LOCUS       sequence-173226-        8393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This lentiviral vector can be used to assay activity of SpCas9-VQR 
            (NGA PAM restricted)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Canver MC, Lessard S, Pinello L, Wu Y, Ilboudo Y, Stern EN, 
            Needleman AJ, Galacteros F, Brugnara C, Kutlar A, McKenzie C, Reid 
            M, Chen DD, Das PP, A Cole M, Zeng J, Kurita R, Nakamura Y, Yuan GC,
            Lettre G, Bauer DE, Orkin SH
  TITLE     Variant-aware saturating mutagenesis using multiple Cas9 nucleases 
            identifies regulatory elements at trait-associated loci.
  JOURNAL   Nat Genet. 2017 Feb 20. doi: 10.1038/ng.3793.
  PUBMED    28218758
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2017 Feb 20. doi: 10.1038/ng.3793."
COMMENT     Sequence Label: lenti-Cas9-VQR-GFP_activity_reporter
FEATURES             Location/Qualifiers
     source          1..8393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(54..73)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     550..570
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             690..755
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(762..764,765..767,768..1484)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKS"
     primer_bind     complement(807..828)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1068..1087)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1415..1436
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1499..2087
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1552..1572)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1970..1981)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2159..2392
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2470..2591
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2507..2526)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2561..2580
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2631..2766
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2786..2805)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2787..2805)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2815..2832)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2815..2831)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2824..2846)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2973..3428
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3060..3079)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3270..3291
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3454..3558
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3559..3627,3628..4419)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3777..3796)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4590..5178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5079..5098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5332..5349
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5466..5487
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5502..5507,5508..5525,5526..5532)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5540..5556
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5545..5567
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5564..5580
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5564..5580
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5599..5619
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5601..5619
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5647..5873
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5874..6054
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6101..6226
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6719..6952
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7137..7181
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7359..7607
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7359..7379
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7530..7549
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7628..7703
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    7759..7876
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(7925..8393,1..42)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8354..8372
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc accatgaccg
       61 agtacaagcc caccgtgcgc ctcgccaccc gcgacgacgt gcccagggcc gtccgcaccc
      121 tcgccgccgc tttcgccgac taccccgcca cccgccacac cgtcgatccc gaccgccaca
      181 tcgagcgggt caccgagctg caagaactct tcctcacccg cgtcgggctc gacatcggca
      241 aggtgtgggt cgctgacgac ggcgccgcag tggctgtctg gaccacaccc gagagcgtcg
      301 aagctggggc ggtgttcgcc gagatcggcc ctcgcatggc cgagttgagc ggttcccggc
      361 tggccgctca gcaacagatg gaaggcctcc tggctccaca ccggcccaag gagcccgcat
      421 ggttcctggc caccgtcggc gtctctcccg accaccaggg caagggtctg ggcagcgccg
      481 tcgtgctccc cggagtggag gctgccgagc gcgccggggt gcccgccttc ctggagacct
      541 ccgcaccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtgg
      601 aggtgcccga aggaccacgc acctggtgca tgacccgcaa gcccggtgcc ctgcagggat
      661 ctggcgaagc cagacacaaa cagaaaattg tgaaacagac tttgaatttt gaccttctca
      721 agttggcggg agacgtggag tccaacccag ggcccgctag catggtgagc aagggcgagg
      781 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      841 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      901 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      961 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     1021 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     1081 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     1141 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     1201 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     1261 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     1321 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1381 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1441 ccgccgggat cactctcggc atggacgagc tgtacaagtc ctaacgcgtt aagtcgacaa
     1501 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     1561 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     1621 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     1681 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     1741 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     1801 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     1861 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     1921 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     1981 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     2041 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga
     2101 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     2161 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     2221 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     2281 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     2341 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat
     2401 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     2461 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     2521 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     2581 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     2641 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2701 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2761 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     2821 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2881 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2941 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     3001 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     3061 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     3121 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     3181 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     3241 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     3301 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     3361 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     3421 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     3481 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     3541 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     3601 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3661 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3721 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3781 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3841 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3901 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3961 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     4021 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     4081 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     4141 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     4201 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     4261 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     4321 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     4381 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     4441 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     4501 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     4561 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     4621 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4681 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4741 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4801 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4861 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4921 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4981 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     5041 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     5101 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     5161 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     5221 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     5281 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     5341 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     5401 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     5461 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     5521 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     5581 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     5641 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     5701 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt
     5761 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     5821 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     5881 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5941 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6001 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     6061 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     6121 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     6181 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     6241 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     6301 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     6361 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     6421 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     6481 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     6541 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     6601 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     6661 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     6721 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     6781 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     6841 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     6901 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     6961 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     7021 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     7081 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     7141 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     7201 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     7261 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     7321 cgtttcagac ccacctccca accccgaggg gacccagaga gggcctattt cccatgattc
     7381 cttcatattt gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg
     7441 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     7501 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     7561 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgtt cgagggcgac
     7621 accctgggtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     7681 aaaaagtggc accgagtcgg tgctttttta agcttggcgt aactagatct tgagacaaat
     7741 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     7801 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     7861 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     7921 cgagggggcc cgggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct
     7981 gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca
     8041 cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc
     8101 ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac
     8161 aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg
     8221 cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg cgcgccgaga
     8281 gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt
     8341 tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg gca
//
