LOCUS       sequence-173288-        5001 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway donor vector for Spinophilin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Hiday AC, Edler MC, Salek AB, Morris CW, Thang M, Rentz TJ, Rose KL,
            Jones LM, Baucum AJ 2nd
  TITLE     Mechanisms and Consequences of Dopamine Depletion-Induced 
            Attenuation of the Spinophilin/Neurofilament Medium Interaction.
  JOURNAL   Neural Plast. 2017;2017:4153076. doi: 10.1155/2017/4153076. Epub 
            2017 May 28.
  PUBMED    28634551
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neural 
            Plast."; date: "2017"; volume: "2017:4153076. doi"; pages: " 
            10.1155/2017/4153076. Epub 2017 May 28"
COMMENT     Sequence Label: pDONR221 Spinophilin
FEATURES             Location/Qualifiers
     source          1..5001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(289..388)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(404..421)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(404..420)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(413..435)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      484..570
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      662..689
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(848..865)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1019..1607)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1099..1118)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1754..2563)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2477..2496
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2555..2574
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2676..2692
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2676..2692
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2697..2716
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2697..2715
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2733..2832
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gcagcgacag gcgcacgccg cgctcggacg cccgtggggc ctcggccagg cccgcgccgc
       61 cgcccgcctc gcccgagggc cccgccgtcg tgcccatctg caggaacata cttttgatgc
      121 ggtggacgtt ggagccatat ttcttgtggt gggccccctt gggtgcctcg tcgggcccgg
      181 gcgcgtcggg cggcttcagc gcctggatgc ccgcctcgta ggcgctgcgg tgcggggagg
      241 cgctccggag gggacccccg ggcccccgtg gctccgtctt catcattaag cctgcttttt
      301 tgtacaaagt tggcattata aaaaagcatt gctcatcaat ttgttgcaac gaacaggtca
      361 ctatcagtca aaataaaatc attatttggg gcccgagctt aagactggcc gtcgttttac
      421 aacgtcgtga ctgggaaaac atccatgcta gcgttaacgc gagagtaggg aactgccagg
      481 catcaaataa aacgaaaggc tcagtcggaa gactgggcct ttcgttttat ctgttgtttg
      541 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgtgaag
      601 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaactaag
      661 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttcc tggctagcgg
      721 tacgcgtatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      781 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      841 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      901 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      961 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1021 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1441 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1621 tcttttctac ggggtctgac gctcagtgga acgacgcgta actcacgtta agggattttg
     1681 gtcatgagct tgcgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat
     1741 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1801 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1861 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1921 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1981 catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt
     2041 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     2101 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     2161 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     2221 ctgaatcagg atattcttct aatacctgga atgctgtttt tccggggatc gcagtggtga
     2281 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     2341 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     2401 catgtttcag aaacaactct ggcgcatcgg gcttcccata caagcgatag attgtcgcac
     2461 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     2521 aatttaatcg cggcctcgac gtttcccgtt gaatatggct cataacaccc cttgtattac
     2581 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     2641 aacatcagag attttgagac acgggccaga gctgccagga aacagctatg accatgtaat
     2701 acgactcact ataggggata tcagctggat ggcaaataat gattttattt tgactgatag
     2761 tgacctgttc gttgcaacaa attgataagc aatgctttct tataatgcca actttgtaca
     2821 agaaagctgg gtattaagta gaattggaat tcctcagtgt ttgcaagttt ccttccagtt
     2881 ctgagatctt gtccaggagc ttgtccatct cctcctttct ggccagctcc gactcctcca
     2941 gaacccgacg ctgtgcagtc tcctttttca ggaactcgat ctccttctgc tggtagtcct
     3001 tgatgaggcg cttggccttg ctgtacttgc gctccagggc ctggtactgc gcctgagtct
     3061 cccgcaggtg ctcgtccaca gcctggcaca ggctctgggc ctcaccccag tagccttcca
     3121 gtttctccat gcgctccttg ttctcctcca cactctgctc caactgcgcc ttctccaccc
     3181 gccagcgccc cttctcctgc tccaggctct gcagctttct tttcagctgc tggatctctg
     3241 cctcagtgac cgcatgcttg atctggagct ccttgaactt gtgcaccagc ttctcgggct
     3301 ccatgtccac aggggacagt gcatcctcgt tctccgctag ctcaaacacc tcgatggcca
     3361 tctcaccacc cgggaacgtg gggctcagct cctcatcctc gtcagtggca tactctcccg
     3421 tctcctcgtc atcctcccca tactgggcgt atctctgctc catcatctcc cgctgccatc
     3481 gctcctgttc caaagtctgc tgaattagct gggccacttc gctctgctct cccggccgct
     3541 cccggccaat cataaaccgc actcggccct tggtgttccg gagcacagac gccgcgaagc
     3601 tctgggtcac tcccaccaga cttgttccat ccacctccac caggagatca ttcacctgga
     3661 tcctgccatc ccgatgggcc gcaccaccct ccgtcacggt cttgacgaag atacccagct
     3721 tctccaggcc catgtctgcc ccggcgccca tgccgatgat gctgatgccc aggccctcgg
     3781 agtccttctc cagctccaca gggaacagct ccaacctctc cacacgcttc tccagctcgt
     3841 actcagcaga ggctgccatg ggatccacat cctcgttgcg acgatcgtaa tcctcgttgg
     3901 agtaagtgct gaacacttgg atgggcgccg tgctgaaatg gatcttccgg ctcggggctg
     3961 ggtcctcctc ctccgacagc cccgggatct ccacgcaccc cgactcgggc tcgtaggggg
     4021 gctccccatc ctcctcgtcg tcttcgtcgt cctcctccag ggcactgccc gcagagtcct
     4081 ccccgagccc actgtaggcg ctcacgtcca ccaagtccgc ctccgagaag tcctccttct
     4141 tggattcatc tacctcctca ggggccacgt ccggggcccg gccattgccc acccccctct
     4201 ctggcggcgc taccgccgcc gcctccttct ccggggccgc ttgggccttt ggctcctcgg
     4261 gcgcggggct ggctgcagtt gccacggtgc tgccattctc cagggccgcg tggaccgtaa
     4321 cctcggcctg gattacctcc ccgggcgccg actcggcctc cgactccccg ctctcctcca
     4381 cctccaccgg cttaatcttg cgcacctccc ggggcttggg gggcggccgg gcaggggcca
     4441 ctcggtgctg cgggggctgc tgccctgcgg ggcatcgctc tttctcggcc ggggcatccc
     4501 ccgacggggc gggcggcggc ggcggcgggg gctggaacac ccgggaccgc ttgctgacca
     4561 gcttcgagtt gacctgggga acccctgcgg ccctggggag cccgggcccg cggtggaggc
     4621 cggtcctcga gtcggccttc tcgaagacgg cgctgagctg gctgaccgtg ggggacacgg
     4681 cgtcagcgtc cagcttgtcc agcgcctcgg tgctgccgtt gaagcgcacc acgacgtcca
     4741 gcttccggtc ctgcaggccg gcgcgctcct gcctcagcag ccgccgcgcc gcggcctcct
     4801 tgtcgccgcc tgcggccgct ggggcgctcc gttcgaacag cttccgcgtc tcctgcagcc
     4861 gggacggcgg gtgcggcggc ggcgcaggct gcgcggaggg cgcgggcttg gagtcgaagc
     4921 ggctcacgcg ctccgacacg ctggtgccca gcttcagcag ggcgctgtgg tccacgttct
     4981 cgttcaggct gctggcccgc g
//
