LOCUS       sequence-173236-        8658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of constitutively active STAT5B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8658)
  TITLE     Brooks lab unpublished viral plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMX-STAT5B-CA-Neo
FEATURES             Location/Qualifiers
     source          1..8658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             7..600
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    663..1020
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1085..1501
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1431..1453
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1469..1485
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1511..1885
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1823..1839
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(4470..4490)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4475..4804
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4655..4790
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5161..5955
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5215..5234)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5825..5844
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6148..6617
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6747..6764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6918..7506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6998..7017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7670..8461,8462..8530))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8293..8312
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8531..8635)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
       61 caaggcatgg aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga
      121 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
      181 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      241 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      421 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga ttgactgagt
      481 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
      541 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
      601 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
      661 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt
      721 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
      781 actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga cttcgggggc
      841 cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt tttggactct ttggtgcacc
      901 ccccttagag gagggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
      961 tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg
     1021 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaatatggg
     1081 cccgggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1141 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1201 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1261 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1321 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1381 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1441 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1501 ccccatatgg ccatatgaga tcttatatgg ggcacccccg ccccttgtaa acttccctga
     1561 ccctgacatg acaagagtta ctaacagccc ctctctccaa gctcacttac aggctctcta
     1621 cttagtccag cacgaagtct ggagacctct ggcggcagcc taccaagaac aactggaccg
     1681 accggtggta cctcaccctt accgagtcgg cgacacagtg tgggtccgcc gacaccagac
     1741 taagaaccta gaacctcgct ggaaaggacc ttacacagtc ctgctgacca cccccaccgc
     1801 cctcaaagta gacggcatcg cagcttggat acacgccgcc cacgtgaagg ctgccgaccc
     1861 cgggggtgga ccatcctcta gactgccgga tcccagtgtg gtggtacggg aattcggcac
     1921 gagggagagt cggcggccca agccgtcacc ccgggcgggg accgagcgcg ggcgacgacg
     1981 cgcggaggtc ctgcacgggg agggagcgag cgggcgtgcg agagagcgtg cgggaccgga
     2041 gagaccgctg gctctggtgg ggcagaacga ggttgtgaac catggctatg tggatacagg
     2101 ctcagcagct ccagggcgat gcccttcacc agatgcaggc cttgtacggc cagcatttcc
     2161 ccatcgaggt gcgacattat ttatcacagt ggatcgaaag ccaagcctgg gactcaatag
     2221 atcttgataa tccacaggag aacattaagg ccacccagct cctggagggc ctggtgcagg
     2281 agctgcagaa gaaggcggag caccaggtgg gggaagatgg gtttttgctg aagatcaagc
     2341 tggggcacta tgccacacag ctccagagca cgtacgaccg ctgccccatg gagctggttc
     2401 gctgtatccg gcacattctg tacaacgaac agaggctggt tcgcgaagcc aacaacggca
     2461 gctctccagc tggaagtctt gctgacgcca tgtcccagaa gcaccttcag atcaaccaaa
     2521 cgtttgagga gctgcgcctg atcacacagg acacggagaa cgagctgaag aagctgcagc
     2581 agacccaaga gtacttcatc atccagtacc aggagagcct gcggatccaa gctcagtttg
     2641 cccagctggg acagctgaac ccccaggagc gcatgagcag ggagacggcc ctccagcaga
     2701 agcaagtgtc cctggagacc tggctgcagc gagaggcaca gacactgcag cagtaccgag
     2761 tggagctggc tgagaagcac cagaagaccc tgcagctgct gcggaagcag cagaccatca
     2821 tcctggacga cgagctgatc cagtggaagc ggagacagca gctggccggg aacgggggtc
     2881 cccccgaggg cagcctggac gtgctgcagt cctggtgtga gaagctggcc gagatcatct
     2941 ggcagaatcg gcagcagatc cgcagggctg agcgcctgtg ccagcagctg cccatcccag
     3001 gccccgtgga ggagatgctg gctgaggtca acgccaccat cacggacatc atctcagccc
     3061 tggtcaccag cacgttcatc atcgagaagc agcctcctca ggtcctgaag acccagacca
     3121 agtttgcggc cactgtgcgc ctgctggtgg gggggaagct gaatgtgcac atgaaccccc
     3181 cgcaggtgaa ggcgaccatc atcagcgagc agcaggccaa gtccctgctc aagaatgaga
     3241 acacccgcaa tgattacagc ggcgagatcc tgaacaactg ttgcgtcatg gagtaccacc
     3301 aggccactgg cacgctcagc gcccacttca gaaacatgtc cctgaaacga atcaagagat
     3361 ctgaccgccg tggtgcagag tcagtaacgg aagagaagtt cacgatcctg tttgactcac
     3421 agttcagcgt cggtggaaac gagctggtct ttcaagtcaa gaccttgtcg ctcccggtgg
     3481 tggtgattgt tcacggcagc caggacaaca atgccacagc cactgtcctc tgggacaacg
     3541 cctttgcaga gcctggcagg gtgccatttg ccgtgcctga caaggtgctg tggccgcagc
     3601 tgtgtgaagc gctcaacatg aaattcaagg ctgaagtaca gagcaaccgg ggcttgacca
     3661 aggagaacct cgtgttcctg gcacagaaac tgttcaacat cagcagcaac cacctcgagg
     3721 actacaacag catgtccgtg tcctggtccc agttcaaccg ggagaatttg ccaggacgga
     3781 attacacttt ctggcagtgg tttgatggcg tgatggaagt attgaaaaaa catctcaagc
     3841 ctcactggaa tgatggggct atcctgggtt tcgtgaacaa gcaacaggcc cacgacctgc
     3901 tcatcaacaa gccggacggg accttcctgc tgcgcttcag cgactcggaa atcgggggca
     3961 tcaccattgc ttggaagttt gactctcagg agagaatgtt ttggaatctg atgcctttta
     4021 ccactagaga cttctctatc cggtccctcg ctgaccgcct gggggacctg aattacctca
     4081 tatatgtgtt tcctgatcgg ccaaaggatg aagtatattc taagtactac acaccggtcc
     4141 cctgtgagcc cgcaactgcg aaagcagctg acggatacgt gaagccacag atcaagcagg
     4201 tggtccccga gtttgcaaat gcattcacag atgctgggag tggcgccacc tacatggatc
     4261 aggctccttc cccagtcgtg tgccctcagg ctcactacaa catgtaccca cccaacccgg
     4321 actccgtcct tgataccgat ggggacttcg atctggaaga cacgatggac gtggcgcggc
     4381 gcgtggaaga gctcttaggc cggcccatgg acagtcagtg gatccctcac gcacagtcat
     4441 gagcggccgc cagcacagtg gtcgactgtg gaatgtgtgt cagttagggt gtggaaagtc
     4501 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     4561 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     4621 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     4681 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     4741 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     4801 caaaaagctt cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac
     4861 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
     4921 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     4981 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     5041 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     5101 aggatctgat ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc
     5161 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     5221 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     5281 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5341 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5401 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5461 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5521 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5581 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5641 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5701 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5761 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5821 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5881 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5941 gacgagttct tctgagcggg actctggggt tcgcatcgtc gacgataaaa taaaagattt
     6001 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     6061 cttaagtaac gccattttgc aaggcatgga aaaatacata actgagaata gagaagttca
     6121 gatcaaggtc aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa
     6181 gcagttcctg ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca
     6241 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga
     6301 tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg
     6361 acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt
     6421 cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca
     6481 gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg
     6541 catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg
     6601 tcagcggggg tctttcacat gcagcatgta tcaaaattaa tttggttttt tttcttaagt
     6661 atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6721 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttgcgg
     7621 ccggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     7681 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     7741 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     7801 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     7861 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     7921 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     7981 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8041 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8101 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8161 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     8221 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     8281 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     8341 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     8401 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     8461 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     8521 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     8581 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     8641 ttccccgaaa agtgccac
//
