtaggtctgt atgacggcat gaatctcgac tggctggctt atcgtatcgc
     1621 gcaggtacag tatctggtcg atggtctgga agagattggc gttgtctgcc agcaggcggg
     1681 cggtcacgcg gcattcgttg atgccggtaa actgttgccg catatcccgg cagaccagtt
     1741 cccggcacag gcgctggcct gcgagctgta taaagtcgcc ggtatccgtg cggtagaaat
     1801 tggctctttc ctgttaggcc gcgatccgaa aaccggtaaa caactgccat gcccggctga
     1861 actgctgcgt ttaaccattc cgcgcgcaac atatactcaa acacatatgg acttcattat
     1921 tgaagccttt aaacatgtga aagagaacgc ggcgaatatt aaaggattaa cctttacgta
     1981 cgaaccgaaa gtattgcgtc acttcaccgc aaaacttaaa gaagtttaat ctggatctcc
     2041 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2101 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2161 gtttatagga tcctaactcg agtaatgaga agcttgggcc cgaacaaaaa ctcatctcag
     2221 aagaggatct gaatagcgcc gtcgaccatc atcatcatca tcattgagtt taaacggtct
     2281 ccagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga
     2341 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc
     2401 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc
     2461 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact
     2521 gggcctttcg ttttatctgt tgtttgtcgg tgaactaatt tctagactgc aggcttcctc
     2581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccacaggct
     2761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3181 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     3781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     3841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     3901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     3961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4501 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
     4561 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcactagt
     4621 ctatgctact ccatcgagcc gtcaattgtc tgattcgtta ccaattatga caacttgacg
     4681 gctacatcat tcactttttc ttcacaaccg gcacggaact cgctcgggct ggccccggtg
     4741 cattttttaa atacccgcga gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg
     4801 gtggcgatag gcatccgggt ggtgctcaaa agcagcttcg cctggctgat acgttggtcc
     4861 tcgcgccagc ttaagacgct aatccctaac tgctggcgga aaagacgtga cagacgcgac
     4921 ggcgacaagc aaacatgctg tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga
     4981 tcgctgatgt actgacaagc ctcgcgtacc cgattatcca tcggtggatg gagcgactcg
     5041 ttaatcgctt ccatgcgccg cagcaacaat tgctcaagca gatttatcgc cagcagctcc
     5101 gaatagcgcc cttccccttg cccggcgtta atgatttgcc caaacaggtc gctgaaatgc
     5161 ggctggtgcg cttcatccgg gcgaaagaac cccgtattgg caaatattga cggccagtta
     5221 agccattcat gccagtaggc gcgcggacga aagtaaaccc actggtgata ccattcgcga
     5281 gcctccggat gacgaccgta gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc
     5341 ggtcggcaaa caaattctcg tccctgattt ttcaccaccc cctgaccgcg aatggtgaga
     5401 ttgagaatat aacctttcat tcccagcggt cggtcgataa aaaaatcgag ataaccgttg
     5461 gcctcaatcg gcgttaaacc cgccaccaga tgggcattaa acgagtatcc cggcagcagg
     5521 ggatcatttt gcgcttcagc catacttttc a
//
