LOCUS       sequence-173254-        6747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter
            in chick embryos.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Martinez-Morales PL, Diez del Corral R, Olivera-Martinez I, Quiroga 
            AC, Das RM, Barbas JA, Storey KG, Morales AV
  TITLE     FGF and retinoic acid activity gradients control the timing of 
            neural crest cell emigration in the trunk.
  JOURNAL   J Cell Biol. 2011 Aug 8;194(3):489-503. doi: 10.1083/jcb.201011077. 
            Epub 2011 Aug 1.
  PUBMED    21807879
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1083/jcb.201011077"; journalName: "J Cell Biol"; date: 
            "2011-08-8- 8"; volume: "194"; issue: "3"; pages: "489-503"
COMMENT     Sequence Label: pCIG-FGFRdn
FEATURES             Location/Qualifiers
     source          1..6747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     5'UTR           124..166
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    1496..2063
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1513..2065
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1657..1674)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1884..1903
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2092..2112
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2116..2136
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2140..2160
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(2170..2172,2173..2175,2176..2889)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2170..2172,2173..2175,2176..2886)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2215..2236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2476..2495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2823..2844
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2981..3000)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3046..3101
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3100..3119)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3492..3508)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3492..3508)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3505..3527)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3516..3532
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3540..3546,3547..3564,3565..3570))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3585..3606
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3665..3860
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3711..3846
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3866..4000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3903..3922)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3957..3976
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4068..4085)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4239..4827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4319..4338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4998..5789,5790..5858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5621..5640
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5859..5963)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5994..6373
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6700..6722
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
       61 ctcatcattt tggcaaagaa ttgctcgagg tcgatcgacg gtatcgataa gcttgatagc
      121 ttgcttgttc tttttgcaga agctcagaat aaacgctcaa ctttggcaga taccatggtt
      181 acctggaggt gcctcatcct ttgggctgtg ctggtcacag ccacgctgtc tgctgccaga
      241 ccggccccca cgctgcccga ccaagctctg cccaaagcga acatcgaggt ggagtcccac
      301 tcggcgcacc ccggcgatct cctccagctg cgctgccggc tgcgcgatga cgtgcagagc
      361 atcaactggg tgcgtgatgg agtgcagctg cccgagaaca accgcacgcg catcaccggc
      421 gaggaggtag aggtgcggga cgcggtgccc gaggactcgg ggctctatgc ctgcatgact
      481 aacagcccct cggggagcga gaccacctac ttctccgtca acgtctcaga cgcactccct
      541 tctgcagagg atgatgatga tgaagatgat tcctcctcgg aggagaagga ggcggataac
      601 accaagccga gccaggctgt agctccttac tggacctatc ccgagaagat ggagaagaag
      661 ctgcatgccg tccccgctgc caaaacagtg aaattcaagt gcccctcagg tgggacgccc
      721 aaccccacgc tgcgctggct gaagaacggc aaggagttca agcctgacca ccgcatcggg
      781 gggtacaagg tccgctatgc cacctggagc atcatcatgg actcggtggt gccatcagat
      841 aagggcaact acacgtgcat cgtggagaac aaatacggga gcatcaacca cacctaccag
      901 ctggatgtcg tggagcgctc cccgcatcgg cccatcctgc aggcagggct ccccgccaac
      961 aaaacggtgg ccctgggcag caacgtggag tttgtctgca aggtctacag cgacccgcag
     1021 ccccacatcc agtggctgaa acacatcgag gtgaacggca gcaagatcgg ccccgacaac
     1081 ttgccctacg tgcagatcct gaagacggct ggcgttaaca cgacagacaa agagatggaa
     1141 gtccttcact taaggaatgt ctcatttgag gatgctgggg agtatacatg tttggcgggt
     1201 aattctattg ggatctccca tcactctgca tggttgacag ttctcgaagc tactgagcag
     1261 tcaccagcca tgatgacgtc ccccctctac ctggagatca tcatttactg caccggcgcc
     1321 ttcctcatct cctgcatggt ggtgacagtc gtcatctaca agatgaagag caccaccaag
     1381 aagacagact tcaacagcca gctggccgtg cacaagctgg ccaagagcat cccactgcgc
     1441 agacaggtaa gaattatcga attcgatatc gcggccgcta gaatagcata gcaattccgc
     1501 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     1561 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     1621 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     1681 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     1741 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     1801 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     1861 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     1921 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac acatgcttta
     1981 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     2041 tcctttgaaa aacacgatga taatatggcc acaaccatgg tgcacgtgga tccaaaaaag
     2101 aagagaaagg tagatccaaa aaagaagaga aaggtagatc caaaaaagaa gagaaaggta
     2161 cacgtgtcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2221 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2281 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2341 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2401 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2461 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2521 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2581 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2641 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2701 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2761 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2821 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2881 tacaagtaaa gcggccgcaa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg
     2941 ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa
     3001 attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta
     3061 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     3121 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     3181 atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc
     3241 cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta
     3301 tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac
     3361 tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat
     3421 ggagatccct cgacctgcac cgtcgaccag ctggtcgacg gtgcaccgtc gaccagcttg
     3481 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     3541 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     3601 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     3661 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     3721 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     3781 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     3841 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     3901 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3961 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     4021 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4921 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4981 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5041 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5101 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5161 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5221 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5281 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5341 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5401 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5461 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5521 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5581 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5641 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5701 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5761 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5821 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5881 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5941 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
     6001 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     6061 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     6121 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     6181 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     6241 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     6301 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     6361 cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     6421 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     6481 gggggcttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     6541 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     6601 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     6661 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     6721 ccggcggctc tagagcctct gctaacc
//
