LOCUS       sequence-173316-        7661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon 
            CH, Novina CD
  TITLE     The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved 
            SRA1-like Region.
  JOURNAL   Cell Rep. 2016 May 31;15(9):2025-37. doi: 
            10.1016/j.celrep.2016.04.018. Epub 2016 May 19.
  PUBMED    27210747
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.018"; journalName: "Cell Rep"; date: 
            "2016-05-31- 31"; volume: "15"; issue: "9"; pages: "2025-37"
COMMENT     Sequence Label: pCDNA3.1(-) + SLNCR1 _568-637
FEATURES             Location/Qualifiers
     source          1..7661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2638..2655)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2644..2868
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2914..3342
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3001..3020)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3211..3232
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3351..3371)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3356..3685
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3536..3671
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3598..3617
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3752..4546
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3806..3825)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4416..4435
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4720..4841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4757..4776)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4811..4830
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4890..4906)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4890..4906)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4903..4925)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4914..4930
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4938..4944,4945..4962,4963..4968))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4983..5004
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5121..5138)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5292..5880)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5372..5391)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6051..6842,6843..6911))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6674..6693
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6912..7016)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7091..7110)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7282..7661
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacgggccc
      301 tctagactcg agcggccgcc actgtgctgg atatctgcag aattccacca cactggacta
      361 gtggatccga gctcggtacc aagcttcccg ggatacttag gatccagggc gcgcaggcgg
      421 cgcgggtgcg cggtgcggcg ctggtatcca gaggacgcgg tcaccgcctc tggcatttgt
      481 cgttctgcgc ttctccgcaa ggaccctctg ttaggcaggc gcccaccgta agcctcccgg
      541 gccttgtgaa cctgcaaacc caagtctgag agacgatccg ccttcagcgc tttccagctt
      601 ggcagagagg ctttcccggc ggggatcttt ggttggcgct ggcgatgcgc ggggaagaaa
      661 ggcgaggagc ggcgtccagg ctgggtgatg tcccagcacg agtaggcggg atgcgctcgc
      721 ttggtcctcc gggcgcccgg tccctgcccg cgtcgcgcgc ccacccctgg ggacgagaag
      781 gcggccgcct gaggaccccc gcccgcgacc tccgcgagtc tggagcgcag aggacagggt
      841 ctggctgctc tttggccttg gatggaaagt ggggaattgg gtggggggct gcggacccct
      901 taacgtggat tacttggtgt gtatcagctg ggctcagaag acccacgacc tcttctccat
      961 ccgtggattg atacgtgtgg ccgccttttc cagcacggcc tcctgccttc ctggtgcact
     1021 ttttggagaa cgtggtggaa tcagaggttt ctggctgact cggtgggtgc tttgaaccag
     1081 gaaaggacaa gaaagaggat gggaaggact gatccacatt cccaccagga agtttagcag
     1141 aacccccgcg tgccacctgg accccttgga aggacctggc tcaggctgga ccacctcttg
     1201 agaggcagga gctctggatt tgatcaagaa ttctttgctg agcatggtgc ctcatgccta
     1261 taatcccaac actttgggag gccagtgtgg gaggatctct tgagcccagg agttcaagac
     1321 tagcctgggc aacacagaga gaccccatct ctaaaataat aataataata aaataaaaaa
     1381 ttagcagggc atggtggcat gtgcctgtag tcccagctac ccaggaggct gaggcaagag
     1441 gatggctgga gcctgggatg ttgaggctgc aatgaactgt gattacccca ctgcactcca
     1501 gcctgggcaa aagagcgaga gaccctgtct caaataataa taataataat aatcttattt
     1561 tggagaataa agagacctct ggatttgagg tgccatttgg gtagaaagaa aagacgttta
     1621 caccgagaaa tagtctgtgt tgccctgaag gagcagaggg atgcatcgct ggaggtgacc
     1681 tacagttgaa gaagactcat tatgacagac cttgtccttc ttccttgtgg aaagtgtttc
     1741 ctctgctgct actgctcatg agactcttcc ccctccctgt cccagggaac caaagggctt
     1801 tctaccacac cctttcttgc cccccgcctc ccatgtctgc tgtgcctttg tactcagcaa
     1861 ttcttgtttg ctccattatc ttccagccgg atacagagtg aatagttaac cacacttagg
     1921 tcaaatagga tctaaatttt tgttcctgct ccgtgtaaag aggccagtgt ttgtgtgttg
     1981 caagcagcct tggaatagta actcttctca tttgtttggg atctggccac caagttccag
     2041 aatgatacac ggatcagtgc agaagttcat caggctctcg gaccttaggg ctgttggaga
     2101 aggcttcagc agcagaactg atggtgaagg ctcgtgttct ccatcctcaa ctttctttgc
     2161 ttcgatcata cacaagaata catttggaag ggcaaaaaat gaacactgtc gttcattgca
     2221 gccgtgtttt gtgacacaga tgcacagtct gctgtgaaga ccttctctca agtggcattt
     2281 gggagtccat gccagatcat ggtgcttcat gagagactga cagctatcag gggttgtggc
     2341 acttagtgag gactctcctc ccccagtgtg tgctgatgac acatacacac ctgacaatag
     2401 cttgagtctt ctctgttcct tttactctgt agccaacata cacatgattt aaaacccttt
     2461 ctaaatatct atcatggttc atccttgtcc aaatgcagag tcagagctat ttgtacttca
     2521 ttattatttc caaggcgaat agttggcttt ctttttgcaa aaataattaa agtttttgta
     2581 tgttgcagtt gctctagata agtatcacgt gaagcttaag tttaaaccgc tgatcagcct
     2641 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     2701 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     2761 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     2821 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     2881 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     2941 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     3001 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     3061 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     3121 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     3181 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     3241 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     3301 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
     3361 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3421 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3481 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     3541 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     3601 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     3661 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
     3721 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     3781 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     3841 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     3901 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
     3961 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     4021 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     4081 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     4141 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     4201 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     4261 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     4321 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     4381 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     4441 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     4501 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     4561 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     4621 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     4681 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     4741 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     4801 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     4861 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     4921 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4981 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5041 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5101 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5161 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5221 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5281 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5341 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5401 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5461 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5521 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5581 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5641 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5701 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5761 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5821 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5881 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5941 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6001 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6181 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6901 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6961 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7021 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     7081 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     7141 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     7201 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     7261 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
     7321 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
     7381 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
     7441 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
     7501 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
     7561 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
     7621 cttggcagta catctacgta ttagtcatcg ctattaccat g
//
