LOCUS       sequence-173218-        6023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Murine homologues of human Trim5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Lascano J, Uchil PD, Mothes W, Luban J
  TITLE     TRIM5 Retroviral Restriction Activity Correlates with the Ability To
            Induce Innate Immune Signaling.
  JOURNAL   J Virol. 2015 Oct 14;90(1):308-16. doi: 10.1128/JVI.02496-15.
  PUBMED    26468522
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1128/JVI"; 
            journalName: "J Virol"; date: "2015-10-14- 14"; volume: "90"; issue:
            "1"; pages: "308-16"
COMMENT     Sequence Label: pcDNA 3.1(-) TRIM30B
FEATURES             Location/Qualifiers
     source          1..6023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(238..255)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    244..468
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      514..942
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(601..620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     811..832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(951..971)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        956..1285
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1136..1271
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1198..1217
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             1352..2146
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1406..1425)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2016..2035
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    2320..2441
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2357..2376)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2411..2430
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2490..2506)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2490..2506)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2503..2525)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2514..2530
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2538..2544,2545..2562,2563..2568))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2583..2604
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2721..2738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2892..3480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2972..2991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3651..4442,4443..4511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4274..4293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4512..4616)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4691..4710)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4882..5261
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5262..5465
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5416..5436
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5510..5529
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5510..5528
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5548..5557
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atgtgatgaa tggcaggatg accttcagca acagagagct gactgggagg caagtgggac
       61 ccttcttaag ggagtcagcc tgatgcccaa gagggacctg ggcaagaagc tcctggggtc
      121 tttacttcct gggatttggt gagcggccgc cactgtgctg gatatctgca gaattccacc
      181 acactggact agtggatccg agctcggtac caagcttaag tttaaaccgc tgatcagcct
      241 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
      301 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
      361 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
      421 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
      481 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
      541 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      601 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      661 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      721 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      781 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      841 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      901 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
      961 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     1021 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     1081 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     1141 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     1201 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     1261 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     1321 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     1381 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     1441 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     1501 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     1561 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     1621 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     1681 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     1741 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     1801 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     1861 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     1921 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     1981 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     2041 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     2101 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     2161 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     2221 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     2281 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     2341 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2401 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     2461 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2521 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     2581 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     2641 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2701 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3061 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3301 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     3361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3541 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3601 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3721 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3781 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3841 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3961 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4081 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4141 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4261 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4321 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4501 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     4621 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     4681 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     4741 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     4801 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     4861 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     4921 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     4981 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     5041 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     5101 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     5161 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     5221 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
     5281 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
     5341 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
     5401 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
     5461 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
     5521 actataggga gacccaagct ggctagcgcc accatggcct catcaatcct cgagatgata
     5581 aaggacgagg taacctgtcc tatctgtctg gagctcctga aggaacctgt gagcactgat
     5641 tgtgaccaca gcttctgtcg agcctgcatc acactaaact atgagtccag caaaagcaga
     5701 ggcacagaag agggcagctg ccctgtgtgc cgagttcgtg tcccatttgg gaatctgaga
     5761 actaatcgac aaatggccaa tatagttgag aggctcaagg ggttcaagtc cattccagag
     5821 ggggagcaga aagtgaatgt ctgtgcacaa catggagaga aactccagct cttctgtaag
     5881 aaggacatga tggccatctg ctgggtttgt gagagatctc aggagcacca tggtcaccaa
     5941 acagctctca ttgaagaggt ggaccatgag tacaaggaga agctacagac agccctgcag
     6001 aagctgatgg aaaatgagaa aag
//
