LOCUS       sequence-173238-        8264 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of STAT5A dominant negative C-terminal 
            deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8264)
  TITLE     Brooks lab unpublished viral plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMX-STAT5A-DN(-351)-Neo
FEATURES             Location/Qualifiers
     source          1..8264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             7..600
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    663..1020
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1085..1501
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1431..1453
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1469..1485
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1511..1885
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1823..1839
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(4076..4096)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4081..4410
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4261..4396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4767..5561
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4821..4840)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5431..5450
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             5754..6223
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6353..6370)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6524..7112)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6604..6623)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7276..8067,8068..8136))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7899..7918
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8137..8241)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
       61 caaggcatgg aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga
      121 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
      181 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      241 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      421 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga ttgactgagt
      481 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg
      541 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt
      601 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag
      661 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt
      721 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga
      781 actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga cttcgggggc
      841 cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt tttggactct ttggtgcacc
      901 ccccttagag gagggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc
      961 tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg
     1021 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaatatggg
     1081 cccgggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1141 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1201 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1261 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1321 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1381 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1441 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1501 ccccatatgg ccatatgaga tcttatatgg ggcacccccg ccccttgtaa acttccctga
     1561 ccctgacatg acaagagtta ctaacagccc ctctctccaa gctcacttac aggctctcta
     1621 cttagtccag cacgaagtct ggagacctct ggcggcagcc taccaagaac aactggaccg
     1681 accggtggta cctcaccctt accgagtcgg cgacacagtg tgggtccgcc gacaccagac
     1741 taagaaccta gaacctcgct ggaaaggacc ttacacagtc ctgctgacca cccccaccgc
     1801 cctcaaagta gacggcatcg cagcttggat acacgccgcc cacgtgaagg ctgccgaccc
     1861 cgggggtgga ccatcctcta gactgccgga tcccagtgtg gtggtacggg aattcggcac
     1921 gagaccgccg ccgccgtcaa gagccgtcag gagccgtcag aagccccggc ctggagcgac
     1981 agccgcaggc gctccgcagc accaggtaaa cagccatggc gggctggatt caggcccagc
     2041 agcttcaggg agatgccctg cgccagatgc aagtgttgta tgggcagcat ttccccatcg
     2101 aggtccggca ctacctggcc cagtggatcg agagccagcc gtgggatgct attgacttgg
     2161 ataatcccca ggaccgaggt caggccaccc aactcctgga gggcctggtg caggagctgc
     2221 agaagaaggc ggagcaccag gtgggggaag atgggttttt gctgaagatc aagctggggc
     2281 actatgccac acagctccag aacacgtatg accgctgtcc catggagctg gttcgctgta
     2341 tccgtcacat tctgtacaac gaacagaggc tggttcgcga agccaacaat tgcagctccc
     2401 ctgctggtgt cctggttgac gccatgtccc agaagcacct tcagatcaac caaaggtttg
     2461 aggagctgcg cctgatcaca caggacacgg agaacgagct gaagaagctg cagcagaccc
     2521 aagagtactt catcatccag taccaggaga gcctgcggat ccaagctcag tttgcccagc
     2581 tgggacagct gaacccccag gagcgcatga gcagggagac ggccctccag cagaagcaag
     2641 tgtccctgga gacctggctg cagcgagagg cacagacact gcagcagtac cgagtggagc
     2701 tggctgagaa gcaccagaag accctgcagc tgctgcggaa gcagcagacc atcatcctgg
     2761 acgacgagct gatccagtgg aagcggagac agcagctggc cgggaacggg ggtccccccg
     2821 agggcagcct ggacgtgctg cagtcctggt gtgagaagct ggccgagatc atctggcaga
     2881 accggcagca gatccgcagg gctgagcacc tgtgccagca gctgcccatc ccaggccccg
     2941 tggaggagat gctggctgag gtcaacgcca ccatcacgga catcatctca gctctggtca
     3001 ccagcacgtt catcatcgag aagcagcctc ctcaggtcct gaagacccag accaagtttg
     3061 cggccaccgt gcgcctgctg gtggggggaa agctgaatgt gcacatgaac cccccgcagg
     3121 tgaaggcgac catcatcagc gagcagcagg ccaagtccct gctcaagaat gagaacaccc
     3181 gcaatgagtg cagcggcgag atcctgaaca actgttgcgt catggagtac caccaggcca
     3241 ctggcacgct cagcgcccac ttcagaaaca tgtcactgaa aagaatcaag cgcgccgaca
     3301 ggcgtggtgc agagtcggtg acggaggaga agttcacagt cctgtttgag tctcagttca
     3361 gcgttggcag caacgagctg gtgttccagg tgaagaccct gtccctccct gtggtcgtta
     3421 tcgtccatgg cagccaggac cacaatgcta ctgccaccgt gctgtgggac aatgcctttg
     3481 ctgagccggg cagggtgcca tttgctgtgc ctgacaaggt gctgtggccg cagctgtgtg
     3541 aagcgctcaa catgaaattc aaggctgaag tacagagcaa ccggggcttg accaaggaga
     3601 acctcgtgtt cctggcacag aaactgttca acatcagcag caaccacctc gaggactaca
     3661 acagcatgtc tgtgtcctgg tcccagttca accgggagaa cttgcccggc tggaactaca
     3721 ccttctggca gtggttcgac ggggtgatgg aggtgctgaa gaagcaccat aagccccatt
     3781 ggaatgatgg ggctatcctg ggtttcgtga acaagcaaca ggcccacgac ctgctcatca
     3841 acaagccgga cgggaccttc ctgctgcgct tcagtgactc ggaaatcggg ggcatcacca
     3901 ttgcttggaa gtttgactct ccggaccgaa acctctggaa tctgaagcca ttcacgacgc
     3961 gagatttctc cattcggtcc ctggccgacc ggctggggga cctgaactac cttatctacg
     4021 tgttcccaga ccgacccaag gacgagtagc ggccgccagc acagtggtcg actgtggaat
     4081 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4141 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4201 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4261 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4321 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     4381 ggcttttttg gaggcctagg cttttgcaaa aagcttcacg ctgccgcaag cactcagggc
     4441 gcaagggctg ctaaaggaag cggaacacgt agaaagccag tccgcagaaa cggtgctgac
     4501 cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc gcaaagagaa
     4561 agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt ttatggacag
     4621 caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag ccctgcaaag
     4681 taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca agatctgatc
     4741 aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc
     4801 cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct
     4861 ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg
     4921 acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca
     4981 cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc
     5041 tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga
     5101 aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc
     5161 cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc
     5221 ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg
     5281 ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct
     5341 gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc
     5401 tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc
     5461 ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc
     5521 agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcgc
     5581 atcgtcgacg ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga
     5641 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa
     5701 tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa cagctgaata
     5761 tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga
     5821 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc
     5881 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca
     5941 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac
     6001 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag
     6061 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt
     6121 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg
     6181 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcacatgcag catgtatcaa
     6241 aattaatttg gttttttttc ttaagtattt acattaaatg gccatagttg cattaatgaa
     6301 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6361 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6421 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6481 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6541 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6601 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6661 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6721 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6781 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6841 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6901 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6961 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     7021 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7081 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7141 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7201 ggatcttcac ctagatcctt ttgcggccgg ccgcaaatca atctaaagta tatatgagta
     7261 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     7321 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     7381 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     7441 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     7501 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     7561 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     7621 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     7681 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     7741 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     7801 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     7861 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     7921 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7981 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     8041 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     8101 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     8161 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     8221 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
