LOCUS       sequence-173212-        5944 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cbbL-mRFP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Myhrvold C, Kotula JW, Hicks WM, Conway NJ, Silver PA
  TITLE     A distributed cell division counter reveals growth dynamics in the 
            gut microbiota.
  JOURNAL   Nat Commun. 2015 Nov 30;6:10039. doi: 10.1038/ncomms10039.
  PUBMED    26615910
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2015-11-30"; volume: "6:10039. doi"; pages: " 
            10.1038/ncomms10039"
COMMENT     Sequence Label: pCAM44
FEATURES             Location/Qualifiers
     source          1..5944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..289
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     212..231
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             300..311
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1734..2408
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     terminator      2426..2497
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2513..2540
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             2581..2610
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2626..2643
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2699..2716)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2869..2915
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2957..2974)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3128..3716)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3208..3227)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3887..4678,4679..4747))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4510..4529
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4748..4852)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4920..4938
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(4942..4985)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     CDS             complement(5044..5922)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSRLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 agagaagaaa ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg
       61 ctcttctcgc taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg
      121 accaaagcca tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac
      181 attgattatt tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta
      241 gcggatctta cctgacgctt tttatcgcaa ctctctactg tttctccata cccgctagca
      301 aagaggagaa aagaatggca gttaaaaagt atagtgctgg tgtaaaagaa taccggcaga
      361 cctattggat gccggaatac acaccgttgg attccgacat ccttgcatgc ttcaaaatca
      421 ccccacaacc gggtgttgat cgcgaagaag ccgcagccgc ggttgcagca gaatcttcaa
      481 ccggcacatg gaccaccgtg tggaccgatt tgctgaccga catggactac tacaaaggcc
      541 gtgcctatcg cattgaagac gtacccggtg acgatgcggc attctatgcc tttatcgcct
      601 acccaatcga cctgttcgaa gaagggtcag ttgttaacgt gtttacctca ctggttggta
      661 acgtgttcgg cttcaaagcg gtacgcggcc tgcgtctgga agatgttcgc ttcccactcg
      721 cctacgttaa aacctgtggc ggcccaccgc acggtattca agtcgaacgc gacaagatga
      781 acaaatatgg tcgcccactg ttgggttgca ccatcaagcc aaaacttggt ttgtctgcga
      841 aaaactacgg ccgtgccgta tacgagtgcc tccgtggcgg cctcgacttc actaaagatg
      901 atgaaaacat caattctcag ccgttcatgc gctggcgcga tcgcttcttg ttcgtacaag
      961 acgcgaccga aactgctgaa gcccaaaccg gcgaacgcaa aggccattac ctcaacgtaa
     1021 cggcgccaac tcctgaagaa atgtacaagc gcgcagaatt cgacaaagaa attggcgcgc
     1081 caatcattat gcacgactac atcaccggtg gcttcacggc caacactggc ttggccaagt
     1141 ggtgtcaaga caacggcgta ctgctgcaca tccaccgtgc gatgcatgcg gttatcgacc
     1201 gtaacccgaa ccacggtatt cacttccgtg ttctgaccaa gattctgcgt ttgtcgggtg
     1261 gcgatcacct gcacaccggt accgttgtcg gcaaactgga aggcgaccgt gcctctactc
     1321 tgggctggat tgatttgctc cgcgaatcgt ttatccctga agatcgctcg cgcggtatct
     1381 tcttcgatca agactggggt tcaatgccag gcgtattcgc tgtggcctct ggtggtattc
     1441 acgtatggca catgcctgcg ctggttaaca tctttggtga cgactctgtc ctccaattcg
     1501 gtggcggtac gctgggtcat ccatggggca acgctgccgg tgctgctgcc aaccgtgttg
     1561 ctctggaagc ctgcgtagaa gcgcgtaacc aaggccgcga tatcgaaaaa gaaggcaaag
     1621 aaattctgac tgctgctgca cagcacagcc cagaactgaa gattgccatg gaaacttgga
     1681 aagagatcaa attcgaattt gacactgtcg acaaactcga cactcaaaat cgtgcgagta
     1741 gcgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
     1801 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
     1861 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
     1921 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
     1981 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
     2041 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
     2101 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
     2161 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
     2221 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
     2281 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
     2341 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
     2401 gtgcttaata aggatctcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     2461 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     2521 ttcgggtggg cctttctgcg tttataggat cctaactcga gtaatgagaa gcttgggccc
     2581 gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca tcatcatcat
     2641 cattgagttt aaacggtctc cagcttggct gttttggcgg atgagagaag attttcagcc
     2701 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
     2761 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
     2821 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
     2881 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaactaattt
     2941 ctagactgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3001 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3061 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3121 tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3181 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3241 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3301 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3361 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3421 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3481 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     3541 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     3601 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     3661 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     3721 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     3781 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     3841 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     3901 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3961 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4021 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4081 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4141 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4201 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4261 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4321 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4381 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4441 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4501 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     4561 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     4621 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     4681 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     4741 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     4801 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     4861 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     4921 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
     4981 atttctggaa ttcactagtc tatgctactc catcgagccg tcaattgtct gattcgttac
     5041 caattatgac aacttgacgg ctacatcatt cactttttct tcacaaccgg cacggaactc
     5101 gctcgggctg gccccggtgc attttttaaa tacccgcgag aaatagagtt gatcgtcaaa
     5161 accaacattg cgaccgacgg tggcgatagg catccgggtg gtgctcaaaa gcagcttcgc
     5221 ctggctgata cgttggtcct cgcgccagct taagacgcta atccctaact gctggcggaa
     5281 aagacgtgac agacgcgacg gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa
     5341 attgctgtct gccaggtgat cgctgatgta ctgacaagcc tcgcgtaccc gattatccat
     5401 cggtggatgg agcgactcgt taatcgcttc catgcgccgc agcaacaatt gctcaagcag
     5461 atttatcgcc agcagctccg aatagcgccc ttccccttgc ccggcgttaa tgatttgccc
     5521 aaacaggtcg ctgaaatgcg gctggtgcgc ttcatccggg cgaaagaacc ccgtattggc
     5581 aaatattgac ggccagttaa gccattcatg ccagtaggcg cgcggacgaa agtaaaccca
     5641 ctggtgatac cattcgcgag cctccggatg acgaccgtag tgatgaatct ctcctggcgg
     5701 gaacagcaaa atatcacccg gtcggcaaac aaattctcgt ccctgatttt tcaccacccc
     5761 ctgaccgcga atggtgagat tgagaatata acctttcatt cccagcggtc ggtcgataaa
     5821 aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc gccaccagat gggcattaaa
     5881 cgagtatccc ggcagcaggg gatcattttg cgcttcagcc atacttttca tactcccgcc
     5941 attc
//
