LOCUS       sequence-173258-        9020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localizes CRY2 to excitatory synapses.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Sinnen BL, Bowen AB, Forte JS, Hiester BG, Crosby KC, Gibson ES, 
            Dell'Acqua ML, Kennedy MJ
  TITLE     Optogenetic Control of Synaptic Composition and Function.
  JOURNAL   Neuron. 2017 Feb 8;93(3):646-660.e5. doi: 
            10.1016/j.neuron.2016.12.037. Epub 2017 Jan 26.
  PUBMED    28132827
REFERENCE   2  (bases 1 to 9020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2016.12.037"; journalName: "Neuron"; date: 
            "2017-02-8- 8"; volume: "93"; issue: "3"; pages: "646-660.e5"
COMMENT     Sequence Label: CRY2PHR-PSD95FingR-GFP
FEATURES             Location/Qualifiers
     source          1..9020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    24..35
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
     enhancer        39..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        421..696
                     /label=chicken beta-actin promoter
     intron          697..1708
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1632..1654
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1716..1735
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1772..3265
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             3578..3601
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(3626..3628,3629..4336)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     CDS             join(3626..3628,3629..4336)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELY"
     primer_bind     complement(3668..3689)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3929..3948)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4276..4297
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4382..4723
                     /codon_start=1
                     /product="zinc-finger domain from the CCR5-224 (+) 
                     zinc-finger nuclease that recognizes the left half-site 
                     target sequence in the human CCR5 gene (Perez et al., 2008;
                     Gross et al., 2013)"
                     /label=CCR5TC
                     /translation="FQCRICMRNFSDRSNLSRHIRTHTGEKPFACDICGRKFAISSNLN
                     SHTKIHTGSQKPFQCRICMRNFSRSDNLARHIRTHTGEKPFACDICGRKFATSGNLTRH
                     TKIHLRGSQL"
     CDS             4772..4897
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the rat zinc finger protein Kid-1 
                     (Witzgall et al., 1994)"
                     /label=KRAB-A
                     /translation="VSVTFEDVAVLFTRDEWKKLDLSQRSLYREVMLENYSNLASM"
     intron          4946..5078
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     5061..5080
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5087..5675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5140..5160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5558..5569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5702..5719)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5708..5819
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    6111..6165
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6164..6183)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6524..6540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6537..6559)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6548..6564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6572..6578,6579..6596,6597..6602))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6617..6638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6697..6892
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6743..6878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6898..7032
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6935..6954)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6989..7008
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7100..7117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7271..7859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7351..7370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8328..8705)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     8652..8671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8890..8994)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agagtcatcc tcatcgtcga cattgattat tgactagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg
      421 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
      481 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      541 cgcgcgccag gcggggcggg gcgggggcga gggcggggcg gggcgaggcg gagaggtgcg
      601 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      661 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
      721 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      781 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
      841 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
      901 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
      961 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     1021 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc gggggggctg
     1081 cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag ggggtgtggg
     1141 cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct gagcacggcc
     1201 cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg ccgggcgggg
     1261 ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg gagggctcgg
     1321 gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga gccgcagcca
     1381 ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc aaatctgtgc
     1441 ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg cgaagcggtg
     1501 cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc gccgccgtcc
     1561 ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc gggggggacg
     1621 gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct ctgctaacca
     1681 tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc
     1741 tcatcatttt ggcaaagaat tcggtaccac catgaagatg gacaaaaaga ctatagtttg
     1801 gtttagaaga gacctaagga ttgaggataa tcctgcatta gcagcagctg ctcacgaagg
     1861 atctgttttt cctgtcttca tttggtgtcc tgaagaagaa ggacagtttt atcctggaag
     1921 agcttcaaga tggtggatga aacaatcact tgctcactta tctcaatcct tgaaggctct
     1981 tggatctgac ctcactttaa tcaaaaccca caacacgatt tcagcgatct tggattgtat
     2041 ccgcgttacc ggtgctacaa aagtcgtctt taaccacctc tatgatcctg tttcgttagt
     2101 tcgggaccat accgtaaagg agaagctggt ggaacgtggg atctctgtgc aaagctacaa
     2161 tggagatcta ttgtatgaac cgtgggagat atactgcgaa aagggcaaac cttttacgag
     2221 tttcaattct tactggaaga aatgcttaga tatgtcgatt gaatccgtta tgcttcctcc
     2281 tccttggcgg ttgatgccaa taactgcagc ggctgaagcg atttgggcgt gttcgattga
     2341 agaactaggg ctggagaatg aggccgagaa accgagcaat gcgttgttaa ctagagcttg
     2401 gtctccagga tggagcaatg ctgataagtt actaaatgag ttcatcgaga agcagttgat
     2461 agattatgca aagaacagca agaaagttgt tgggaattct acttcactac tttctccgta
     2521 tctccatttc ggggaaataa gcgtcagaca cgttttccag tgtgcccgga tgaaacaaat
     2581 tatatgggca agagataaga acagtgaagg agaagaaagt gcagatcttt ttcttagggg
     2641 aatcggttta agagagtatt ctcggtatat atgtttcaac ttcccgttta ctcacgagca
     2701 atcgttgttg agtcatcttc ggtttttccc ttgggatgct gatgttgata agttcaaggc
     2761 ctggagacaa ggcaggaccg gttatccgtt ggtggatgcc ggaatgagag agctttgggc
     2821 taccggatgg atgcataaca gaataagagt gattgtttca agctttgctg tgaagtttct
     2881 tctccttcca tggaaatggg gaatgaagta tttctgggat acacttttgg atgctgattt
     2941 ggaatgtgac atccttggct ggcagtatat ctctgggagt atccccgatg gccacgagct
     3001 tgatcgcttg gacaatcccg cgttacaagg cgccaaatat gacccagaag gtgagtacat
     3061 aaggcaatgg cttcccgagc ttgcgagatt gccaactgaa tggatccatc atccatggga
     3121 cgctccttta accgtactca aagcttctgg tgtggaactc ggaacaaact atgcgaaacc
     3181 cattgtagac atcgacacag ctcgtgagct actagctaaa gctatttcaa gaacccgtga
     3241 agcacagatc atgatcggag cagcagatag tgctggtagt gctggtagtg ctggtcccgg
     3301 gattacaatg ctcgaagtca aggaagcatc accaaccagc atccagatca gctgggtgct
     3361 ccacttgcgc cacgttcgct actaccgcat cacctacggt gaaactggtg gcaatagccc
     3421 tgtccaggaa ttcaccgtgc ctggcagcaa gtccactgct accatcagcg gcctgaaacc
     3481 tggtgtcgac tataccatca cggtgtacgc cgtcacgatc ttcagcgcct accgctccgc
     3541 ctggccgccg atctccatca actaccgcac cggaaccgat tacaaggatg acgacgataa
     3601 gggtagcggc tccagtagat ctggggtgag caagggcgag gagctgttca ccggggtggt
     3661 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     3721 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3781 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3841 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3901 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3961 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4021 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     4081 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     4141 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     4201 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     4261 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     4321 catggacgag ctgtactcta ggggagctgg cgctggagcg ggtgcagggg ctggctctag
     4381 attccagtgc cggatctgca tgcggaactt cagcgaccgg tccaacctga gcaggcacat
     4441 cagaacccac accggagaaa agcccttcgc ctgcgacatt tgcggccgga agttcgccat
     4501 cagcagcaac ctgaacagcc acaccaagat ccacactggc agccagaaac ctttccagtg
     4561 cagaatttgt atgagaaact ttagcagaag cgacaacctg gccagacaca tccggacaca
     4621 tactggtgaa aaaccttttg cctgtgatat ctgtggcaga aagtttgcca cctccggcaa
     4681 tctgacccgg cacacaaaga ttcacctgcg gggcagccag ctatcgattg tcgacgctcc
     4741 tgaacaacgt gaaggtgctt ctcaagtttc tgtttctgtt acttttgaag atgttgctgt
     4801 tctttttact cgtgatgaat ggaaaaaact tgatctttct caacgttctc tttatcgtga
     4861 agttatgctt gaaaattatt ctaatcttgc ttctatggct taaacgcgtt gtacaagtaa
     4921 agcggccccg gactcgaggc cgcaggtaag tatcaaggtt acaagacagg tttaaggaga
     4981 ccaatagaaa ctgggcttgt cgagacagag aagactcttg cgtttctgat aggcacctat
     5041 tggtcttact gacatccact ttgcctttct ctccacaggt gtcgacaatc aacctctgga
     5101 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5161 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5221 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5281 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5341 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5401 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5461 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     5521 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5581 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5641 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtacgatca
     5701 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5761 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5821 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5881 gaggattggg aagacaatag caggccagct tttgttccct ttagggttaa ttgcgcgctt
     5941 ggcgctaatc atggtcatag ctgtttccac tcctcaggtg caggctgcct atcagaaggt
     6001 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcgtt ttccctctgc
     6061 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     6121 atttattttc attgcaatag tgtgttgaat tttttgtgtc tctcactcgg aaggacatat
     6181 gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat
     6241 gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt atatgaaaca
     6301 gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt
     6361 ttatattttg tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt
     6421 actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt
     6481 atgaagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg
     6541 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6601 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6661 ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat
     6721 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6781 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6841 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac
     6901 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6961 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7021 catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7081 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     7381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     7441 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
     7501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     7561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     7621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     7681 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     7741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     7801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     7861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     7921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     7981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     8341 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     8401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     8461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     8521 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8581 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8641 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8701 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8761 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8821 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8881 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8941 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9001 tccccgaaaa gtgccacctg
//
