LOCUS       sequence-173327-        7464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of human CSF-1(256).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Heard JM, Roussel MF, Rettenmier CW, Sherr CJ
  TITLE     Synthesis, post-translational processing, and autocrine transforming
            activity of a carboxylterminal truncated form of colony stimulating 
            factor-1.
  JOURNAL   Oncogene Res. 1987 Sep-Oct;1(4):423-40.
  PUBMED    2966924
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene 
            Res. 1987 Sep-Oct"; volume: "1(4)"; pages: "423-40"
COMMENT     Sequence Label: MSCV(pBabemcs)-human CSF-1(256)-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..7464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    934..1483
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1078..1095)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1305..1324
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(1486..1488,1489..1491,1492..2205)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1486..1488,1489..1491,1492..2202)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1531..1552)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1792..1811)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2139..2160
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             2367..2881
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    3043..3059
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3067..3073,3074..3091,3092..3097))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3112..3133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3250..3267)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3421..4009)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3501..3520)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4180..4971,4972..5040))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4803..4822
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5041..5145)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5213..5231
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5269..5291)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5391..5410
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5604..5626
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5754..5773
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             5973..6489
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    6553..6894
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             6961..7377
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     7307..7329
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     7345..7361
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 agccgctctc cgcatcccag gacagcggtg cggccctcgg ccggggcgcc cactccgcag
       61 cagccagcga gcgagcgagc gagcgagggc ggccgacgcg cccggccggg acccagctgc
      121 ccgtatgacc gcgccgggcg ccgccgggcg ctgccctccc acgacatggc tgggctccct
      181 gctgttgttg gtctgtctcc tggcgagcag gagtatcacc gaggaggtgt cggagtactg
      241 tagccacatg attgggagtg gacacctgca gtctctgcag cggctgattg acagtcagat
      301 ggagacctcg tgccaaatta catttgagtt tgtagaccag gaacagttga aagatccagt
      361 gtgctacctt aagaaggcat ttctcctggt acaatacata atggaggaca ccatgcgctt
      421 cagagataac acccccaatg ccatcgccat tgtgcagctg caggaactct ctttgaggct
      481 gaagagctgc ttcaccaagg attatgaaga gcatgacaag gcctgcgtcc gaactttcta
      541 tgagacacct ctccagttgc tggagaaggt caagaatgtc tttaatgaaa caaagaatct
      601 ccttgacaag gactggaata ttttcagcaa gaactgcaac aacagctttg ctgaatgctc
      661 cagccaaggc catgagaggc agtccgaggg atcctccagc ccgcagctcc aggagtctgt
      721 cttccacctg ctggtgccca gtgtcatcct ggtcttgctg gccgtcggag gcctcttgtt
      781 ctacaggtgg aggcggcgga gccatcaaga gcctcagaga gcggattctc ccttggagca
      841 accagagggc agccccctga ctcaggatga cagacaggtg gaactgccag tgtagaggga
      901 acctcgagcg ggatcaattc cgcccccccc ctaacgttac tggccgaagc cgcttggaat
      961 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     1021 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     1081 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     1141 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     1201 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     1261 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     1321 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     1381 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     1441 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgata ataccatggt gagcaagggc
     1501 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1561 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1621 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     1681 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     1741 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1801 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1861 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1921 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1981 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     2041 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     2101 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     2161 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct
     2221 agagtcgacc tgcaggcatg caagcttcag gtagccggct aacgttaaca accggtacct
     2281 ctagaactat agctagcatg cgcaaattta aagcgctgat atcgataaaa taaaagattt
     2341 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     2401 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     2461 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     2521 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     2581 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     2641 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     2701 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     2761 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     2821 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     2881 tgggtaacag tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag
     2941 taatcctgac tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt
     3001 tcattatgga cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat
     3061 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     3121 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     3181 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     3241 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3301 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3361 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3421 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3481 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3541 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3601 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3661 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3721 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3781 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3841 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3901 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3961 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4021 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4081 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4141 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4201 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4261 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4321 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4381 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4441 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4501 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4561 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4621 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4681 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4741 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4801 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4861 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4921 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4981 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5041 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5101 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5161 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     5221 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     5281 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     5341 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     5401 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5461 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     5521 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     5581 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat
     5641 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     5701 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     5761 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     5821 ccggcgtaga ggcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta
     5881 gttttgactc aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa
     5941 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc
     6001 aagctagctt aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga
     6061 agttcagatc aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt
     6121 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg
     6181 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     6241 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     6301 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     6361 agactgcgtc gcccgggtac ccgtattccc aataaagcct cttgctgttt gcatccgaat
     6421 cgtggactcg ctgatccttg ggagggtctc ctcagattga ttgactgccc acctcggggg
     6481 tctttcattt ggaggttcca ccgagatttg gagacccctg cccagggacc accgaccccc
     6541 ccgccgggag gtaagctggc cagcggtcgt ttcgtgtctg tctctgtctt tgtgcgtgtt
     6601 tgtgccggca tctaatgttt gcgcctgcgt ctgtactagt tagctaacta gctctgtatc
     6661 tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc tgggagacgt
     6721 cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc gatgtggaat
     6781 ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct
     6841 ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc
     6901 tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag
     6961 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
     7021 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
     7081 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
     7141 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     7201 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     7261 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     7321 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccgga
     7381 attgggatcc cagtgtggtg gtacgtagga attcagatct cgagggggga aagtgaaagt
     7441 ttgcctgggt cctctcggcg ccag
//
