LOCUS       sequence-173270-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mCherry-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(55..72)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(182..206)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(203..241)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(274..292,293..315,316..334,335..357,358..376,377..399,
                     400..418,419..441,442..460,461..483,484..502,503..525,
                     526..544)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     primer_bind     complement(601..617)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(633..655)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacag atccgtgatg
       61 gtggtgatgg tgtgccatgg tatatgctcc gaattcactt tacggatctg gggccgcgga
      121 ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg
      181 cgatctgacg gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcctac
      241 ctcgacccgg gtaccgagct cgactttcac ttttctctat cactgatagg gagtggtaaa
      301 ctcgactttc acttttctct atcactgata gggagtggta aactcgactt tcacttttct
      361 ctatcactga tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt
      421 ggtaaactcg actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac
      481 ttttctctat cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata
      541 gggagtggta aactcgacga cgaaagggcc tcgtggatcc ggccggcgcc tagagaagga
      601 gtgagggctg gataaaggga ggatcgaggc ggggtcgaac gaggaggttc aagggggaga
      661 gacggggcgg atggaggaag aggaggcgga ggcttagggt gtacaaaggg cttgacccag
      721 ggaggggg
//
