LOCUS       sequence-173290-       10799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression construct encoding CD81 and GFP from separate 
            promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10799)
  TITLE     Grove lab plasmids
REFERENCE   2  (bases 1 to 10799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDUAL CD81 (GFP)
FEATURES             Location/Qualifiers
     source          1..10799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             16..649
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    696..821
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1318..1551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1736..1780
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2065..2182
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2335..2742
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2753..2762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3565..3585)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3983..3994)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4030..4540
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4459..4477
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4555..4564
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4561..4563,4564..4566,4567..5280)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4561..4563,4564..4566,4567..5280)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4606..4627)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4867..4886)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5214..5235
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5376..5609
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5634..5652)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5635..5652)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5942..5961)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6175..6194)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6294..6316
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6354..6372)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6440..6544
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6545..6613,6614..7405)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6763..6782)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7576..8164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8065..8084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8318..8335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8405..8425)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8410..8739
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8590..8725
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9873..9938
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10068..10088
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10513..10647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10550..10569)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10604..10623
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
       61 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
      121 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
      181 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
      241 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      301 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      361 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      421 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      481 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      541 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      601 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      661 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      721 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      781 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      841 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      901 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      961 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1021 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1081 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1141 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1201 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1261 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1321 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1381 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1441 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1501 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1561 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1621 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1681 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1741 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1801 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1861 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1921 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1981 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2041 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2101 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2161 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat
     2221 cgataagctt gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc
     2281 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     2341 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     2401 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     2461 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     2521 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     2581 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     2641 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     2701 tcactcggcg cgccagtcct ccgacagact gagtcgcccg gggggatccg ccgccaccat
     2761 gggagtggag ggctgcacca agtgcatcaa gtacctgctc ttcgtcttca atttcgtctt
     2821 ctggctggct ggaggcgtga tcctgggtgt ggccctgtgg ctccgccatg acccgcagac
     2881 caccaacctc ctgtatctgg agctgggaga caagcccgcg cccaacacct tctatgtagg
     2941 catctacatc ctcatcgctg tgggcgctgt catgatgttc gttggcttcc tgggctgcta
     3001 cggggccatc caggaatccc agtgcctgct ggggacgttc ttcacctgcc tggtcatcct
     3061 gtttgcctgt gaggtggccg ccggcatctg gggctttgtc aacaaggacc agatcgccaa
     3121 ggatgtgaag cagttctatg accaggccct acagcaggcc gtggtggatg atgacgccaa
     3181 caacgccaag gctgtggtga agaccttcca cgagacgctt gactgctgtg gctccagcac
     3241 actgactgct ttgaccacct cagtgctcaa gaacaatttg tgtccctcgg gcagcaacat
     3301 catcagcaac ctcttcaagg aggactgcca ccagaagatc gatgacctct tctccgggaa
     3361 gctgtacctc atcggcattg ctgccatcgt ggtcgctgtg atcatgatct tcgagatgat
     3421 cctgagcatg gtgctgtgct gtggcatccg gaacagctcc gtgtactgag tcgacctgca
     3481 ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
     3541 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     3601 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     3661 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     3721 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     3781 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     3841 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     3901 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     3961 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggtcg
     4021 aattcccacg gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg
     4081 cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt
     4141 cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc
     4201 gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac
     4261 gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc
     4321 aatggcagcg cgccgaccgc gatgggctgt ggccaatagt ggctgctcag cagggcgcgc
     4381 cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc
     4441 cctgttcctg cccgcacggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt
     4501 cggctccctc gttgaccgaa tcaccgacct ctctccccag gacgcgtacc ggtcgccacc
     4561 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4621 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4681 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4741 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4801 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4861 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4921 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4981 aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa gcagaagaac
     5041 ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt gcagctcgcc
     5101 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     5161 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     5221 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     5281 actcgaggga attaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt
     5341 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     5401 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     5461 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5521 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5581 cttttagtca gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag
     5641 tgtcacctaa atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct
     5701 aacgacaata tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca
     5761 tctctctcgt aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt
     5821 aacgccgtcc cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag
     5881 atcctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc
     5941 gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc
     6001 gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc
     6061 tccttgcatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc
     6121 tgcttcctaa tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat
     6181 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
     6241 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     6301 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
     6361 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6421 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6481 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     6541 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6601 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6661 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6721 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     6781 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6841 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6901 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     6961 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     7021 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     7081 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     7141 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     7201 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     7261 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     7321 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     7381 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7441 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7501 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     7561 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     7621 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     7681 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     7741 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     7801 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     7861 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     7921 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     7981 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     8041 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     8101 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     8161 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     8221 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     8281 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     8341 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     8401 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8461 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8521 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8581 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8641 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8701 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc
     8761 ttgcgagata tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
     8821 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
     8881 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
     8941 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
     9001 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta
     9061 ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc
     9121 agctacgatc acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc
     9181 gaaggcttca tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt
     9241 gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg
     9301 gttgatgact atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg
     9361 ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg
     9421 ggcgttgttc tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc
     9481 tgcatccatg acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc
     9541 tgaaacctcg acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc
     9601 ccttgatggt aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg
     9661 gcgcggcatt gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc
     9721 cgaacgtacc gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat
     9781 ttatgagtgg gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     9841 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     9901 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     9961 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
    10021 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
    10081 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
    10141 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
    10201 tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt
    10261 ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct gctattaata
    10321 actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat
    10381 atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt
    10441 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
    10501 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
    10561 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    10621 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    10681 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    10741 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtac
//
