LOCUS       sequence-173319-        7328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7328)
  AUTHORS   Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon 
            CH, Novina CD
  TITLE     The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved 
            SRA1-like Region.
  JOURNAL   Cell Rep. 2016 May 31;15(9):2025-37. doi: 
            10.1016/j.celrep.2016.04.018. Epub 2016 May 19.
  PUBMED    27210747
REFERENCE   2  (bases 1 to 7328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.018"; journalName: "Cell Rep"; date: 
            "2016-05-31- 31"; volume: "15"; issue: "9"; pages: "2025-37"
COMMENT     Sequence Label: pCDNA3.1(-) + SLNCR1 _884-1285
FEATURES             Location/Qualifiers
     source          1..7328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2305..2322)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2311..2535
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2581..3009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2668..2687)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2878..2899
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3018..3038)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3023..3352
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3203..3338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3265..3284
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3419..4213
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3473..3492)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4083..4102
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4387..4508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4424..4443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4478..4497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4557..4573)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4557..4573)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4570..4592)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4581..4597
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4605..4611,4612..4629,4630..4635))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4650..4671
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4788..4805)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4959..5547)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5039..5058)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5718..6509,6510..6578))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6341..6360
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6579..6683)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6758..6777)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6949..7328
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacgggccc
      301 tctagactcg agcggccgcc actgtgctgg atatctgcag aattccacca cactggacta
      361 gtggatccga gctcggtacc aagcttcccg ggatacttag gatccagggc gcgcaggcgg
      421 cgcgggtgcg cggtgcggcg ctggtatcca gaggacgcgg tcaccgcctc tggcatttgt
      481 cgttctgcgc ttctccgcaa ggaccctctg ttaggcaggc gcccaccgta agcctcccgg
      541 gccttgtgaa cctgcaaacc caagtctgag agacgatccg ccttcagcgc tttccagctt
      601 ggcagagagg ctttcccggc ggggatcttt ggttggcgct ggcgatgcgc ggggaagaaa
      661 ggcgaggagc ggcgtccagg ctgggtgatg tcccagcacg agtaggcggg atgcgctcgc
      721 ttggtcctcc gggcgcccgg tccctgcccg cgtcgcgcgc ccacccctgg ggacgagaag
      781 gcggccgcct gaggaccccc gcccgcgacc tccgcgagtc tggagcgcag aggacagggt
      841 ctggctgctc tttggccttg gatggaaagt ggggaattgg gtggggggct gcggacccct
      901 taacgtggat tacttggtgt gtatcagctg ggctcagaag acccacgacc tcttctccat
      961 ccgtggattg atttgttctg cttaacagct gggtcgccaa gctggaggta tttttccctc
     1021 tccaccctgg tcttctcctg taacgtgtgg ccgccttttc cagcacggcc tcctgccttc
     1081 ctggtgcact ttttggagaa cgtggtggaa tcagaggttt ctggctgact cggtgggtgc
     1141 tttgaaccag gaaaggacaa gaaagaggat gggaaggact gatccacatt cccaccagga
     1201 agtttagcag aacccccgcg tgccacctgg accccttgga aggacctggc tcaggctgga
     1261 ccacctcttg agaggcagga gctctggcac cgagaaatag tctgtgttgc cctgaaggag
     1321 cagagggatg catcgctgga ggtgacctac agttgaagaa gactcattat gacagacctt
     1381 gtccttcttc cttgtggaaa gtgtttcctc tgctgctact gctcatgaga ctcttccccc
     1441 tccctgtccc agggaaccaa agggctttct accacaccct ttcttgcccc ccgcctccca
     1501 tgtctgctgt gcctttgtac tcagcaattc ttgtttgctc cattatcttc cagccggata
     1561 cagagtgaat agttaaccac acttaggtca aataggatct aaatttttgt tcctgctccg
     1621 tgtaaagagg ccagtgtttg tgtgttgcaa gcagccttgg aatagtaact cttctcattt
     1681 gtttgggatc tggccaccaa gttccagaat gatacacgga tcagtgcaga agttcatcag
     1741 gctctcggac cttagggctg ttggagaagg cttcagcagc agaactgatg gtgaaggctc
     1801 gtgttctcca tcctcaactt tctttgcttc gatcatacac aagaatacat ttggaagggc
     1861 aaaaaatgaa cactgtcgtt cattgcagcc gtgttttgtg acacagatgc acagtctgct
     1921 gtgaagacct tctctcaagt ggcatttggg agtccatgcc agatcatggt gcttcatgag
     1981 agactgacag ctatcagggg ttgtggcact tagtgaggac tctcctcccc cagtgtgtgc
     2041 tgatgacaca tacacacctg acaatagctt gagtcttctc tgttcctttt actctgtagc
     2101 caacatacac atgatttaaa accctttcta aatatctatc atggttcatc cttgtccaaa
     2161 tgcagagtca gagctatttg tacttcatta ttatttccaa ggcgaatagt tggctttctt
     2221 tttgcaaaaa taattaaagt ttttgtatgt tgcagttgct ctagataagt atcacgtgaa
     2281 gcttaagttt aaaccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     2341 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     2401 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     2461 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     2521 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     2581 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     2641 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     2701 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     2761 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     2821 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     2881 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     2941 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     3001 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3061 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3121 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3181 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3241 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     3301 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     3361 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     3421 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     3481 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     3541 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     3601 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     3661 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     3721 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     3781 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     3841 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     3901 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     3961 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     4021 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     4081 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     4141 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     4201 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     4261 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     4321 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     4381 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4441 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4501 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     4561 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     4621 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     4681 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     4741 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     4801 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4861 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4921 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4981 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5041 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5101 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5161 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5221 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5281 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5341 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5401 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5461 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5521 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5581 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5641 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5701 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5761 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5821 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5881 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5941 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6001 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6061 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6121 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6181 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     6241 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     6301 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     6361 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     6421 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     6481 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     6541 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     6601 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     6661 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     6721 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     6781 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     6841 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     6901 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     6961 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     7021 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     7081 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     7141 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     7201 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     7261 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     7321 ttaccatg
//
