LOCUS       sequence-17324-e         621 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Weissinger EM, Mischak H, Goodnight J, Davidson WF, Mushinski JF
  TITLE     Addition of constitutive c-myc expression to Abelson murine leukemia
            virus changes the phenotype of the cells transformed by the virus 
            from pre-B-cell lymphomas to plasmacytomas.
  JOURNAL   Mol Cell Biol 1993 Apr;13(4):2578-85.
  PUBMED    8455630
REFERENCE   2  (bases 1 to 621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "1993-04"; volume: "13(4)"; pages: "2578-85"
COMMENT     Sequence Label: Mouse c-myc (exon I)
FEATURES             Location/Qualifiers
     source          1..621
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(366..382)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(366..382)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(379..401)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    390..406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(414..420,421..438,439..444))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    459..480
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(597..614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcgctgtagt attccagcga gagacagagg gagtgagcgg acggttggaa gagccgtgtg
       61 tgcagagcca cgctccgggg cgacctaaga aggcagctct ggagtgagag gggctttgcc
      121 tccgagcctg ccgcccactc tccccaaccc tgcgactgac ccaacatcag cggccgcaac
      181 cctcgccgcc gctgggaaac tttgcccatt gcagcgggca gacacttctc actggaactt
      241 acaatctgcg agccaggaca ggactcccca ggctccgggg agggaatttt tgtctatttg
      301 gggacagtgt tctctgcctc tgcccgcgat cagctctcct gaaaagagct cgaattcgta
      361 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
      421 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
      481 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
      541 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
      601 ctcactgact cgctgcgctc g
//
