LOCUS       sequence-173284-        3854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  codes for QF2 (truncated QF) in a gateway middle entry backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3854)
  TITLE     Halpern Lab QF2 gateway middle entry
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pME-QF2
FEATURES             Location/Qualifiers
     source          1..3854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..30
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     22..39
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     23..39
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    55..154
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1243..1267)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    1269..1403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1293..1312)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1347..1366
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(1465..1564)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1581..1600)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1582..1600)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1605..1621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1605..1621)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1723..1742)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1734..2543
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1801..1820)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2690..3278
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3179..3198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3432..3449
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3608..3635)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3727..3813)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccccaaata
       61 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
      121 tttataatgc caactttgta caaaaaagca ggctcaacat gccacccaag cgcaaaacgc
      181 ttaacgctgc ggctgaggct aacgctcatg ccgacggaca cgccgacgga aacgccgacg
      241 gacacgtggc caatacggcc gcgtcctcga ataatgcgag gttcgctgat ctcactaaca
      301 tcgatactcc gggtctggga cccacaacta cgaccctgct cgtggaacca gcacgctcaa
      361 agcgtcaacg agtgtcccgc gcatgcgacc agtgccgtgc agcccgagag aaatgcgacg
      421 gaatacagcc tgcgtgtttc ccgtgcgttt cccagggaag gtcctgcact tatcaggctt
      481 cgccgaaaaa gaggggagtt caaaccggtt atattcgtac gctggagctc gccctcgcct
      541 ggatgtttga aaatgtcgcg cgttccgaag atgccttgca taacctcctc gtccgtgacg
      601 ccggacaagg atcagctctg ctcgttggta aagattcgcc ggctgccgag cgactccatg
      661 cccgttgggc tactagccgt gtcaataaga gcattacccg cctcctccgt cagttggagc
      721 tccctcctac cgccacggct acggcctcga taatgccgca cgtgatggag cagcctctca
      781 gtaccagcat taaccccgtc aacgaccgct tcaacggtat tcccaacccc actccgtata
      841 actccgatgc agctctcgat gctatcactc agaccaacga ttatggaagc gtaaatacac
      901 atggtatcct ctctacttac ccgccaccgg ctacgcacct taatgaagct tccgtcgctc
      961 tcgctcccgg tggcgccccc ccccgaccgc ctcctccgta tgttgacagc acgaccaatc
     1021 acccgccgta ccactcgaat ctggttccaa tggcgaactt tggttactcg accgttgatt
     1081 acgatgccat ggttgacgat ttggctagca ttgaatacac ggacgctgtg gatgtcgacc
     1141 cacagtttat gaccaatctg ggattcgttc ctggatgtaa cttctccgac attaatacat
     1201 acgaacagtg atgacgtcta tcgataccgt cgactaaagc caacccagct ttcttgtaca
     1261 aagtggggga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1321 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1381 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1441 agggggaggt gtgggaggtt ttttacccag ctttcttgta caaagttggc attataagaa
     1501 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
     1561 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct
     1621 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     1681 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc
     1741 atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg
     1801 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg
     1861 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     1921 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     1981 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa
     2041 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     2101 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     2161 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     2221 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac
     2281 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     2341 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     2401 gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga
     2461 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt
     2521 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca
     2581 ttacgctgac ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg
     2641 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2701 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2761 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2821 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2881 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2941 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3001 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3061 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3121 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3181 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3241 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3301 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3361 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3421 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3481 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3541 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3601 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3661 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     3721 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     3781 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     3841 taacgctagc atgg
//
