LOCUS       sequence-173229-       10000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  High copy number plasmid 1 to prepare Penn State DNA molecular 
            weight ladders.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Henrici RC, Pecen TJ, Johnston JL, Tan S
  TITLE     The pPSU Plasmids for Generating DNA Molecular Weight Markers.
  JOURNAL   Sci Rep. 2017 May 26;7(1):2438. doi: 10.1038/s41598-017-02693-1.
  PUBMED    28550309
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-05-26"; volume: "7(1):2438. doi"; pages: " 
            10.1038/s41598-017-02693-1"
COMMENT     Sequence Label: pPSU1
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7604..7621)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7604..7620)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7613..7635)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7829..7848)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7948..7970
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8008..8026)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8094..8198
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8199..8267,8268..9059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8417..8436)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9230..9818
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9719..9738
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9972..9989
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttga
      241 tatcatcaaa gccatgaaca aagcagccgc gctggatgaa ctgataccgg ggttgctgag
      301 tgaatatatc gaacagtcag gttaacaggc tgcggcattt tgtccgcgcc gggcttcgct
      361 cactgttcag gccggagcca cagaccgccg ttgaatgggc ggatgctaat tactatctcc
      421 cgaaagaatc cgcataccag gaagggcgct gggaaacact gccctttcag cgggccatca
      481 tgaatgcgat gggcagcgac tacatccgtg aggtgaatgt ggtgaagtct gcccgtgtcg
      541 gttattccaa aatgctgctg ggtgtttatg cctactttat agagcataag cagcgcaaca
      601 cccttatctg gttgccgacg gatggtgatg ccgagaactt tatgaaaacc cacgttgagc
      661 cgactattcg tgatattccg tcgctgctgg cgctggcccc gtggtatggc aaaaagcacc
      721 gggataacac gctcaccaga tatcgaagcc tacgcgctga acgccagcgg tgtggtgaat
      781 atcatcgtgt tcgatccgaa aggctgggcg ctgttccgtt ccttcaaagc cgtcaaggag
      841 aagctggata cccgtcgtgg ctctaattcc gagctggaga cagcggtgaa agacctgggc
      901 aaagcggtgt cctataaggg gatgtatggc gatgtggcca tcgtcgtgta ttccggacag
      961 tacgtggaaa acggcgtcaa aaagaacttc ctgccggaca acacgatggt gctggggaac
     1021 actcaggcac gcggtctgcg cacctatggc tgcattcagg atgcggacgc acagcgcgaa
     1081 ggcattaacg cctctgcccg ttacccgaaa aactgggtga ccaccggcga tccggcgcgt
     1141 gagttcacca tgattcagtc agcaccgctg atgctgctgg ctgaccctga tgagttcgtg
     1201 tccgtacaac tggcgtaatc atggcccttc ggggccattg tttctctgtg gaggagtcca
     1261 tgacgaaaga tgaactgatt gcccgtctcc gctcgctggg tgaacaactg aaccgtgatg
     1321 tcagcctgac ggggacgaaa gaagaactgg cgctccgtgt ggcagagctg aaagaggagc
     1381 ttgatgacac ggatgaaact gccggtcagg acacccctct cagccgggaa aatgtgctga
     1441 ccggacatga aaatgaggtg ggatcagcgc agccggatac cgtgattctg gatacgtctg
     1501 aactggtcac ggtcgtggca ctggtgaagc tgcatactga tgcacttcac gccacgcggg
     1561 atgaacctgt ggcatttgtg ctgccgggaa cggcgtttcg tgtctctgcc ggtgtggcag
     1621 ccgaaatgac agagcgcggc ctggccagaa tgcaataacg ggaggcgctg tggctgattt
     1681 cgataacctg tctgcagctg ccattgcccg cgccgatgaa acgatacgcg ggtacatgga
     1741 tatcggtacc cttcatttcg cataaatcac caactcgttg cccggtaaca acagccagtt
     1801 ccattgcaag tctgagccaa catggtgatg attctgctgc ttgataaatt ttcaggtatt
     1861 cgtcagccgt aagtcttgat ctccttacct ctgattttgc tgcgcgagtg gcagcgacat
     1921 ggtttgttgt tatatggcct tcagctattg cctctcggaa tgcatcgctc agtgttgatc
     1981 tgattaactt ggctgacgcc gccttgccct cgtctatgta tccattgagc attgccgcaa
     2041 tttcttttgt ggtgatgtct tcaagtggag catcaggcag acccctcctt attgctttaa
     2101 ttttgctcat gtaatttatg agtgtcttct gcttgattcc tctgctggcc aggatttttt
     2161 cgtagcgatc aagccatgaa tgtaacgtaa cctgcagatc actgttgatt ctcgctgtca
     2221 gaggcttgtg tttgtgtcct gaaaataact caatgttggc ctgtatagct tcagtgattg
     2281 cgattcgcct gtctctgcct aatccaaact ctttacccgt ccttgggtcc ctgtagcagt
     2341 aatatccatt gtttcttata taaaggttag ggggtaaatc ccggcgctca tgacttcgcc
     2401 ttcttcccat ttctgatcct cttcaaaagg ccacctgtta ctggtcgatt taagtcaacc
     2461 tttaccgctg attcgtggaa cagatactct cttccatcct taaccggagg tgggaatatc
     2521 ctgcattccc gaacccatcg acgaactgtt tcaaggcttc ttggacgtcg ctggcgtgcg
     2581 ttccactcct gaagtgtcaa gtacatcgca aagtctccgc aattacacgc aagaaaaaac
     2641 cgccatcagg cggcttggtg ttctttcagt tcttcaattc gaatattggt tacgtctgca
     2701 tgtgctatct gcgcccatat catccagtgg tcgtagcagt cgttgatgtt ctccgcttcg
     2761 ataactctgt tgaatggctc tccattccat tctcctgtga ctcggaagtg catttatcat
     2821 ctccataaaa caaaacccgc cgtagcgagt tcagataaaa taaatccccg cgagtgcgag
     2881 gattgttatg taatattggg tttaatcatc tatatgtttt gtacagagag ggcaagtatc
     2941 gtttccaccg tactcgtgat aataattttg cacggtatca gtcatttctc gcacattgca
     3001 gaatggggat ttgtcttcat tagacttata aaccttcatg gaatatttgt atgccgactc
     3061 tatatctata ccttcatcta cataaacacc ttcgtgatgt ctgcatggag acaagacacc
     3121 ggatctgcac aacattgata acgcccaatc tttttgctca gactctaact cattgatact
     3181 catttataaa ctccttgcaa tgtatgtcgt ttcagctaaa cggtatcagc aatgtttaga
     3241 tatctctaga cttccgatta gaaacgtcaa ggcagcaatc aggattgcaa tcatggttcc
     3301 tgcatatgat gacaatgtcg ccccaagacc atctctatga gctgaaaaag aaacaccagg
     3361 aatgtagtgg cggaaaagga gatagcaaat gcttacgata acgtaaggaa ttattactat
     3421 gtaaacacca ggcatgattc tgttccgcat aattactcct gctaattaat ccttaacttt
     3481 gcccacctgc cttttaaaac attccagtat atcacttttc attcttgcgt agcaatatgc
     3541 catctcttca gctatctcag cattggtgac cttgttcaga ggcgctgaga gatggccttt
     3601 ttctgataga taatgttctg ttaaaatatc tccggcctca tcttttgccc gcaggctaat
     3661 gtctgaaaat tgaggtgacg ggttaaaaat aatatccttg gcaacctttt ttatatccct
     3721 tttaaatttt ggcttaatga ctatatccaa tgagtcaaaa agctcccctt caatatctgt
     3781 tgcccctaag acctttaata tatcgccaaa tacaggtagc ttggcttcta ccttcaccgt
     3841 tgttcggccg atgaaatgca tatgcataac atcgtctttg gtggttcccc tcatcagtgg
     3901 ctctatctga acgcgctctc cactgcttaa tgacattcct ttcccgatta aaaaatctgt
     3961 cagatcggat gtggtcggcc cgaaaacagt tctggcaaaa ccaatggtgt cgccttcaac
     4021 aaacaaaaaa gatgggaatc ccaatgattc gtcatctgcg aggctgttct taatatcttc
     4081 aactgaagct ttagagcgat ttatcttctg aaccagactc ttgtcatttg ttttggtaaa
     4141 gagaaaagtt tttccatcga tactgatgta tcatgtccaa agaacgatta tctgcagatc
     4201 gaacaacgag aatcaagatc actgagctaa atccccacct gatgtgtgtg ctttgtggag
     4261 ggtacttcat tgatgccaca accataatag aatgtctaca ttccttctgt aaaacgtgta
     4321 ttgttcgtta cctggagacc agcaagtatt gtcctatttg tgatgtccaa gttcacaaga
     4381 ccagaccact actgaatata aggtcagata aaactctcca agatattgta tacaaattag
     4441 ttccagggct tttcaaaaat gaaatgaaga gaagaaggga tttttatgca gctcatcctt
     4501 ctgctgatgc tgccaatggc tctaatgaag atagaggaga ggttgcagat gaagataaga
     4561 gaattataac tgatgatgag ataataagct tatccattga attctttgac cagaacagat
     4621 tggatcggaa agtaaacaaa gacaaagaga aatctaagga ggaggtgaat gataaaagat
     4681 acttacgatg cccagcagca atgactgtga tgcacttaag aaagtttctc agaagtaaaa
     4741 tggacatacc taatactttc cagattgatg tcatgtatga ggaggaacct ttaaaggatt
     4801 attatacact aatggatatt gcctacattt atacctggag aaggaatggt ccacttccat
     4861 tgaaatacag agttcgacct acttgtaaaa gaatgaagat cagtcaccag agagatggac
     4921 tgacaaatgc tggagaactg gaaagtgact ctgggagtga caaggccaac agcccagcag
     4981 gaggtattcc ctccacctct tcttgtttgc ctagccccag tactccagtg cagtctcctc
     5041 atccacagtt tcctcacatt tccagtacta tgaatggaac cagcaacagc cccagcggta
     5101 accaccaatc ttcttttgcc aatagacctc gaaaatcatc agtaaatggg tcatcagcaa
     5161 cttcttctgg ttaatgtaca taatcagatc tctgcagttg atgcgtggat ggagtcccgg
     5221 atttatccgg tgatgagcga tatcccggca ctgtcagatt tgatcaccag tatggtggcc
     5281 agcggctatg actaccggcg cgacgatgat gcgggcttgt ggagttcagc cgatctgact
     5341 tatgtcatta cctatgaaat gtgaggacgc tatgcctgta ccaaatccta caatgccggt
     5401 gaaaggtgcc gggaccaccc tgtgggttta taaggggagc ggtgaccctt acgcgaatcc
     5461 gctttcagac gttgactggt cgcgtctggc aaaagttaaa gacctgacgc ccggcgaact
     5521 gaccgctgag tcctatgacg acagctatct cgatgatgaa gatgcagact ggactgcgac
     5581 cgggcagggg cagaaatctg ccggagatac cagcttcacg ctggcgtgga tgcccggaga
     5641 gcaggggcag caggcgctgc tggcgtggtt taatgaaggc gatacccgtg cctataaaat
     5701 ccgcttcccg aacggcacgg tcgatgtgtt ccgtggctgg gtcagcagta tcggtaaggc
     5761 ggtgacggcg aaggaagtga tcacccgcac ggtgaaagtc accaatgtgg gacgtccgtc
     5821 gatggcagaa gatcgcagca cggtaacagc ggcaaccggc atgaccgtga cgcctgccag
     5881 cacctggatc tctgcagcca tggagatcat ctgccgggac gtggctcggg gctatgagaa
     5941 cgtgcccatt ccctgtgtca acggtgtgga tggggagccc tgccctgagg attacaagta
     6001 catctcagag aactgcgaga cgtccaccat gaacatcgat cgcaacatca cccactaaca
     6061 gcactgcacg tgtgtggacg actgctctag ctccaactgc ctgtgcggcc agctcagcat
     6121 ccggtgctgg tatgacaagg atgggcgatt gctccaggaa tttaacaaga ttgagcctcc
     6181 gctgattttc gagtgtaacc aggcgtgctc atgctggaga aactgcaaga accgggtcgt
     6241 acagagtggc atcaaggtgc ggctacagct ctaccgaaca gccaagatgg gctggggggt
     6301 ccgcgccctg taaaccatcc cacaggggac cttcatctgc gagtatgtcg gggagctgat
     6361 ctctgatgct gaggctgatg tgagagagga tgattcttac ctcttcgact tagacaacaa
     6421 ggatggagag gtgtactgca tagatgcccg ttactatggc aacatcagcc gcttcatcaa
     6481 ccacctgtgt gaccccaaca tcattcccgt ccgggtcttc atgctgcacc aagacctgcg
     6541 atttccacgc atcgccttct tcagttcccg agacatccgg actggggagg agctagggtt
     6601 tgactatggc gaccgcttct gggacatcaa aagcaaatat ttcacctgcc aatgtggctc
     6661 tgagaagtgc aagcactcag ggcccggatc tctgcaggtg cagagtgatt tgccgtggtg
     6721 gctggtctgc cgggggacga ttcataagtt ccgctgtgtg ccgcatctca ccgggcggcg
     6781 ctttgagcac ggtgtgacgg actgttacac actgttccgg gatgcttatc atctggcggg
     6841 gattgagatg ccggactttc atcgtgagga tgactggtgg cgtaacggcc agaatctcta
     6901 tctggataat ctggaggcga cggggctgta tcaggtgccg ttgtcagcgg cacagccggg
     6961 cgatgtgctg ctgtgctgtt ttggttcatc agtgccgaat cacgccgcaa tttactgcgg
     7021 cgacggcgag ctgctgcacc atattcctga acaactgagc aaacgagaga ggtacaccga
     7081 caaatggcag cgacgcacac actccctctg gcgtcaccgg gcatggcgcg catctgcctt
     7141 tacggggatt tacaacgatt tggtcgccgc atcgaccttc gtgtgaaaac gggggctgaa
     7201 gccatccggg cactggccac acagctcccg gcgtttcgtc agaaactgag cgacggctgg
     7261 tatcaggtac ggattgccgg gcgggacgtc agcacgtccg ggttaacggc gcagttacat
     7321 gagactctgc ctgatggcgc tgtaattcat attgttccca gagtcgccgg ggccaagtca
     7381 ggtggcgtat tccagattgt cctgggggct gccgccattg ccggatcatt ctttaccgcc
     7441 ggagccaccc ttgcagcatg gggggcagcc attggggccg gtggtatgac cggcatcctg
     7501 ttttctctcg gtgccagtat ggtgctcggt ggtgtggcgc agatgctggc accgaaagcc
     7561 agaactcccc gtatacagac aacggataac gctgcaggaa ttcactggcc gtcgttttac
     7621 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     7681 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     7741 gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     7801 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7861 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7921 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7981 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     8041 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     8101 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     8161 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     8221 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     8281 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8341 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8401 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8461 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8521 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8581 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8641 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8701 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8761 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8821 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8881 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8941 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     9001 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     9061 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     9121 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     9181 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     9241 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9301 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9361 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9421 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9481 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9541 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9601 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9661 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9721 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9781 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9841 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9901 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9961 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
