LOCUS       sequence-173277-        7109 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses sLucopt luciferase from the constitive Bacillus subtilis 
            promoter Pveg in Clostridium difficile.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7109)
  TITLE     Smits lab Constitutively secreted luciferase plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAP84
FEATURES             Location/Qualifiers
     source          1..7109
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(343..714)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            747..856
                     /note="incP origin of transfer"
     primer_bind     complement(2047..2064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2218..2806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2298..2317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatcctataa gttttaataa aactttaaat agaaaaaggc ttctctcatg agaagtcttt
       61 tttatttaaa ataaatataa aataaaatag aggctataaa tagcctctat tttatgtgag
      121 aaatccctaa ataaaaagat gccagtgtgc tggaattcgc ccttaggctg cgctagggac
      181 ctctttagct ccttggaagc tgtcagtagt aggggatcgg tcttgccttg ctcgtcggtg
      241 atgtacttca ccagctccgc gaagtcgctc ttcttgatgg agcgcatggg gacgtgcttg
      301 gcaatcacgc gcaccccccg gccgttttag cggctaaaaa agtcatggct ctgccctcgg
      361 gcggaccacg cccatcatga ccttgccaag ctcgtcctgc ttctcttcga tcttcgccag
      421 cagggcgagg atcgtggcat caccgaaccg cgccgtgcgc gggtcgtcgg tgagccagag
      481 tttcagcagg ccgcccaggc ggcccaggtc gccattgatg cgggccagct cgcggacgtg
      541 ctcatagtcc acgacgcccg tgattttgta gccctggccg acggccagca ggtaggccga
      601 caggctcatg ccggccgccg ccgccttttc ctcaatcgct cttcgttcgt ctggaaggca
      661 gtacaccttg ataggtgggc tgcccttcct ggttggcttg gtttcatcag ccatccgctt
      721 gccctcatct gttacgccgg cggtagccgg ccagcctcgc agagcaggat tcccgttgag
      781 caccgccagg tgcgaataag ggacagtgaa gaaggaacac ccgctcgcgg gtgggcctac
      841 ttcacctatc ctgcccggct gacgccgttg gatacaccaa ggaaagtcta cacgaaccct
      901 ttggcaaaat cctgtatatc gtgcgaaaaa ggatggatat accgaaaaaa tcgctataat
      961 gaccccgaag caggaagggc gaattctgca gatctgaccg gtctctgaaa atataaaaac
     1021 cacagattga tactaaaacc ttggttgtgt tgcttttcgg ggcttaaatc aaggaaaaat
     1081 ccttgtttta agcctttcaa aaagaaacac aaggtctttg tactaacctg tggttatgta
     1141 taaaattgta gattttaggg taacaaaaaa caccgtattt ctacgatgtt tttgcttaaa
     1201 tacttgtttt tagttacaga caaacctgaa gttaactatt tatcaattcc tgcaattcgt
     1261 ttacaaaacg gcaaatgtga aatccgtcac atactgcgtg atgaacttga attgccaaag
     1321 gaagtataat tttgttatct tctttataat atttccccat agtaaaaata ggaatcaaat
     1381 aatcatatcc tttctgcaaa ttcagattaa agccatcgaa ggttgaccac ggtatcatag
     1441 atacattaaa aatgttttcc ggagcatttg gctttccttc cattctatga ttgtttccat
     1501 accgttgcgt atcactttca taatctgcta aaaatgattt aaagtcagac ttacactcag
     1561 tccaaaggct ggaaaatgtt tcagtatcat tgtgaaatat tgtatagctt ggtatcatct
     1621 catcatatat ccccaattca ccatcttgat tgattgccgt cctaaactct gaatggcggt
     1681 ttacaatcat tgcaatataa taaagcattg caggatatag tttcattccc ttttccttta
     1741 tttgtgtgat atccacttta acggtcatgc tgtaggtaca aggtacactt gcaaagtagt
     1801 ggtcaaaata ctcttttctg ttccaactat ttttatcaat tttttcaaat accatctaag
     1861 ttccctctca aattcaagtt tatcgctcta atgaacaaag atattatacc acatttttgt
     1921 gaatttttca acttgcccac ttcgactgca ctcccgactt aataacttct tgaacacttg
     1981 ccgaaaaaga aaaactgccg ggtacgtacc cggggatcga tccccggccg agcgctcttc
     2041 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     2101 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     2161 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     2221 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     2281 aaacccgaca ggactataaa gataccaggc gtttccccct tgaagctccc tcgtgcgctc
     2341 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     2401 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     2461 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     2521 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     2581 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     2641 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     2701 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     2761 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     2821 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     2881 gaaatgcaag tttctaacta acatacatca tagttactaa actatggtaa ctataatttt
     2941 attaactata ttaagcactg attagtacta taactcaata taagcatatc ccctgtatcg
     3001 taactagaga accaaacgac ggaaaaagcg atatagataa gtttagatag tgcaccaatt
     3061 ttttactttt ctattttaga atctagcatt tccaatgctt actaatacta tttttgattt
     3121 ttaacttgtt ttaaaaaagc acctactaat taggattcta gctctatatt ctagttatac
     3181 attctctata ttctagctct atattctagt tatacattct ctatattcta gctctatatt
     3241 ctagttatac attctctata ttctagctct atattctagt tatacattct ctatattcta
     3301 gctctatatt ctagttatac attctctata ttctagctct atattctagt tatacattct
     3361 ctatattcta gctctatatt ctagttatac attctctata ttctagctct atattctagt
     3421 tatacattct ctaaaaaaca tatatatata tactacatat atatgtacaa ttattttacc
     3481 aaaagaaatt tttaaaacgg cttaatttca atactttgat gccatttttt ttttgcaatt
     3541 ctcgtacaat tattttacca aaagaaattt ttattaatta gtttttttgc ataaaaatag
     3601 gactagaaaa tcaattctaa tcctatcaaa aatctacttg tttaatctat atgtatagta
     3661 cataaatcct ctatcattta ttttagtttc ccatacatct aaaattttag tatctaataa
     3721 agattctatc atatttgaaa ttctttgctg tgttactcct aaatcttttg ctaattcctc
     3781 ttgattaatt cttaatatat ttccttttat tttatcatct ttgaccaata aatcttgctt
     3841 atatctcata tgagtatata atcttaattc ttcagttgaa attattcccc aaattactgc
     3901 catggtaaca aagtaactaa tagtatattg tttatctata ttacatctaa cttcttttac
     3961 attgtatgta tcttttattc ctagctttgt aacacctttt gtttttgtaa tataatcatt
     4021 atgttgaagt tcttttaatg tttctcttaa tgtcttttca ctcataacaa tgtttttaac
     4081 tttcttctta cgtttatagg ttatcagctc cattatatcg tctatgttta attctctatc
     4141 tttattgtta agtaacacat tatagataaa caattgatta ccagtcatta ttttaacccc
     4201 tttcctattc ttatatttca aatcctttga gtgcttatgt ggcatattga acaaagtatc
     4261 ttcatcacta tatataaaat ctgattctat tttattccaa caatcactat taaactcaca
     4321 acctgtacaa ccaaaattaa ccgttttaag cttctcatac atatacttga cttgatattc
     4381 tagctcacta tttgaaagac tattttgtga tatatagttc cattctctag caacggatat
     4441 tacttgactt aaacttttat tcctcaatct taaagtcaca actatttttt gcaaatctag
     4501 gtttttatgt ccatctttac taccattttt taaaatatct actatacaat ttggtagctt
     4561 actatctaat atatattttg tattagtatt acctactgtt tttacatctt tgcaatattt
     4621 tttagctaac ttttctatgt catatctata tatatttttg tcttggtgta ttatttttac
     4681 ttgtttagta gtatttttaa tattatatgt ataaggtact cttaatactt gagtagataa
     4741 atttgcttgt ttatctgcac ccaatttatt tattaatgta tttgtaactt ctgatactaa
     4801 cttaatgtta ttagttttat taattagcac ataaacatgg aatccatttc cactatcaac
     4861 taggatatga taatgtaatc ctatcttagt aaataaatta attatatctt tataattaaa
     4921 accttgtcct aattcttttt tgtcaaaatc aaatcctaat gcatatctat ttattaagtt
     4981 ttcattggtt cctgcttttc catcagttgt acataggtta aaaaatgtat tttcataata
     5041 aatatttttt gcgaaggtaa attctactat atcatctata tttttaaaaa aagttgtttt
     5101 actatattct ttaaattctt ctccgagttc taattgttta ctattacttt tattattttg
     5161 aaatactcta acatattcat tatcaataag attatttcct tgtcctatac catctctaaa
     5221 tactgccatt agaaattttt tcaacttata ttttgaatct aattgttcca tatatttatt
     5281 atctccttat ctagtttttt taagagataa cactacatta aatatatact tattgactaa
     5341 tcaactttta caactattga ataattttag cttagtatgt ataataatat tgttaagatg
     5401 ctaaaattga tagcaatatt attatataac tacagatact taatcagtta gaccgatatt
     5461 ggagttggta gctccataaa ctcaattggt agttgagttg tcattgtcta gtagtattat
     5521 ctcattttgt tgttatctta aaatattatt ttaaaaatac tctgcttggt cggcttaggg
     5581 tatttttttt gtttaattcc aatattggtt caaactcctg tgtaatactt ttgacacttc
     5641 catctgtatt gaattgacag ttgatatggt atctcattat tggcttgttc ttatgttttt
     5701 ctatgtattt attttttact ttcaaataat ttgtatttcc atttttagtt atgtttaaca
     5761 cactatcctt attactcaat aaaattattt tttgttttaa catatgattt atagaatcta
     5821 aaaattgata ttgacttata atagtacttt ctacctctct agctttttct agtgtttcaa
     5881 attcttcaaa ctctgctata ttgctatagt atagcttatc attaataata ttatctgaca
     5941 actttaattc cattttataa aacaattcca agttatgtat aaaattttta atgttacaac
     6001 atatagactt attatttgtt cttatatggt atctccattt actacttcta gctctcatgt
     6061 tatatgcaca actactccca acataaacat atattgcttg ttctaactca tatttttgta
     6121 aaatttcaac atcatatcca gtcaaatcgt actctctctt atatacaaat ggtttttcta
     6181 attttttgtt caataaatct cctcctattt cttacttaat ttagctaaaa atctatttat
     6241 atttcacctc ctttttgact ttaagcctac gaataccata tttatgatta taacatcttc
     6301 tatttttccc aaatccttac atctccccta atttttagac ttaagggcga attctgcatc
     6361 aagctagcat aaaaataaga agcctgcatt tgcaggcttc ttatttttat ggtaccctta
     6421 ttaacgttga tataatttaa attttatttg acaaaaatgg gctcgtgttg tacaataaat
     6481 gtagatctcc tgcagtaaag gagaaaattt tatgagacca agtaaaaaat tattaatagc
     6541 tataatatca atattcttaa taagttcagt tccagtaagt gcacatgcag atagtgtttt
     6601 tacacttgaa gattttgtgg gtgattggag acaaactgct ggatataatt tagatcaagt
     6661 tttagaacag ggtggagtga gttctctttt tcaaaatctt ggtgtctcag ttacaccaat
     6721 acaaagaata gttttaagtg gagaaaatgg attaaagatc gatatacatg taataattcc
     6781 ttatgaggga ctatcagggg accaaatggg acaaattgag aaaatattca aagttgtata
     6841 tccagtagac gatcatcact ttaaagtaat attacattat ggaactttag taattgatgg
     6901 tgtaacacca aatatgatag attattttgg tagaccatac gaagggattg cagtttttga
     6961 tggaaagaaa ataaccgtaa caggcacttt gtggaatgga aataaaataa tagatgaaag
     7021 gttaattaat cctgatggat ctttattgtt tagagtaact ataaacggtg ttacaggttg
     7081 gagactttgt gaacgtattc tagcatagg
//
