LOCUS       sequence-173292-       10724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression construct encoding Claudin-1 and GFP from 
            separate promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10724)
  TITLE     Grove lab plasmids
REFERENCE   2  (bases 1 to 10724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pDUAL CLDN (GFP)
FEATURES             Location/Qualifiers
     source          1..10724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             16..649
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    696..821
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1318..1551
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1736..1780
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2065..2182
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2335..2742
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      2753..2762
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3490..3510)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3908..3919)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        3955..4465
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4384..4402
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     regulatory      4480..4489
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4486..4488,4489..4491,4492..5205)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4486..4488,4489..4491,4492..5205)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4531..4552)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4792..4811)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5139..5160
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5301..5534
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(5559..5577)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5560..5577)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5867..5886)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6100..6119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6219..6241
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6279..6297)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6365..6469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6470..6538,6539..7330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6688..6707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7501..8089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7990..8009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8243..8260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8330..8350)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8335..8664
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8515..8650
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9798..9863
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9993..10013
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10438..10572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10475..10494)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10529..10548
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
       61 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
      121 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
      181 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
      241 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
      301 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
      361 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
      421 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
      481 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      541 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      601 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      661 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      721 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      781 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      841 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      901 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      961 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1021 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1081 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1141 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1201 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1261 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1321 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1381 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1441 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1501 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1561 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1621 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1681 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1741 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1801 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1861 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1921 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1981 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     2041 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2101 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2161 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat
     2221 cgataagctt gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc
     2281 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     2341 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     2401 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     2461 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     2521 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     2581 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     2641 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     2701 tcactcggcg cgccagtcct ccgacagact gagtcgcccg gggggatccg ccgccaccat
     2761 ggccaacgcg gggctgcagc tgttgggctt cattctcgcc ttcctgggat ggatcggcgc
     2821 catcgtcagc actgccctgc cccagtggag gatttactcc tatgccggcg acaacatcgt
     2881 gaccgcccag gccatgtacg aggggctgtg gatgtcctgc gtgtcgcaga gcaccgggca
     2941 gatccagtgc aaagtctttg actccttgct gaatctgagc agcacattgc aagcaacccg
     3001 tgccttgatg gtggttggca tcctcctggg agtgatagca atctttgtgg ccaccgttgg
     3061 catgaagtgt atgaagtgct tggaagacga tgaggtgcag aagatgagga tggctgtcat
     3121 tgggggcgcg atatttcttc ttgcaggtct ggctatttta gttgccacag catggtatgg
     3181 caatagaatc gttcaagaat tctatgaccc tatgacccca gtcaatgcca ggtacgaatt
     3241 tggtcaggct ctcttcactg gctgggctgc tgcttctctc tgccttctgg gaggtgccct
     3301 actttgctgt tcctgtcccc gaaaaacaac ctcttaccca acaccaaggc cctatccaaa
     3361 acctgcacct tccagcggga aagactacgt gtgagtcgac ctgcaggcat gcaagcttga
     3421 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3481 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3541 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3601 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3661 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3721 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3781 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     3841 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3901 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggtcgaattc ccacggggtt
     3961 ggggttgcgc cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg
     4021 tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg
     4081 cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc
     4141 gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc
     4201 cgcacgtctc actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg
     4261 accgcgatgg gctgtggcca atagtggctg ctcagcaggg cgcgccgaga gcagcggccg
     4321 ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc
     4381 acggtgttcc gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga
     4441 ccgaatcacc gacctctctc cccaggacgc gtaccggtcg ccaccatggt gagcaagggc
     4501 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4561 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4621 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4681 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4741 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4801 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4861 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4921 tacaacagcc acaaggtcta tatcaccgcc gacaagcaga agaacggcat caaggtgaac
     4981 ttcaagaccc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     5041 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     5101 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     5161 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaactcg agggaattaa
     5221 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     5281 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     5341 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     5401 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     5461 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     5521 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     5581 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     5641 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     5701 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     5761 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     5821 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     5881 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     5941 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     6001 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     6061 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     6121 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     6181 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     6241 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     6301 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     6361 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6421 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6481 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6541 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6601 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6661 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6721 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6781 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6841 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6901 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6961 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     7021 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     7081 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     7141 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     7201 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     7261 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7321 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7381 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7441 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7501 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7561 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7621 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7681 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7741 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7801 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7861 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7921 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7981 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     8041 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     8101 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     8161 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     8221 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     8281 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     8341 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     8401 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     8461 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     8521 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8581 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8641 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     8701 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     8761 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     8821 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     8881 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     8941 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     9001 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     9061 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     9121 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     9181 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     9241 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     9301 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     9361 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     9421 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     9481 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     9541 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     9601 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     9661 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     9721 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     9781 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     9841 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     9901 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     9961 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
    10021 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
    10081 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
    10141 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
    10201 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
    10261 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
    10321 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
    10381 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    10441 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10501 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
    10561 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
    10621 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
    10681 gaattatttt cattttaaag aaattgtatt tgttaaatat gtac
//
