LOCUS       sequence-17327-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Beresford PJ, Zhang D, Oh DY, Fan Z, Greer EL, Russo ML, Jaju M, 
            Lieberman J
  TITLE     Granzyme A activates an endoplasmic reticulum-associated 
            caspase-independent nuclease to induce single-stranded DNA nicks.
  JOURNAL   J Biol Chem 2001 Nov 16;276(46):43285-93. Epub 2001 Sep 12.
  PUBMED    11555662
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem"; date: "2001-11-16"; volume: "276(46)"; pages: "43285-93. Epub
            2001 Sep 12"
COMMENT     Sequence Label: pET30a-PP32
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             64..81
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             88..132
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             148..162
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 aatattttgt ttactttaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtctggtgc cacgcggttc tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag
      121 cacatggaca gcccagatct gggtaccgac gacgacgaca aggccatgga gatgggcaga
      181 cggattcatt tagagctgcg gaacaggacg ccctctgatg tgaaagaact tgtcctggac
      241 aacagtcggt cgaatgaagg caaactcgaa ggcctcacag atgaatttga agaactggaa
      301 ttcttaagta caatcaacgt aggcctcacc tcaatcgcaa acttaccaaa gttaaacaaa
      361 cttaagaagc ttgaactaag cgataacaga gtctcagggg gcctggaagt attggcagaa
      421 aagtgtccga acctcacgca tctaaattta agtggcaaca aaattaaaga cctcagcaca
      481 atagagccac tgaaaaagtt agaaaacctc aagagcttag accttttcaa ttgcgaggta
      541 accaacctga acgactaccg agaaaatgtg ttcaagctcc tcccgcaact cacatatctc
      601 gacggctatg accgggacga caaggaggcc cctgactcgg atgctgaggg ctacgtggag
      661 ggcctggatg atgaggagga ggatgaggat gaggaggagt atgatgaaga tgctcaggta
      721 gtggaagacg aggaggacga ggatgaggag gaggaaggtg aagaggagga cgtgagtgga
      781 gaggaggagg atgaag
//
