LOCUS       sequence-173271-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of photoactivatable Rac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn 
            KM
  TITLE     A genetically encoded photoactivatable Rac controls the motility of 
            living cells.
  JOURNAL   Nature. 2009 Sep 3. 461(7260):104-8.
  PUBMED    19693014
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Sep 3. 461(7260):104-8."
COMMENT     Sequence Label: pBabe-TetCMV-puro-mCherry-PA-Rac1
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(4..503)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(52..71)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     445..468
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    536..552
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(566..598)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
ORIGIN
        1 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
       61 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
      121 cctgagcccg cccccggacc caccccttcc cagcctctga gcccagaaag cgaaggagca
      181 aagctgctat tggccgctgc cccaaaggcc tacccgcttc cattgctcag cggtgctgtc
      241 catctgcacg agactagtga gacgtgctac ttccatttgt cacgtcctgc acgacgcgag
      301 ctgcggggcg ggggggaact tcctgactag gggaggagta gaaggtggcg cgaaggggcc
      361 accaaagaac ggagccggtt ggcgcctacc ggtggatgtg gaatgtgtgc gaggccagag
      421 gccacttgtg tagcgccaag tgcccagcgg ggctgctaaa gcgcatgctc cagactgcct
      481 tgggaaaagc gcctccccta cccggtagaa ttgacctgca ggggccctcg acaaattgtt
      541 atccgctcac aattccacac tcgagatcct cggggtcttc cggggcgagt tctggctggc
      601 ttgcacgtgt atacagctgt gcggccgcaa gcttttacaa cagcaggcat tttctcttcc
      661 tcttcttcac gggaggcggg cagaggactg ctcggatcgc ttcgtcaaac actgtcttga
      721 ggcctcgctg tgtgagcgcc gagcactcca ggtatttt
//
