LOCUS       sequence-173322-        7493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Schmidt K, Joyce CE, Buquicchio F, Brown A, Ritz J, Distel RJ, Yoon 
            CH, Novina CD
  TITLE     The lncRNA SLNCR1 Mediates Melanoma Invasion through a Conserved 
            SRA1-like Region.
  JOURNAL   Cell Rep. 2016 May 31;15(9):2025-37. doi: 
            10.1016/j.celrep.2016.04.018. Epub 2016 May 19.
  PUBMED    27210747
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2016.04.018"; journalName: "Cell Rep"; date: 
            "2016-05-31- 31"; volume: "15"; issue: "9"; pages: "2025-37"
COMMENT     Sequence Label: pCDNA3.1(-) + SLNCR1 _462-699
FEATURES             Location/Qualifiers
     source          1..7493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2470..2487)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2476..2700
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2746..3174
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2833..2852)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3043..3064
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3183..3203)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3188..3517
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3368..3503
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     3430..3449
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             3584..4378
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3638..3657)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4248..4267
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4552..4673
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4589..4608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4643..4662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4722..4738)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4722..4738)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4735..4757)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4746..4762
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4770..4776,4777..4794,4795..4800))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4815..4836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4953..4970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5124..5712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5204..5223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5883..6674,6675..6743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6506..6525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6744..6848)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6923..6942)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7114..7493
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacgggccc
      301 tctagactcg agcggccgcc actgtgctgg atatctgcag aattccacca cactggacta
      361 gtggatccga gctcggtacc aagcttcccg ggatacttag gatccagggc gcgcaggcgg
      421 cgcgggtgcg cggtgcggcg ctggtatcca gaggacgcgg tcaccgcctc tggcatttgt
      481 cgttctgcgc ttctccgcaa ggaccctctg ttaggcaggc gcccaccgta agcctcccgg
      541 gccttgtgaa cctgcaaacc caagtctgag agacgatccg ccttcagcgc tttccagctt
      601 ggcagagagg ctttcccggc ggggatcttt ggttggcgct ggcgatgcgc ggggaagaaa
      661 ggcgaggagc ggcgtccagg ctgggtgatg tcccagcacg agtaggcggg atgcgctcgc
      721 ttggtcctcc gggcgcccgg tccctgcccg cgtcgcgcgc ccacccctgg ggacgagaag
      781 gcggccgcct gaggaccccc gcccgcgacc tccgcgagtc tggagcgcag aggacagggt
      841 ctggctgctc tttggccttg gatggagtgg aatcagaggt ttctggctga ctcggtgggt
      901 gctttgaacc aggaaaggac aagaaagagg atgggaagga ctgatccaca ttcccaccag
      961 gaagtttagc agaacccccg cgtgccacct ggaccccttg gaaggacctg gctcaggctg
     1021 gaccacctct tgagaggcag gagctctgga tttgatcaag aattctttgc tgagcatggt
     1081 gcctcatgcc tataatccca acactttggg aggccagtgt gggaggatct cttgagccca
     1141 ggagttcaag actagcctgg gcaacacaga gagaccccat ctctaaaata ataataataa
     1201 taaaataaaa aattagcagg gcatggtggc atgtgcctgt agtcccagct acccaggagg
     1261 ctgaggcaag aggatggctg gagcctggga tgttgaggct gcaatgaact gtgattaccc
     1321 cactgcactc cagcctgggc aaaagagcga gagaccctgt ctcaaataat aataataata
     1381 ataatcttat tttggagaat aaagagacct ctggatttga ggtgccattt gggtagaaag
     1441 aaaagacgtt tacaccgaga aatagtctgt gttgccctga aggagcagag ggatgcatcg
     1501 ctggaggtga cctacagttg aagaagactc attatgacag accttgtcct tcttccttgt
     1561 ggaaagtgtt tcctctgctg ctactgctca tgagactctt ccccctccct gtcccaggga
     1621 accaaagggc tttctaccac accctttctt gccccccgcc tcccatgtct gctgtgcctt
     1681 tgtactcagc aattcttgtt tgctccatta tcttccagcc ggatacagag tgaatagtta
     1741 accacactta ggtcaaatag gatctaaatt tttgttcctg ctccgtgtaa agaggccagt
     1801 gtttgtgtgt tgcaagcagc cttggaatag taactcttct catttgtttg ggatctggcc
     1861 accaagttcc agaatgatac acggatcagt gcagaagttc atcaggctct cggaccttag
     1921 ggctgttgga gaaggcttca gcagcagaac tgatggtgaa ggctcgtgtt ctccatcctc
     1981 aactttcttt gcttcgatca tacacaagaa tacatttgga agggcaaaaa atgaacactg
     2041 tcgttcattg cagccgtgtt ttgtgacaca gatgcacagt ctgctgtgaa gaccttctct
     2101 caagtggcat ttgggagtcc atgccagatc atggtgcttc atgagagact gacagctatc
     2161 aggggttgtg gcacttagtg aggactctcc tcccccagtg tgtgctgatg acacatacac
     2221 acctgacaat agcttgagtc ttctctgttc cttttactct gtagccaaca tacacatgat
     2281 ttaaaaccct ttctaaatat ctatcatggt tcatccttgt ccaaatgcag agtcagagct
     2341 atttgtactt cattattatt tccaaggcga atagttggct ttctttttgc aaaaataatt
     2401 aaagtttttg tatgttgcag ttgctctaga taagtatcac gtgaagctta agtttaaacc
     2461 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     2521 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     2581 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     2641 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     2701 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     2761 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2821 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2881 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2941 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3001 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3061 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3121 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     3181 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     3241 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     3301 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3361 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3421 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     3481 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     3541 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     3601 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3661 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3721 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     3781 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3841 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3901 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3961 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     4021 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     4081 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     4141 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     4201 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     4261 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     4321 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     4381 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     4441 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     4501 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     4561 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     4621 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     4681 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4741 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4801 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4861 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4921 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5281 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5521 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6841 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     6901 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     6961 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     7021 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     7081 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     7141 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     7201 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     7261 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7321 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7381 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7441 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atg
//
