LOCUS       sequence-173262-        2639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Adenoviral expression of STAT5A dominant negative with co-expression
            of GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Legrand JM, Roy E, Ellis JJ, Francois M, Brooks AJ, Khosrotehrani K
  TITLE     STAT5 Activation in the Dermal Papilla Is Important for Hair 
            Follicle Growth Phase Induction.
  JOURNAL   J Invest Dermatol. 2016 Sep;136(9):1781-91. doi: 
            10.1016/j.jid.2016.04.014. Epub 2016 Apr 27.
  PUBMED    27131881
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jid.2016.04.014"; journalName: "J Invest Dermatol"; date:
            "2016-09"; volume: "136"; issue: "9"; pages: "1781-91"
COMMENT     Sequence Label: pAD-CMV-STAT5A-DN-CMV-GFP
FEATURES             Location/Qualifiers
     source          1..2639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             199..668
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(798..815)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(969..1557)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1049..1068)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1721..2512,2513..2581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2344..2363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
       61 aaagtgccac ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
      121 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt
      181 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
      241 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg
      301 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca
      361 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
      421 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
      481 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
      541 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
      601 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
      661 gtctttcaca tgcagcatgt atcaaaatta atttggtttt ttttcttaag tatttacatt
      721 aaatggccat agttgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
      781 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      841 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      901 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      961 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1021 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1081 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1141 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1201 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1261 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1321 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1381 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1441 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1501 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1561 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1621 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgcg gccggccgca
     1681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     1741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     2401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     2461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     2521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     2581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcgga
//
