LOCUS       sequence-173323-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS 
            BELOW..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Morozova KS, Piatkevich KD, Gould TJ, Zhang J, Bewersdorf J, 
            Verkhusha VV
  TITLE     Far-red fluorescent protein excitable with red lasers for flow 
            cytometry and superresolution STED nanoscopy.
  JOURNAL   Biophys J. 2010 Jul 21;99(2):L13-5.
  PUBMED    20643047
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys 
            J."; date: "2010-07-21"; volume: "99(2)"; pages: "L13-5"
COMMENT     Sequence Label: pTagRFP657-C1
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    127..248
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(138..157)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     192..211
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    372..437
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
       61 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
      121 acgcgttaag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      181 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      241 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      301 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatcagttat
      361 ctagatccgg tggatcccgg gcccgcggta ccgtcgactg cagaattcga agcttgagct
      421 cgagatctga gtccggaatt aagcttgtgc cccagtttgc tagggaggtc gcagtatctg
      481 gccacagcca cctcgtgctg ctcgacgtag gtctctttgt cggcctcctt gattctttcc
      541 agtctgtagt ccacatagta gacgccgggc atcttgaggt tcttagcggg tttcttggat
      601 ctgtatgtgg tcttgaagtt gcagatcagg tggcccccgc ccacgagctt cagggccagc
      661 gcggttctgc cttccaggcc gccgtcagcg gggtacagca tctcggtgtg ggcctcccag
      721 ccgagtgttt tcttc
//
