LOCUS       sequence-173210-        6905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Murine homologues of human Trim5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6905)
  AUTHORS   Lascano J, Uchil PD, Mothes W, Luban J
  TITLE     TRIM5 Retroviral Restriction Activity Correlates with the Ability To
            Induce Innate Immune Signaling.
  JOURNAL   J Virol. 2015 Oct 14;90(1):308-16. doi: 10.1128/JVI.02496-15.
  PUBMED    26468522
REFERENCE   2  (bases 1 to 6905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-10-14- 14"; volume: "90"; issue:
            "1"; pages: "308-16"
COMMENT     Sequence Label: pcDNA 3.1(-) TRIM12C
FEATURES             Location/Qualifiers
     source          1..6905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     274..293
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     527..544
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    661..682
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(697..702,703..720,721..727)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    735..751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     740..762
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     759..775
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     759..775
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    824..945
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(835..854)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     889..908
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1119..1913)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1230..1249)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1840..1859
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1980..2309)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1994..2129
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2048..2067)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2294..2314
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2323..2751)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2433..2454)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2645..2664
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2797..3021
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3010..3027
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     regulatory      4613..4622
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4641..4660)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4642..4660)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4705..4908)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4734..4754)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4909..5288
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5460..5479
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5554..5658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5659..5727,5728..6519)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5877..5896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(6690..6905,1..373)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
       61 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      121 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      181 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
      241 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
      301 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
      361 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
      421 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
      481 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
      541 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
      601 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
      661 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
      721 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      781 ttacgccaag ctctagctag aggtcgacgg tatacagaca tgataagata cattgatgag
      841 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
      901 gctattgctt tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa
      961 ctccagcatg agatccccgc gctggaggat catccagccg gcgtcccgga aaacgattcc
     1021 gaagcccaac ctttcataga aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg
     1081 cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     1141 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     1201 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     1261 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     1321 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     1381 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     1441 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     1501 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     1561 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     1621 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     1681 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca
     1741 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     1801 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     1861 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     1921 gatcctcatc ctgtctcttg atcagatccg aaaatggata tacaagctcc cgggagcttt
     1981 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggca
     2041 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg
     2101 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct
     2161 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
     2221 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct
     2281 ggggactttc cacaccctaa ctgacacaca ttccacagaa ttaattcgcg ttaaattttt
     2341 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     2401 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     2461 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     2521 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     2581 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     2641 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     2701 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tggggatacc
     2761 ccctagagcc ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag
     2821 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     2881 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     2941 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     3001 gctggcaact agaaggcaca gtcgaggctg atcagcggtt taaacttaag cttggtaccg
     3061 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
     3121 gcttaagagt ctggccagca aattgtcatg ggctctgaac ttcccatggg attaaaatat
     3181 ggaaaaaccc tatcaggaaa ggaagctgta cagaatctgt agataagagt cccattgttg
     3241 gaaatattgt aaaatgagag agtcccagct gcatagtcaa ggaaaacacc aacacgacag
     3301 ggcgggacca tcagagacag ggtcaagaca ctgggcttgc ctgtgaaagg acactcctca
     3361 aaagcattat acacagactt tttccacagc cctataaccc agaagccata ttttggttga
     3421 taatgtgaat cattcttaat ccccctgcga aataaagggt ttctttcagc atttgaacga
     3481 aatattggat tgaagaggta gcttccatca cttaacccca gaacccaagc accttttcca
     3541 gacacatcta cttcccagta atgttttcct gattggataa ctgggtgtcc caggacacct
     3601 tcatgagagt tttcacagcc atgttcaaga ttccttgctt ggaggtcttc atatcgtatt
     3661 tgccttttgt catcagtaat ggcaatgttt ggattgttgt tttgaaccag cgtcacctga
     3721 acccagtagc gttgagcctc tgtgacctct tgcagcactt gcagcatgcc ttgcaggtca
     3781 ggggctcgga actttctttg ttccctggcg atggctttgg gcttcttcat cgaaaaagta
     3841 tgactccgtt ttatgatgtt ctccacaccc tgcagtattt ctatgtctga gcactgtaac
     3901 tgatgttcca catctgaaat gaagtcttct agcaacttgc tctgctgagc atgctcattt
     3961 tcagactctg ccagtttgtt gataatgttt tccttctctt gcttcagctt ctgcaattcc
     4021 ttcttctctt cagagttcat gatatccctc attcgtttaa actctgactg aacattctcc
     4081 acatctttct gtatttgatt ctcccagtaa gttctatcct tctgaaggtc atctttccag
     4141 ttttcaaatt ctttcttgtc tgccatcagc ctttgcagaa ctacctgcag ctgctccttg
     4201 tactcctggg ccacctcttc aatgagagct gttttgtggc cccggtgctc ctgagatcgc
     4261 tcacaaagcc agcagatggc catcatgtcc ttcctacaga agagctggag tttctctcca
     4321 tgtcttgcac agttaaacac tttctgctcc tcttctgggc taggcttgaa ctctttgagc
     4381 ctctctacta tgttggccac atgtagatta ggcctcagat tgctagactg gtaactaagt
     4441 cggcacacag ggcaactgaa catttcctta tcgcatttgg tggattcaaa gtacaccgtg
     4501 atgcagcctt ggcagaagct gtgaccacaa tctgcactca caggtttcac catcaggttc
     4561 agacagagag gacaggtcac ttcctccttt aaattcttca tgaattgtga agccatggtg
     4621 gcgctagcca gcttgggtct ccctatagtg agtcgtatta atttcgataa gccagtaagc
     4681 agtgggttct ctagttagcc agagagctct gcttatatag acctcccacc gtacacgcct
     4741 accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt
     4801 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
     4861 gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc
     4921 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     4981 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     5041 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     5101 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     5161 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     5221 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa
     5281 tcaatgtcaa cgcgtatatc tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct
     5341 aagcagattc ttcatgcaat tgtcggtcaa gccttgcctt gttgtagctt aaattttgct
     5401 cgcgcactac tcagcgacct ccaacacaca agcagggagc agatactggc ttaactatgc
     5461 ggcatcagag cagattgtac tgagagtgca ccatagggga tcgggagatc tcccgatccg
     5521 tcgacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5581 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     5641 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     5701 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5761 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5821 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5881 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5941 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     6001 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     6061 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     6121 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     6181 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     6241 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     6301 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     6361 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     6421 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     6481 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     6541 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     6601 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6661 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6721 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6781 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     6841 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6901 gctct
//
